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Molecular Characterization Revealed the Role of Thaumatin-Like Proteins of Bread Wheat in Stress Response

Thaumatin-like proteins (TLPs) are related to pathogenesis-related-5 (PR-5) family and involved in stress response. Herein, a total of 93 TLP genes were identified in the genome of Triticum aestivum. Further, we identified 26, 27, 39, and 37 TLP genes in the Brachypodium distachyon, Oryza sativa, So...

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Autores principales: Sharma, Alok, Sharma, Himanshu, Rajput, Ruchika, Pandey, Ashutosh, Upadhyay, Santosh Kumar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8786798/
https://www.ncbi.nlm.nih.gov/pubmed/35087559
http://dx.doi.org/10.3389/fpls.2021.807448
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author Sharma, Alok
Sharma, Himanshu
Rajput, Ruchika
Pandey, Ashutosh
Upadhyay, Santosh Kumar
author_facet Sharma, Alok
Sharma, Himanshu
Rajput, Ruchika
Pandey, Ashutosh
Upadhyay, Santosh Kumar
author_sort Sharma, Alok
collection PubMed
description Thaumatin-like proteins (TLPs) are related to pathogenesis-related-5 (PR-5) family and involved in stress response. Herein, a total of 93 TLP genes were identified in the genome of Triticum aestivum. Further, we identified 26, 27, 39, and 37 TLP genes in the Brachypodium distachyon, Oryza sativa, Sorghum bicolor, and Zea mays genomes for comparative characterization, respectively. They could be grouped into small and long TLPs with conserved thaumatin signature motif. Tightly clustered genes exhibited conserved gene and protein structure. The physicochemical analyses suggested significant differences between small and long TLPs. Evolutionary analyses suggested the role of duplication events and purifying selection in the expansion of the TLP gene family. Expression analyses revealed the possible roles of TLPs in plant development and abiotic and fungal stress response. Recombinant expression of TaTLP2-B in Saccharomyces cerevisiae provided significant tolerance against cold, heat, osmotic, and salt stresses. The results depicted the importance of TLPs in cereal crops that would be highly useful in future crop improvement programs.
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spelling pubmed-87867982022-01-26 Molecular Characterization Revealed the Role of Thaumatin-Like Proteins of Bread Wheat in Stress Response Sharma, Alok Sharma, Himanshu Rajput, Ruchika Pandey, Ashutosh Upadhyay, Santosh Kumar Front Plant Sci Plant Science Thaumatin-like proteins (TLPs) are related to pathogenesis-related-5 (PR-5) family and involved in stress response. Herein, a total of 93 TLP genes were identified in the genome of Triticum aestivum. Further, we identified 26, 27, 39, and 37 TLP genes in the Brachypodium distachyon, Oryza sativa, Sorghum bicolor, and Zea mays genomes for comparative characterization, respectively. They could be grouped into small and long TLPs with conserved thaumatin signature motif. Tightly clustered genes exhibited conserved gene and protein structure. The physicochemical analyses suggested significant differences between small and long TLPs. Evolutionary analyses suggested the role of duplication events and purifying selection in the expansion of the TLP gene family. Expression analyses revealed the possible roles of TLPs in plant development and abiotic and fungal stress response. Recombinant expression of TaTLP2-B in Saccharomyces cerevisiae provided significant tolerance against cold, heat, osmotic, and salt stresses. The results depicted the importance of TLPs in cereal crops that would be highly useful in future crop improvement programs. Frontiers Media S.A. 2022-01-11 /pmc/articles/PMC8786798/ /pubmed/35087559 http://dx.doi.org/10.3389/fpls.2021.807448 Text en Copyright © 2022 Sharma, Sharma, Rajput, Pandey and Upadhyay. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Sharma, Alok
Sharma, Himanshu
Rajput, Ruchika
Pandey, Ashutosh
Upadhyay, Santosh Kumar
Molecular Characterization Revealed the Role of Thaumatin-Like Proteins of Bread Wheat in Stress Response
title Molecular Characterization Revealed the Role of Thaumatin-Like Proteins of Bread Wheat in Stress Response
title_full Molecular Characterization Revealed the Role of Thaumatin-Like Proteins of Bread Wheat in Stress Response
title_fullStr Molecular Characterization Revealed the Role of Thaumatin-Like Proteins of Bread Wheat in Stress Response
title_full_unstemmed Molecular Characterization Revealed the Role of Thaumatin-Like Proteins of Bread Wheat in Stress Response
title_short Molecular Characterization Revealed the Role of Thaumatin-Like Proteins of Bread Wheat in Stress Response
title_sort molecular characterization revealed the role of thaumatin-like proteins of bread wheat in stress response
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8786798/
https://www.ncbi.nlm.nih.gov/pubmed/35087559
http://dx.doi.org/10.3389/fpls.2021.807448
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