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Differential target multiplexed spinal cord stimulation programming modulates proteins involved in ion regulation in an animal model of neuropathic pain
The effect of spinal cord stimulation (SCS) using differential target multiplexed programming (DTMP) on proteins involved in the regulation of ion transport in spinal cord (SC) tissue of an animal model of neuropathic pain was evaluated in comparison to low rate (LR) SCS. Rats subjected to the spare...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
SAGE Publications
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8785327/ https://www.ncbi.nlm.nih.gov/pubmed/35048719 http://dx.doi.org/10.1177/17448069211060181 |
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author | Tilley, Dana M Cedeño, David L Vetri, Francesco Platt, David C Vallejo, Ricardo |
author_facet | Tilley, Dana M Cedeño, David L Vetri, Francesco Platt, David C Vallejo, Ricardo |
author_sort | Tilley, Dana M |
collection | PubMed |
description | The effect of spinal cord stimulation (SCS) using differential target multiplexed programming (DTMP) on proteins involved in the regulation of ion transport in spinal cord (SC) tissue of an animal model of neuropathic pain was evaluated in comparison to low rate (LR) SCS. Rats subjected to the spared nerve injury model (SNI) and implanted with a SCS lead were assigned to DTMP or LR and stimulated for 48 h. A No-SCS group received no stimulation, and a Sham group received no SNI or stimulation. Proteins in the dorsal ipsilateral quadrant of the stimulated SC were identified and quantified using mass spectrometry. Proteins significantly modulated by DTMP or LR relative to No-SCS were identified. Bioinformatic tools were used to identify proteins related to ion transport regulation. DTMP modulated a larger number of proteins than LR. More than 40 proteins significantly involved in the regulation of chloride (Cl(−)), potassium (K(+)), sodium (Na(+)), or calcium (Ca(2+)) ions were identified. SNI affected proteins that promote the increase of intracellular Ca(2+), Na(+), and K(+) and decrease of intracellular Cl(−). DTMP modulated proteins involved in glial response to neural injury that affect Ca(2+) signaling. DTMP decreased levels of proteins related to Ca(2+) transport that may result in the reduction of intracellular Ca(2+). Presynaptic proteins involved in GABA vesicle formation and release were upregulated by DTMP. DTMP also upregulated postsynaptic proteins involved with elevated intracellular Cl(−), while modulating proteins, expressed by astrocytes, that regulate postsynaptic Cl(−) inhibition. DTMP downregulated K(+) regulatory proteins affected by SNI that affect neuronal depolarization, and upregulated proteins that are associated with a decrease of intracellular neuronal K(+) and astrocyte uptake of extracellular K(+). DTMP treatment modulated the expression of proteins with the potential to facilitate a reversal of dysregulation of ion transport and signaling associated with a model of neuropathic pain. |
format | Online Article Text |
id | pubmed-8785327 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-87853272022-01-25 Differential target multiplexed spinal cord stimulation programming modulates proteins involved in ion regulation in an animal model of neuropathic pain Tilley, Dana M Cedeño, David L Vetri, Francesco Platt, David C Vallejo, Ricardo Mol Pain Research Article The effect of spinal cord stimulation (SCS) using differential target multiplexed programming (DTMP) on proteins involved in the regulation of ion transport in spinal cord (SC) tissue of an animal model of neuropathic pain was evaluated in comparison to low rate (LR) SCS. Rats subjected to the spared nerve injury model (SNI) and implanted with a SCS lead were assigned to DTMP or LR and stimulated for 48 h. A No-SCS group received no stimulation, and a Sham group received no SNI or stimulation. Proteins in the dorsal ipsilateral quadrant of the stimulated SC were identified and quantified using mass spectrometry. Proteins significantly modulated by DTMP or LR relative to No-SCS were identified. Bioinformatic tools were used to identify proteins related to ion transport regulation. DTMP modulated a larger number of proteins than LR. More than 40 proteins significantly involved in the regulation of chloride (Cl(−)), potassium (K(+)), sodium (Na(+)), or calcium (Ca(2+)) ions were identified. SNI affected proteins that promote the increase of intracellular Ca(2+), Na(+), and K(+) and decrease of intracellular Cl(−). DTMP modulated proteins involved in glial response to neural injury that affect Ca(2+) signaling. DTMP decreased levels of proteins related to Ca(2+) transport that may result in the reduction of intracellular Ca(2+). Presynaptic proteins involved in GABA vesicle formation and release were upregulated by DTMP. DTMP also upregulated postsynaptic proteins involved with elevated intracellular Cl(−), while modulating proteins, expressed by astrocytes, that regulate postsynaptic Cl(−) inhibition. DTMP downregulated K(+) regulatory proteins affected by SNI that affect neuronal depolarization, and upregulated proteins that are associated with a decrease of intracellular neuronal K(+) and astrocyte uptake of extracellular K(+). DTMP treatment modulated the expression of proteins with the potential to facilitate a reversal of dysregulation of ion transport and signaling associated with a model of neuropathic pain. SAGE Publications 2022-01-20 /pmc/articles/PMC8785327/ /pubmed/35048719 http://dx.doi.org/10.1177/17448069211060181 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Research Article Tilley, Dana M Cedeño, David L Vetri, Francesco Platt, David C Vallejo, Ricardo Differential target multiplexed spinal cord stimulation programming modulates proteins involved in ion regulation in an animal model of neuropathic pain |
title | Differential target multiplexed spinal cord stimulation programming modulates proteins involved in ion regulation in an animal model of neuropathic pain |
title_full | Differential target multiplexed spinal cord stimulation programming modulates proteins involved in ion regulation in an animal model of neuropathic pain |
title_fullStr | Differential target multiplexed spinal cord stimulation programming modulates proteins involved in ion regulation in an animal model of neuropathic pain |
title_full_unstemmed | Differential target multiplexed spinal cord stimulation programming modulates proteins involved in ion regulation in an animal model of neuropathic pain |
title_short | Differential target multiplexed spinal cord stimulation programming modulates proteins involved in ion regulation in an animal model of neuropathic pain |
title_sort | differential target multiplexed spinal cord stimulation programming modulates proteins involved in ion regulation in an animal model of neuropathic pain |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8785327/ https://www.ncbi.nlm.nih.gov/pubmed/35048719 http://dx.doi.org/10.1177/17448069211060181 |
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