Cargando…

Phosphoproteomics Reveals Regulation of Secondary Metabolites in Mahonia bealei Exposed to Ultraviolet-B Radiation

Mahonia bealei (M. bealei) is a traditional Chinese medicine containing a high alkaloid content used to treat various diseases. Generally, only dried root and stem are used as medicines, considering that the alkaloid content in M. bealei leaves is lower than in the stems and roots. Some previous res...

Descripción completa

Detalles Bibliográficos
Autores principales: Liu, Amin, Liu, Shengzhi, Li, Yaohan, Tao, Minglei, Han, Haote, Zhong, Zhuoheng, Zhu, Wei, Tian, Jingkui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8787227/
https://www.ncbi.nlm.nih.gov/pubmed/35087555
http://dx.doi.org/10.3389/fpls.2021.794906
_version_ 1784639315208306688
author Liu, Amin
Liu, Shengzhi
Li, Yaohan
Tao, Minglei
Han, Haote
Zhong, Zhuoheng
Zhu, Wei
Tian, Jingkui
author_facet Liu, Amin
Liu, Shengzhi
Li, Yaohan
Tao, Minglei
Han, Haote
Zhong, Zhuoheng
Zhu, Wei
Tian, Jingkui
author_sort Liu, Amin
collection PubMed
description Mahonia bealei (M. bealei) is a traditional Chinese medicine containing a high alkaloid content used to treat various diseases. Generally, only dried root and stem are used as medicines, considering that the alkaloid content in M. bealei leaves is lower than in the stems and roots. Some previous research found that alkaloid and flavonoid contents in the M. bealei leaves may increase when exposed to ultraviolet B (UV-B) radiation. However, the underlying mechanism of action is still unclear. In this study, we used titanium dioxide material enrichment and mass-based label-free quantitative proteomics techniques to explore the effect and mechanism of M. bealei leaves when exposed to UV-B treatment. Our data suggest that UV-B radiation increases the ATP content, photosynthetic pigment content, and some enzymatic/nonenzymatic indicators in the leaves of M. bealei. Moreover, phosphoproteomics suggests phosphoproteins related to mitogen-activated protein kinase (MAPK) signal transduction and the plant hormone brassinosteroid signaling pathway as well as phosphoproteins related to photosynthesis, glycolysis, the tricarboxylic acid cycle, and the amino acid synthesis/metabolism pathway are all affected by UV-B radiation. These results suggest that the UV-B radiation activates the oxidative stress response, MAPK signal transduction pathway, and photosynthetic energy metabolism pathway, which may lead to the accumulation of secondary metabolites in M. bealei leaves.
format Online
Article
Text
id pubmed-8787227
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-87872272022-01-26 Phosphoproteomics Reveals Regulation of Secondary Metabolites in Mahonia bealei Exposed to Ultraviolet-B Radiation Liu, Amin Liu, Shengzhi Li, Yaohan Tao, Minglei Han, Haote Zhong, Zhuoheng Zhu, Wei Tian, Jingkui Front Plant Sci Plant Science Mahonia bealei (M. bealei) is a traditional Chinese medicine containing a high alkaloid content used to treat various diseases. Generally, only dried root and stem are used as medicines, considering that the alkaloid content in M. bealei leaves is lower than in the stems and roots. Some previous research found that alkaloid and flavonoid contents in the M. bealei leaves may increase when exposed to ultraviolet B (UV-B) radiation. However, the underlying mechanism of action is still unclear. In this study, we used titanium dioxide material enrichment and mass-based label-free quantitative proteomics techniques to explore the effect and mechanism of M. bealei leaves when exposed to UV-B treatment. Our data suggest that UV-B radiation increases the ATP content, photosynthetic pigment content, and some enzymatic/nonenzymatic indicators in the leaves of M. bealei. Moreover, phosphoproteomics suggests phosphoproteins related to mitogen-activated protein kinase (MAPK) signal transduction and the plant hormone brassinosteroid signaling pathway as well as phosphoproteins related to photosynthesis, glycolysis, the tricarboxylic acid cycle, and the amino acid synthesis/metabolism pathway are all affected by UV-B radiation. These results suggest that the UV-B radiation activates the oxidative stress response, MAPK signal transduction pathway, and photosynthetic energy metabolism pathway, which may lead to the accumulation of secondary metabolites in M. bealei leaves. Frontiers Media S.A. 2022-01-11 /pmc/articles/PMC8787227/ /pubmed/35087555 http://dx.doi.org/10.3389/fpls.2021.794906 Text en Copyright © 2022 Liu, Liu, Li, Tao, Han, Zhong, Zhu and Tian. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Liu, Amin
Liu, Shengzhi
Li, Yaohan
Tao, Minglei
Han, Haote
Zhong, Zhuoheng
Zhu, Wei
Tian, Jingkui
Phosphoproteomics Reveals Regulation of Secondary Metabolites in Mahonia bealei Exposed to Ultraviolet-B Radiation
title Phosphoproteomics Reveals Regulation of Secondary Metabolites in Mahonia bealei Exposed to Ultraviolet-B Radiation
title_full Phosphoproteomics Reveals Regulation of Secondary Metabolites in Mahonia bealei Exposed to Ultraviolet-B Radiation
title_fullStr Phosphoproteomics Reveals Regulation of Secondary Metabolites in Mahonia bealei Exposed to Ultraviolet-B Radiation
title_full_unstemmed Phosphoproteomics Reveals Regulation of Secondary Metabolites in Mahonia bealei Exposed to Ultraviolet-B Radiation
title_short Phosphoproteomics Reveals Regulation of Secondary Metabolites in Mahonia bealei Exposed to Ultraviolet-B Radiation
title_sort phosphoproteomics reveals regulation of secondary metabolites in mahonia bealei exposed to ultraviolet-b radiation
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8787227/
https://www.ncbi.nlm.nih.gov/pubmed/35087555
http://dx.doi.org/10.3389/fpls.2021.794906
work_keys_str_mv AT liuamin phosphoproteomicsrevealsregulationofsecondarymetabolitesinmahoniabealeiexposedtoultravioletbradiation
AT liushengzhi phosphoproteomicsrevealsregulationofsecondarymetabolitesinmahoniabealeiexposedtoultravioletbradiation
AT liyaohan phosphoproteomicsrevealsregulationofsecondarymetabolitesinmahoniabealeiexposedtoultravioletbradiation
AT taominglei phosphoproteomicsrevealsregulationofsecondarymetabolitesinmahoniabealeiexposedtoultravioletbradiation
AT hanhaote phosphoproteomicsrevealsregulationofsecondarymetabolitesinmahoniabealeiexposedtoultravioletbradiation
AT zhongzhuoheng phosphoproteomicsrevealsregulationofsecondarymetabolitesinmahoniabealeiexposedtoultravioletbradiation
AT zhuwei phosphoproteomicsrevealsregulationofsecondarymetabolitesinmahoniabealeiexposedtoultravioletbradiation
AT tianjingkui phosphoproteomicsrevealsregulationofsecondarymetabolitesinmahoniabealeiexposedtoultravioletbradiation