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Chitosan-collagen-hydroxyapatite membranes for tissue engineering
Tissue engineering is growing in developing new technologies focused on providing effective solutions to degenerative pathologies that affect different types of connective tissues. The search for biocompatible, bioactive, biodegradable, and multifunctional materials has grown significantly in recent...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer US
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8786760/ https://www.ncbi.nlm.nih.gov/pubmed/35072812 http://dx.doi.org/10.1007/s10856-022-06643-w |
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author | Becerra, José Rodriguez, Mariano Leal, Dayana Noris-Suarez, Karem Gonzalez, Gema |
author_facet | Becerra, José Rodriguez, Mariano Leal, Dayana Noris-Suarez, Karem Gonzalez, Gema |
author_sort | Becerra, José |
collection | PubMed |
description | Tissue engineering is growing in developing new technologies focused on providing effective solutions to degenerative pathologies that affect different types of connective tissues. The search for biocompatible, bioactive, biodegradable, and multifunctional materials has grown significantly in recent years. Chitosan, calcium phosphates collagen, and their combination as composite materials fulfill the required properties and could result in biostimulation for tissue regeneration. In the present work, the chitosan/collagen/hydroxyapatite membranes were prepared with different concentrations of collagen and hydroxyapatite. Cell adhesion was evaluated by MTS assay for two in vitro models. Additionally, cytotoxicity of the different membranes employing hemolysis of erythrocytes isolated from human blood was carried out. The structure of the membranes was analyzed by X-rays diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and thermal stability properties by thermogravimetric methods (TGA). The highest cell adhesion after 48 h was obtained for chitosan membranes with the highest hydroxyapatite and collagen content. All composite membranes showed good cell adhesion and low cytotoxicity, suggesting that these materials have a significant potential to be used as biomaterials for tissue engineering. [Figure: see text] |
format | Online Article Text |
id | pubmed-8786760 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer US |
record_format | MEDLINE/PubMed |
spelling | pubmed-87867602022-02-02 Chitosan-collagen-hydroxyapatite membranes for tissue engineering Becerra, José Rodriguez, Mariano Leal, Dayana Noris-Suarez, Karem Gonzalez, Gema J Mater Sci Mater Med Tissue Engineering Constructs and Cell Substrates Tissue engineering is growing in developing new technologies focused on providing effective solutions to degenerative pathologies that affect different types of connective tissues. The search for biocompatible, bioactive, biodegradable, and multifunctional materials has grown significantly in recent years. Chitosan, calcium phosphates collagen, and their combination as composite materials fulfill the required properties and could result in biostimulation for tissue regeneration. In the present work, the chitosan/collagen/hydroxyapatite membranes were prepared with different concentrations of collagen and hydroxyapatite. Cell adhesion was evaluated by MTS assay for two in vitro models. Additionally, cytotoxicity of the different membranes employing hemolysis of erythrocytes isolated from human blood was carried out. The structure of the membranes was analyzed by X-rays diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR), scanning electron microscopy (SEM), and thermal stability properties by thermogravimetric methods (TGA). The highest cell adhesion after 48 h was obtained for chitosan membranes with the highest hydroxyapatite and collagen content. All composite membranes showed good cell adhesion and low cytotoxicity, suggesting that these materials have a significant potential to be used as biomaterials for tissue engineering. [Figure: see text] Springer US 2022-01-24 2022 /pmc/articles/PMC8786760/ /pubmed/35072812 http://dx.doi.org/10.1007/s10856-022-06643-w Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Tissue Engineering Constructs and Cell Substrates Becerra, José Rodriguez, Mariano Leal, Dayana Noris-Suarez, Karem Gonzalez, Gema Chitosan-collagen-hydroxyapatite membranes for tissue engineering |
title | Chitosan-collagen-hydroxyapatite membranes for tissue engineering |
title_full | Chitosan-collagen-hydroxyapatite membranes for tissue engineering |
title_fullStr | Chitosan-collagen-hydroxyapatite membranes for tissue engineering |
title_full_unstemmed | Chitosan-collagen-hydroxyapatite membranes for tissue engineering |
title_short | Chitosan-collagen-hydroxyapatite membranes for tissue engineering |
title_sort | chitosan-collagen-hydroxyapatite membranes for tissue engineering |
topic | Tissue Engineering Constructs and Cell Substrates |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8786760/ https://www.ncbi.nlm.nih.gov/pubmed/35072812 http://dx.doi.org/10.1007/s10856-022-06643-w |
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