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Carbon flux through photosynthesis and central carbon metabolism show distinct patterns between algae, C(3) and C(4) plants

Photosynthesis-related pathways are regarded as a promising avenue for crop improvement. Whilst empirical studies have shown that photosynthetic efficiency is higher in microalgae than in C(3) or C(4) crops, the underlying reasons remain unclear. Using a tailor-made microfluidics labelling system to...

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Autores principales: Treves, Haim, Küken, Anika, Arrivault, Stéphanie, Ishihara, Hirofumi, Hoppe, Ines, Erban, Alexander, Höhne, Melanie, Moraes, Thiago Alexandre, Kopka, Joachim, Szymanski, Jedrzej, Nikoloski, Zoran, Stitt, Mark
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8786664/
https://www.ncbi.nlm.nih.gov/pubmed/34949804
http://dx.doi.org/10.1038/s41477-021-01042-5
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author Treves, Haim
Küken, Anika
Arrivault, Stéphanie
Ishihara, Hirofumi
Hoppe, Ines
Erban, Alexander
Höhne, Melanie
Moraes, Thiago Alexandre
Kopka, Joachim
Szymanski, Jedrzej
Nikoloski, Zoran
Stitt, Mark
author_facet Treves, Haim
Küken, Anika
Arrivault, Stéphanie
Ishihara, Hirofumi
Hoppe, Ines
Erban, Alexander
Höhne, Melanie
Moraes, Thiago Alexandre
Kopka, Joachim
Szymanski, Jedrzej
Nikoloski, Zoran
Stitt, Mark
author_sort Treves, Haim
collection PubMed
description Photosynthesis-related pathways are regarded as a promising avenue for crop improvement. Whilst empirical studies have shown that photosynthetic efficiency is higher in microalgae than in C(3) or C(4) crops, the underlying reasons remain unclear. Using a tailor-made microfluidics labelling system to supply (13)CO(2) at steady state, we investigated in vivo labelling kinetics in intermediates of the Calvin Benson cycle and sugar, starch, organic acid and amino acid synthesis pathways, and in protein and lipids, in Chlamydomonas reinhardtii, Chlorella sorokiniana and Chlorella ohadii, which is the fastest growing green alga on record. We estimated flux patterns in these algae and compared them with published and new data from C(3) and C(4) plants. Our analyses identify distinct flux patterns supporting faster growth in photosynthetic cells, with some of the algae exhibiting faster ribulose 1,5-bisphosphate regeneration and increased fluxes through the lower glycolysis and anaplerotic pathways towards the tricarboxylic acid cycle, amino acid synthesis and lipid synthesis than in higher plants.
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spelling pubmed-87866642022-02-04 Carbon flux through photosynthesis and central carbon metabolism show distinct patterns between algae, C(3) and C(4) plants Treves, Haim Küken, Anika Arrivault, Stéphanie Ishihara, Hirofumi Hoppe, Ines Erban, Alexander Höhne, Melanie Moraes, Thiago Alexandre Kopka, Joachim Szymanski, Jedrzej Nikoloski, Zoran Stitt, Mark Nat Plants Article Photosynthesis-related pathways are regarded as a promising avenue for crop improvement. Whilst empirical studies have shown that photosynthetic efficiency is higher in microalgae than in C(3) or C(4) crops, the underlying reasons remain unclear. Using a tailor-made microfluidics labelling system to supply (13)CO(2) at steady state, we investigated in vivo labelling kinetics in intermediates of the Calvin Benson cycle and sugar, starch, organic acid and amino acid synthesis pathways, and in protein and lipids, in Chlamydomonas reinhardtii, Chlorella sorokiniana and Chlorella ohadii, which is the fastest growing green alga on record. We estimated flux patterns in these algae and compared them with published and new data from C(3) and C(4) plants. Our analyses identify distinct flux patterns supporting faster growth in photosynthetic cells, with some of the algae exhibiting faster ribulose 1,5-bisphosphate regeneration and increased fluxes through the lower glycolysis and anaplerotic pathways towards the tricarboxylic acid cycle, amino acid synthesis and lipid synthesis than in higher plants. Nature Publishing Group UK 2021-12-23 2022 /pmc/articles/PMC8786664/ /pubmed/34949804 http://dx.doi.org/10.1038/s41477-021-01042-5 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Treves, Haim
Küken, Anika
Arrivault, Stéphanie
Ishihara, Hirofumi
Hoppe, Ines
Erban, Alexander
Höhne, Melanie
Moraes, Thiago Alexandre
Kopka, Joachim
Szymanski, Jedrzej
Nikoloski, Zoran
Stitt, Mark
Carbon flux through photosynthesis and central carbon metabolism show distinct patterns between algae, C(3) and C(4) plants
title Carbon flux through photosynthesis and central carbon metabolism show distinct patterns between algae, C(3) and C(4) plants
title_full Carbon flux through photosynthesis and central carbon metabolism show distinct patterns between algae, C(3) and C(4) plants
title_fullStr Carbon flux through photosynthesis and central carbon metabolism show distinct patterns between algae, C(3) and C(4) plants
title_full_unstemmed Carbon flux through photosynthesis and central carbon metabolism show distinct patterns between algae, C(3) and C(4) plants
title_short Carbon flux through photosynthesis and central carbon metabolism show distinct patterns between algae, C(3) and C(4) plants
title_sort carbon flux through photosynthesis and central carbon metabolism show distinct patterns between algae, c(3) and c(4) plants
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8786664/
https://www.ncbi.nlm.nih.gov/pubmed/34949804
http://dx.doi.org/10.1038/s41477-021-01042-5
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