Cargando…

l-Isoleucine Administration Alleviates DSS-Induced Colitis by Regulating TLR4/MyD88/NF-κB Pathway in Rats

Inflammatory bowel disease (namely, colitis) severely impairs human health. Isoleucine is reported to regulate immune function (such as the production of immunoreactive substances). The aim of this study was to investigate whether l-isoleucine administration might alleviate dextran sulfate sodium (D...

Descripción completa

Detalles Bibliográficos
Autores principales: Mao, Xiangbing, Sun, Rui, Wang, Qingxiang, Chen, Daiwen, Yu, Bing, He, Jun, Yu, Jie, Luo, Junqiu, Luo, Yuheng, Yan, Hui, Wang, Jianping, Wang, Huifen, Wang, Quyuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8787224/
https://www.ncbi.nlm.nih.gov/pubmed/35087537
http://dx.doi.org/10.3389/fimmu.2021.817583
_version_ 1784639314494226432
author Mao, Xiangbing
Sun, Rui
Wang, Qingxiang
Chen, Daiwen
Yu, Bing
He, Jun
Yu, Jie
Luo, Junqiu
Luo, Yuheng
Yan, Hui
Wang, Jianping
Wang, Huifen
Wang, Quyuan
author_facet Mao, Xiangbing
Sun, Rui
Wang, Qingxiang
Chen, Daiwen
Yu, Bing
He, Jun
Yu, Jie
Luo, Junqiu
Luo, Yuheng
Yan, Hui
Wang, Jianping
Wang, Huifen
Wang, Quyuan
author_sort Mao, Xiangbing
collection PubMed
description Inflammatory bowel disease (namely, colitis) severely impairs human health. Isoleucine is reported to regulate immune function (such as the production of immunoreactive substances). The aim of this study was to investigate whether l-isoleucine administration might alleviate dextran sulfate sodium (DSS)-induced colitis in rats. In the in vitro trial, IEC-18 cells were treated by 4 mmol/L l-isoleucine for 12 h, which relieved the decrease of cell viability that was induced by TNF-α (10 ng/ml) challenge for 24 h (P <0.05). Then, in the in vivo experiment, a total of 44 Wistar rats were allotted into 2 groups that were fed l-isoleucine-supplemented diet and control diet for 35 d. From 15 to 35 d, half of the rats in the 2 groups drank the 4% DSS-adding water. Average daily gain, average daily feed intake and feed conversion of rats were impaired by DSS challenge (P <0.05). Drinking the DSS-supplementing water also increased disease activity index (DAI) and serum urea nitrogen level (P <0.05), shortened colonic length (P <0.05), impaired colonic enterocyte apoptosis, cell cycle, and the ZO-1 mRNA expression (P <0.05), increased the ratio of CD11c-, CD64-, and CD169-positive cells in colon (P <0.05), and induced extensive ulcer, infiltration of inflammatory cells, and collagenous fiber hyperplasia in colon. However, dietary l-isoleucine supplementation attenuated the negative effect of DSS challenge on growth performance (P <0.05), DAI (P <0.05), colonic length and enterocyte apoptosis (P <0.05), and dysfunction of colonic histology, and downregulated the ratio of CD11c-, CD64-, and CD169-positive cells, pro-inflammation cytokines and the mRNA expression of TLR4, MyD88, and NF-κB in the colon of rats (P <0.05). These results suggest that supplementing l-isoleucine in diet improved the DSS-induced growth stunting and colonic damage in rats, which could be associated with the downregulation of inflammation via regulating TLR4/MyD88/NF-κB pathway in colon.
format Online
Article
Text
id pubmed-8787224
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-87872242022-01-26 l-Isoleucine Administration Alleviates DSS-Induced Colitis by Regulating TLR4/MyD88/NF-κB Pathway in Rats Mao, Xiangbing Sun, Rui Wang, Qingxiang Chen, Daiwen Yu, Bing He, Jun Yu, Jie Luo, Junqiu Luo, Yuheng Yan, Hui Wang, Jianping Wang, Huifen Wang, Quyuan Front Immunol Immunology Inflammatory bowel disease (namely, colitis) severely impairs human health. Isoleucine is reported to regulate immune function (such as the production of immunoreactive substances). The aim of this study was to investigate whether l-isoleucine administration might alleviate dextran sulfate sodium (DSS)-induced colitis in rats. In the in vitro trial, IEC-18 cells were treated by 4 mmol/L l-isoleucine for 12 h, which relieved the decrease of cell viability that was induced by TNF-α (10 ng/ml) challenge for 24 h (P <0.05). Then, in the in vivo experiment, a total