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An Iron Transporter Is Involved in Iron Homeostasis, Energy Metabolism, Oxidative Stress, and Metacyclogenesis in Trypanosoma cruzi
The parasite Trypanosoma cruzi causes Chagas’ disease; both heme and ionic Fe are required for its optimal growth, differentiation, and invasion. Fe is an essential cofactor in many metabolic pathways. Fe is also harmful due to catalyzing the formation of reactive O(2) species; for this reason, all...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8785980/ https://www.ncbi.nlm.nih.gov/pubmed/35083166 http://dx.doi.org/10.3389/fcimb.2021.789401 |
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author | Dick, Claudia F. Rocco-Machado, Nathália Dos-Santos, André L. A. Carvalho-Kelly, Luiz F. Alcantara, Carolina L. Cunha-E-Silva, Narcisa L. Meyer-Fernandes, José R. Vieyra, Adalberto |
author_facet | Dick, Claudia F. Rocco-Machado, Nathália Dos-Santos, André L. A. Carvalho-Kelly, Luiz F. Alcantara, Carolina L. Cunha-E-Silva, Narcisa L. Meyer-Fernandes, José R. Vieyra, Adalberto |
author_sort | Dick, Claudia F. |
collection | PubMed |
description | The parasite Trypanosoma cruzi causes Chagas’ disease; both heme and ionic Fe are required for its optimal growth, differentiation, and invasion. Fe is an essential cofactor in many metabolic pathways. Fe is also harmful due to catalyzing the formation of reactive O(2) species; for this reason, all living systems develop mechanisms to control the uptake, metabolism, and storage of Fe. However, there is limited information available on Fe uptake by T. cruzi. Here, we identified a putative 39-kDa Fe transporter in T. cruzi genome, TcIT, homologous to the Fe transporter in Leishmania amazonensis and Arabidopsis thaliana. Epimastigotes grown in Fe-depleted medium have increased TcIT transcription compared with controls grown in regular medium. Intracellular Fe concentration in cells maintained in Fe-depleted medium is lower than in controls, and there is a lower O(2) consumption. Epimastigotes overexpressing TcIT, which was encountered in the parasite plasma membrane, have high intracellular Fe content, high O(2) consumption—especially in phosphorylating conditions, high intracellular ATP, very high H(2)O(2) production, and stimulated transition to trypomastigotes. The investigation of the mechanisms of Fe transport at the cellular and molecular levels will assist in elucidating Fe metabolism in T. cruzi and the involvement of its transport in the differentiation from epimastigotes to trypomastigotes, virulence, and maintenance/progression of the infection. |
format | Online Article Text |
id | pubmed-8785980 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-87859802022-01-25 An Iron Transporter Is Involved in Iron Homeostasis, Energy Metabolism, Oxidative Stress, and Metacyclogenesis in Trypanosoma cruzi Dick, Claudia F. Rocco-Machado, Nathália Dos-Santos, André L. A. Carvalho-Kelly, Luiz F. Alcantara, Carolina L. Cunha-E-Silva, Narcisa L. Meyer-Fernandes, José R. Vieyra, Adalberto Front Cell Infect Microbiol Cellular and Infection Microbiology The parasite Trypanosoma cruzi causes Chagas’ disease; both heme and ionic Fe are required for its optimal growth, differentiation, and invasion. Fe is an essential cofactor in many metabolic pathways. Fe is also harmful due to catalyzing the formation of reactive O(2) species; for this reason, all living systems develop mechanisms to control the uptake, metabolism, and storage of Fe. However, there is limited information available on Fe uptake by T. cruzi. Here, we identified a putative 39-kDa Fe transporter in T. cruzi genome, TcIT, homologous to the Fe transporter in Leishmania amazonensis and Arabidopsis thaliana. Epimastigotes grown in Fe-depleted medium have increased TcIT transcription compared with controls grown in regular medium. Intracellular Fe concentration in cells maintained in Fe-depleted medium is lower than in controls, and there is a lower O(2) consumption. Epimastigotes overexpressing TcIT, which was encountered in the parasite plasma membrane, have high intracellular Fe content, high O(2) consumption—especially in phosphorylating conditions, high intracellular ATP, very high H(2)O(2) production, and stimulated transition to trypomastigotes. The investigation of the mechanisms of Fe transport at the cellular and molecular levels will assist in elucidating Fe metabolism in T. cruzi and the involvement of its transport in the differentiation from epimastigotes to trypomastigotes, virulence, and maintenance/progression of the infection. Frontiers Media S.A. 2022-01-10 /pmc/articles/PMC8785980/ /pubmed/35083166 http://dx.doi.org/10.3389/fcimb.2021.789401 Text en Copyright © 2022 Dick, Rocco-Machado, Dos-Santos, Carvalho-Kelly, Alcantara, Cunha-E-Silva, Meyer-Fernandes and Vieyra https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cellular and Infection Microbiology Dick, Claudia F. Rocco-Machado, Nathália Dos-Santos, André L. A. Carvalho-Kelly, Luiz F. Alcantara, Carolina L. Cunha-E-Silva, Narcisa L. Meyer-Fernandes, José R. Vieyra, Adalberto An Iron Transporter Is Involved in Iron Homeostasis, Energy Metabolism, Oxidative Stress, and Metacyclogenesis in Trypanosoma cruzi |
title | An Iron Transporter Is Involved in Iron Homeostasis, Energy Metabolism, Oxidative Stress, and Metacyclogenesis in Trypanosoma cruzi
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title_full | An Iron Transporter Is Involved in Iron Homeostasis, Energy Metabolism, Oxidative Stress, and Metacyclogenesis in Trypanosoma cruzi
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title_fullStr | An Iron Transporter Is Involved in Iron Homeostasis, Energy Metabolism, Oxidative Stress, and Metacyclogenesis in Trypanosoma cruzi
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title_full_unstemmed | An Iron Transporter Is Involved in Iron Homeostasis, Energy Metabolism, Oxidative Stress, and Metacyclogenesis in Trypanosoma cruzi
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title_short | An Iron Transporter Is Involved in Iron Homeostasis, Energy Metabolism, Oxidative Stress, and Metacyclogenesis in Trypanosoma cruzi
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title_sort | iron transporter is involved in iron homeostasis, energy metabolism, oxidative stress, and metacyclogenesis in trypanosoma cruzi |
topic | Cellular and Infection Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8785980/ https://www.ncbi.nlm.nih.gov/pubmed/35083166 http://dx.doi.org/10.3389/fcimb.2021.789401 |
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