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The adult microbiome of healthy and otitis patients: Definition of the core healthy and diseased ear microbiomes

Otitis media (OM) and externa (OE) are painful, recurrent ear conditions. As most otitis publications focus on the bacterial content of childhood ears, there remains a dearth of information regarding the adult ear microbiome including both bacteria and fungi. This study compares the outer ear microb...

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Autores principales: Burton, Maria, Krumbeck, Janina A., Wu, Guangxi, Tang, Shuiquan, Prem, Aishani, Gupta, Aditya K., Dawson, Thomas L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8786117/
https://www.ncbi.nlm.nih.gov/pubmed/35073343
http://dx.doi.org/10.1371/journal.pone.0262806
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author Burton, Maria
Krumbeck, Janina A.
Wu, Guangxi
Tang, Shuiquan
Prem, Aishani
Gupta, Aditya K.
Dawson, Thomas L.
author_facet Burton, Maria
Krumbeck, Janina A.
Wu, Guangxi
Tang, Shuiquan
Prem, Aishani
Gupta, Aditya K.
Dawson, Thomas L.
author_sort Burton, Maria
collection PubMed
description Otitis media (OM) and externa (OE) are painful, recurrent ear conditions. As most otitis publications focus on the bacterial content of childhood ears, there remains a dearth of information regarding the adult ear microbiome including both bacteria and fungi. This study compares the outer ear microbiome of healthy adults to adults affected by OE and OM using both intergenic-transcribed-spacer (ITS) and 16S-rDNA sequencing. The adult ear core microbiome consists of the prokaryote Cutibacterium acnes and the eukaryotic Malassezia arunalokei, M. globosa, and M. restricta. The healthy ear mycobiome is dominated by Malassezia and can be divided into two groups, one dominated by M. arunalokei, the other by M. restricta. Microbiome diversity and biomass varied significantly between healthy and diseased ears, and analyses reveal the presence of a potential mutualistic, protective effect of Malassezia species and C. acnes. The healthy ear core microbiome includes the bacteria Staphylococcus capitis and S. capitis/caprae, while the diseased ear core is composed of known bacterial and fungal pathogens including Aspergillus sp., Candida sp., Pseudomonas aeruginosa, S. aureus, and Corynebacterium jeikeium. The data presented highlight the need for early detection of the cause of otitis to direct more appropriate, efficient treatments. This will improve patient outcomes and promote improved antimicrobial stewardship.
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spelling pubmed-87861172022-01-25 The adult microbiome of healthy and otitis patients: Definition of the core healthy and diseased ear microbiomes Burton, Maria Krumbeck, Janina A. Wu, Guangxi Tang, Shuiquan Prem, Aishani Gupta, Aditya K. Dawson, Thomas L. PLoS One Research Article Otitis media (OM) and externa (OE) are painful, recurrent ear conditions. As most otitis publications focus on the bacterial content of childhood ears, there remains a dearth of information regarding the adult ear microbiome including both bacteria and fungi. This study compares the outer ear microbiome of healthy adults to adults affected by OE and OM using both intergenic-transcribed-spacer (ITS) and 16S-rDNA sequencing. The adult ear core microbiome consists of the prokaryote Cutibacterium acnes and the eukaryotic Malassezia arunalokei, M. globosa, and M. restricta. The healthy ear mycobiome is dominated by Malassezia and can be divided into two groups, one dominated by M. arunalokei, the other by M. restricta. Microbiome diversity and biomass varied significantly between healthy and diseased ears, and analyses reveal the presence of a potential mutualistic, protective effect of Malassezia species and C. acnes. The healthy ear core microbiome includes the bacteria Staphylococcus capitis and S. capitis/caprae, while the diseased ear core is composed of known bacterial and fungal pathogens including Aspergillus sp., Candida sp., Pseudomonas aeruginosa, S. aureus, and Corynebacterium jeikeium. The data presented highlight the need for early detection of the cause of otitis to direct more appropriate, efficient treatments. This will improve patient outcomes and promote improved antimicrobial stewardship. Public Library of Science 2022-01-24 /pmc/articles/PMC8786117/ /pubmed/35073343 http://dx.doi.org/10.1371/journal.pone.0262806 Text en © 2022 Burton et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Burton, Maria
Krumbeck, Janina A.
Wu, Guangxi
Tang, Shuiquan
Prem, Aishani
Gupta, Aditya K.
Dawson, Thomas L.
The adult microbiome of healthy and otitis patients: Definition of the core healthy and diseased ear microbiomes
title The adult microbiome of healthy and otitis patients: Definition of the core healthy and diseased ear microbiomes
title_full The adult microbiome of healthy and otitis patients: Definition of the core healthy and diseased ear microbiomes
title_fullStr The adult microbiome of healthy and otitis patients: Definition of the core healthy and diseased ear microbiomes
title_full_unstemmed The adult microbiome of healthy and otitis patients: Definition of the core healthy and diseased ear microbiomes
title_short The adult microbiome of healthy and otitis patients: Definition of the core healthy and diseased ear microbiomes
title_sort adult microbiome of healthy and otitis patients: definition of the core healthy and diseased ear microbiomes
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8786117/
https://www.ncbi.nlm.nih.gov/pubmed/35073343
http://dx.doi.org/10.1371/journal.pone.0262806
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