Cargando…
The role of TolA, TolB, and TolR in cell morphology, OMVs production, and virulence of Salmonella Choleraesuis
The Tol–Pal system of Gram-negative bacteria is necessary for maintaining outer membrane integrity. It is a multiprotein complex of five envelope proteins, TolQ, TolR, TolA, TolB, and Pal. These proteins were first investigated in E. coli, and subsequently been identified in many other bacterial gen...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Springer Berlin Heidelberg
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8787014/ https://www.ncbi.nlm.nih.gov/pubmed/35075554 http://dx.doi.org/10.1186/s13568-022-01347-4 |
_version_ | 1784639266455814144 |
---|---|
author | Li, Quan Li, Zheng Fei, Xia Tian, Yichen Zhou, Guodong Hu, Yuhan Wang, Shifeng Shi, Huoying |
author_facet | Li, Quan Li, Zheng Fei, Xia Tian, Yichen Zhou, Guodong Hu, Yuhan Wang, Shifeng Shi, Huoying |
author_sort | Li, Quan |
collection | PubMed |
description | The Tol–Pal system of Gram-negative bacteria is necessary for maintaining outer membrane integrity. It is a multiprotein complex of five envelope proteins, TolQ, TolR, TolA, TolB, and Pal. These proteins were first investigated in E. coli, and subsequently been identified in many other bacterial genera. However, the function of the Tol–Pal system in Salmonella Choleraesuis pathogenesis is still unclear. Here, we reported the role of three of these proteins in the phenotype and biology of S. Choleraesuis. We found that mutations in tolA, tolB, and tolR caused severe damage to the cell wall, which was supported by observing the microstructure of spherical forms, long chains, flagella defects, and membrane blebbing. We confirmed that all the mutants significantly decreased S. Choleraesuis survival when exposed to sodium deoxycholate and exhibited a high sensitivity to vancomycin, which may be explained by the disruption of envelope integrity. In addition, tolA, tolB, and tolR mutants displayed attenuated virulence in a mouse infection model. This could be interpreted as a series of defective phenotypes in the mutants, such as severe defects in envelope integrity, growth, and motility. Further investigation showed that all the genes participate in outer membrane vesicles (OMVs) biogenesis. Interestingly, immunization with OMVs from ΔtolB efficiently enhanced murine viability in contrast to OMVs from the wild-type S. Choleraesuis, suggesting its potential use in vaccination strategies. Collectively, this study provides an insight into the biological role of the S. Choleraesuis Tol–Pal system. |
format | Online Article Text |
id | pubmed-8787014 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Springer Berlin Heidelberg |
record_format | MEDLINE/PubMed |
spelling | pubmed-87870142022-02-02 The role of TolA, TolB, and TolR in cell morphology, OMVs production, and virulence of Salmonella Choleraesuis Li, Quan Li, Zheng Fei, Xia Tian, Yichen Zhou, Guodong Hu, Yuhan Wang, Shifeng Shi, Huoying AMB Express Original Article The Tol–Pal system of Gram-negative bacteria is necessary for maintaining outer membrane integrity. It is a multiprotein complex of five envelope proteins, TolQ, TolR, TolA, TolB, and Pal. These proteins were first investigated in E. coli, and subsequently been identified in many other bacterial genera. However, the function of the Tol–Pal system in Salmonella Choleraesuis pathogenesis is still unclear. Here, we reported the role of three of these proteins in the phenotype and biology of S. Choleraesuis. We found that mutations in tolA, tolB, and tolR caused severe damage to the cell wall, which was supported by observing the microstructure of spherical forms, long chains, flagella defects, and membrane blebbing. We confirmed that all the mutants significantly decreased