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Molecular Characterization of a Novel Budgerigar Fledgling Disease Virus Strain From Budgerigars in China

Budgerigar fledgling disease virus (BFDV) is the causative polyomavirus of budgerigar fledgling disease, an important avian immunosuppressive disease in budgerigars (Melopsittacus undulatus). In the current study, we explored the etiological role and molecular characteristics of BFDV. We identified...

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Autores principales: Hu, Xiaoliang, Cai, Dongdong, Liu, Siru, Li, Yan, Chen, Lulu, Luo, Guangmei, Pu, Hongli, He, Yucan, Liu, Xiangxiao, Zhao, Lili, Cao, Hongzhi, Yang, Tiankuo, Tian, Zhige
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8787288/
https://www.ncbi.nlm.nih.gov/pubmed/35087894
http://dx.doi.org/10.3389/fvets.2021.813397
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author Hu, Xiaoliang
Cai, Dongdong
Liu, Siru
Li, Yan
Chen, Lulu
Luo, Guangmei
Pu, Hongli
He, Yucan
Liu, Xiangxiao
Zhao, Lili
Cao, Hongzhi
Yang, Tiankuo
Tian, Zhige
author_facet Hu, Xiaoliang
Cai, Dongdong
Liu, Siru
Li, Yan
Chen, Lulu
Luo, Guangmei
Pu, Hongli
He, Yucan
Liu, Xiangxiao
Zhao, Lili
Cao, Hongzhi
Yang, Tiankuo
Tian, Zhige
author_sort Hu, Xiaoliang
collection PubMed
description Budgerigar fledgling disease virus (BFDV) is the causative polyomavirus of budgerigar fledgling disease, an important avian immunosuppressive disease in budgerigars (Melopsittacus undulatus). In the current study, we explored the etiological role and molecular characteristics of BFDV. We identified a novel BFDV strain, designated as SC-YB19, belonging to a unique cluster with three other domestic strains (WF-GM01, SD18, and APV-P) and closely related to Polish isolates based on complete sequences. Sequence analysis showed that SC-YB19 had an 18-nucleotide (nt) deletion in the enhancer region, corresponding to the sequence position 164–181 nt, which differed significantly from all other BFDV strains. Based on sequence alignment, three unique nucleotide substitutions were found in VP4 (position 821), VP1 (position 2,383), and T-antigen (position 3,517) of SC-YB19, compared with SD18, WF-GM01, QDJM01, HBYM02, APV7, and BFDV1. Phylogenetic analyses based on complete sequences suggested that SC-YB19, along with the domestic WF-GM01, SD18, and APV-P strains, formed a single branch and were closely related to Polish, Japanese, and American isolates. These results demonstrate that BFDV genotype variations are co-circulating in China, thus providing important insight into BFDV evolution.
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spelling pubmed-87872882022-01-26 Molecular Characterization of a Novel Budgerigar Fledgling Disease Virus Strain From Budgerigars in China Hu, Xiaoliang Cai, Dongdong Liu, Siru Li, Yan Chen, Lulu Luo, Guangmei Pu, Hongli He, Yucan Liu, Xiangxiao Zhao, Lili Cao, Hongzhi Yang, Tiankuo Tian, Zhige Front Vet Sci Veterinary Science Budgerigar fledgling disease virus (BFDV) is the causative polyomavirus of budgerigar fledgling disease, an important avian immunosuppressive disease in budgerigars (Melopsittacus undulatus). In the current study, we explored the etiological role and molecular characteristics of BFDV. We identified a novel BFDV strain, designated as SC-YB19, belonging to a unique cluster with three other domestic strains (WF-GM01, SD18, and APV-P) and closely related to Polish isolates based on complete sequences. Sequence analysis showed that SC-YB19 had an 18-nucleotide (nt) deletion in the enhancer region, corresponding to the sequence position 164–181 nt, which differed significantly from all other BFDV strains. Based on sequence alignment, three unique nucleotide substitutions were found in VP4 (position 821), VP1 (position 2,383), and T-antigen (position 3,517) of SC-YB19, compared with SD18, WF-GM01, QDJM01, HBYM02, APV7, and BFDV1. Phylogenetic analyses based on complete sequences suggested that SC-YB19, along with the domestic WF-GM01, SD18, and APV-P strains, formed a single branch and were closely related to Polish, Japanese, and American isolates. These results demonstrate that BFDV genotype variations are co-circulating in China, thus providing important insight into BFDV evolution. Frontiers Media S.A. 2022-01-11 /pmc/articles/PMC8787288/ /pubmed/35087894 http://dx.doi.org/10.3389/fvets.2021.813397 Text en Copyright © 2022 Hu, Cai, Liu, Li, Chen, Luo, Pu, He, Liu, Zhao, Cao, Yang and Tian. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Hu, Xiaoliang
Cai, Dongdong
Liu, Siru
Li, Yan
Chen, Lulu
Luo, Guangmei
Pu, Hongli
He, Yucan
Liu, Xiangxiao
Zhao, Lili
Cao, Hongzhi
Yang, Tiankuo
Tian, Zhige
Molecular Characterization of a Novel Budgerigar Fledgling Disease Virus Strain From Budgerigars in China
title Molecular Characterization of a Novel Budgerigar Fledgling Disease Virus Strain From Budgerigars in China
title_full Molecular Characterization of a Novel Budgerigar Fledgling Disease Virus Strain From Budgerigars in China
title_fullStr Molecular Characterization of a Novel Budgerigar Fledgling Disease Virus Strain From Budgerigars in China
title_full_unstemmed Molecular Characterization of a Novel Budgerigar Fledgling Disease Virus Strain From Budgerigars in China
title_short Molecular Characterization of a Novel Budgerigar Fledgling Disease Virus Strain From Budgerigars in China
title_sort molecular characterization of a novel budgerigar fledgling disease virus strain from budgerigars in china
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8787288/
https://www.ncbi.nlm.nih.gov/pubmed/35087894
http://dx.doi.org/10.3389/fvets.2021.813397
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