of 44 Wistar rats were allotted into 2 groups that were fed l-isoleucine-supplemented diet and control diet for 35 d. From 15 to 35 d, half of the rats in the 2 groups drank the 4% DSS-adding water. Average daily gain, average daily feed intake and feed conversion of rats were impaired by DSS challenge (P <0.05). Drinking the DSS-supplementing water also increased disease activity index (DAI) and serum urea nitrogen level (P <0.05), shortened colonic length (P <0.05), impaired colonic enterocyte apoptosis, cell cycle, and the ZO-1 mRNA expression (P <0.05), increased the ratio of CD11c-, CD64-, and CD169-positive cells in colon (P <0.05), and induced extensive ulcer, infiltration of inflammatory cells, and collagenous fiber hyperplasia in colon. However, dietary l-isoleucine supplementation attenuated the negative effect of DSS challenge on growth performance (P <0.05), DAI (P <0.05), colonic length and enterocyte apoptosis (P <0.05), and dysfunction of colonic histology, and downregulated the ratio of CD11c-, CD64-, and CD169-positive cells, pro-inflammation cytokines and the mRNA expression of TLR4, MyD88, and NF-κB in the colon of rats (P <0.05). These results suggest that supplementing l-isoleucine in diet improved the DSS-induced growth stunting and colonic damage in rats, which could be associated with the downregulation of inflammation via regulating TLR4/MyD88/NF-κB pathway in colon. Frontiers Media S.A. 2022-01-11 /pmc/articles/PMC8787224/ /pubmed/35087537 http://dx.doi.org/10.3389/fimmu.2021.817583 Text en Copyright © 2022 Mao, Sun, Wang, Chen, Yu, He, Yu, Luo, Luo, Yan, Wang, Wang and Wang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Mao, Xiangbing
Sun, Rui
Wang, Qingxiang
Chen, Daiwen
Yu, Bing
He, Jun
Yu, Jie
Luo, Junqiu
Luo, Yuheng
Yan, Hui
Wang, Jianping
Wang, Huifen
Wang, Quyuan
l-Isoleucine Administration Alleviates DSS-Induced Colitis by Regulating TLR4/MyD88/NF-κB Pathway in Rats
title l-Isoleucine Administration Alleviates DSS-Induced Colitis by Regulating TLR4/MyD88/NF-κB Pathway in Rats
title_full l-Isoleucine Administration Alleviates DSS-Induced Colitis by Regulating TLR4/MyD88/NF-κB Pathway in Rats
title_fullStr l-Isoleucine Administration Alleviates DSS-Induced Colitis by Regulating TLR4/MyD88/NF-κB Pathway in Rats
title_full_unstemmed l-Isoleucine Administration Alleviates DSS-Induced Colitis by Regulating TLR4/MyD88/NF-κB Pathway in Rats
title_short l-Isoleucine Administration Alleviates DSS-Induced Colitis by Regulating TLR4/MyD88/NF-κB Pathway in Rats
title_sort l-isoleucine administration alleviates dss-induced colitis by regulating tlr4/myd88/nf-κb pathway in rats
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8787224/
https://www.ncbi.nlm.nih.gov/pubmed/35087537
http://dx.doi.org/10.3389/fimmu.2021.817583
work_keys_str_mv AT maoxiangbing lisoleucineadministrationalleviatesdssinducedcolitisbyregulatingtlr4myd88nfkbpathwayinrats
AT sunrui lisoleucineadministrationalleviatesdssinducedcolitisbyregulatingtlr4myd88nfkbpathwayinrats
AT wangqingxiang lisoleucineadministrationalleviatesdssinducedcolitisbyregulatingtlr4myd88nfkbpathwayinrats
AT chendaiwen lisoleucineadministrationalleviatesdssinducedcolitisbyregulatingtlr4myd88nfkbpathwayinrats
AT yubing lisoleucineadministrationalleviatesdssinducedcolitisbyregulatingtlr4myd88nfkbpathwayinrats
AT hejun lisoleucineadministrationalleviatesdssinducedcolitisbyregulatingtlr4myd88nfkbpathwayinrats
AT yujie lisoleucineadministrationalleviatesdssinducedcolitisbyregulatingtlr4myd88nfkbpathwayinrats
AT luojunqiu lisoleucineadministrationalleviatesdssinducedcolitisbyregulatingtlr4myd88nfkbpathwayinrats
AT luoyuheng lisoleucineadministrationalleviatesdssinducedcolitisbyregulatingtlr4myd88nfkbpathwayinrats
AT yanhui lisoleucineadministrationalleviatesdssinducedcolitisbyregulatingtlr4myd88nfkbpathwayinrats
AT wangjianping lisoleucineadministrationalleviatesdssinducedcolitisbyregulatingtlr4myd88nfkbpathwayinrats
AT wanghuifen lisoleucineadministrationalleviatesdssinducedcolitisbyregulatingtlr4myd88nfkbpathwayinrats
AT wangquyuan lisoleucineadministrationalleviatesdssinducedcolitisbyregulatingtlr4myd88nfkbpathwayinrats