S. Choleraesuis survival when exposed to sodium deoxycholate and exhibited a high sensitivity to vancomycin, which may be explained by the disruption of envelope integrity. In addition, tolA, tolB, and tolR mutants displayed attenuated virulence in a mouse infection model. This could be interpreted as a series of defective phenotypes in the mutants, such as severe defects in envelope integrity, growth, and motility. Further investigation showed that all the genes participate in outer membrane vesicles (OMVs) biogenesis. Interestingly, immunization with OMVs from ΔtolB efficiently enhanced murine viability in contrast to OMVs from the wild-type S. Choleraesuis, suggesting its potential use in vaccination strategies. Collectively, this study provides an insight into the biological role of the S. Choleraesuis Tol–Pal system. Springer Berlin Heidelberg 2022-01-25 /pmc/articles/PMC8787014/ /pubmed/35075554 http://dx.doi.org/10.1186/s13568-022-01347-4 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Original Article Li, Quan Li, Zheng Fei, Xia Tian, Yichen Zhou, Guodong Hu, Yuhan Wang, Shifeng Shi, Huoying The role of TolA, TolB, and TolR in cell morphology, OMVs production, and virulence of Salmonella Choleraesuis |
title | The role of TolA, TolB, and TolR in cell morphology, OMVs production, and virulence of Salmonella Choleraesuis |
title_full | The role of TolA, TolB, and TolR in cell morphology, OMVs production, and virulence of Salmonella Choleraesuis |
title_fullStr | The role of TolA, TolB, and TolR in cell morphology, OMVs production, and virulence of Salmonella Choleraesuis |
title_full_unstemmed | The role of TolA, TolB, and TolR in cell morphology, OMVs production, and virulence of Salmonella Choleraesuis |
title_short | The role of TolA, TolB, and TolR in cell morphology, OMVs production, and virulence of Salmonella Choleraesuis |
title_sort | role of tola, tolb, and tolr in cell morphology, omvs production, and virulence of salmonella choleraesuis |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8787014/ https://www.ncbi.nlm.nih.gov/pubmed/35075554 http://dx.doi.org/10.1186/s13568-022-01347-4 |
work_keys_str_mv | AT liquan theroleoftolatolbandtolrincellmorphologyomvsproductionandvirulenceofsalmonellacholeraesuis AT lizheng theroleoftolatolbandtolrincellmorphologyomvsproductionandvirulenceofsalmonellacholeraesuis AT feixia theroleoftolatolbandtolrincellmorphologyomvsproductionandvirulenceofsalmonellacholeraesuis AT tianyichen theroleoftolatolbandtolrincellmorphologyomvsproductionandvirulenceofsalmonellacholeraesuis AT zhouguodong theroleoftolatolbandtolrincellmorphologyomvsproductionandvirulenceofsalmonellacholeraesuis AT huyuhan theroleoftolatolbandtolrincellmorphologyomvsproductionandvirulenceofsalmonellacholeraesuis AT wangshifeng theroleoftolatolbandtolrincellmorphologyomvsproductionandvirulenceofsalmonellacholeraesuis AT shihuoying theroleoftolatolbandtolrincellmorphologyomvsproductionandvirulenceofsalmonellacholeraesuis AT liquan roleoftolatolbandtolrincellmorphologyomvsproductionandvirulenceofsalmonellacholeraesuis AT lizheng roleoftolatolbandtolrincellmorphologyomvsproductionandvirulenceofsalmonellacholeraesuis AT feixia roleoftolatolbandtolrincellmorphologyomvsproductionandvirulenceofsalmonellacholeraesuis AT tianyichen roleoftolatolbandtolrincellmorphologyomvsproductionandvirulenceofsalmonellacholeraesuis AT zhouguodong roleoftolatolbandtolrincellmorphologyomvsproductionandvirulenceofsalmonellacholeraesuis AT huyuhan roleoftolatolbandtolrincellmorphologyomvsproductionandvirulenceofsalmonellacholeraesuis AT wangshifeng roleoftolatolbandtolrincellmorphologyomvsproductionandvirulenceofsalmonellacholeraesuis AT shihuoying roleoftolatolbandtolrincellmorphologyomvsproductionandvirulenceofsalmonellacholeraesuis |