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Molecular Characterization of a Novel Budgerigar Fledgling Disease Virus Strain From Budgerigars in China
Budgerigar fledgling disease virus (BFDV) is the causative polyomavirus of budgerigar fledgling disease, an important avian immunosuppressive disease in budgerigars (Melopsittacus undulatus). In the current study, we explored the etiological role and molecular characteristics of BFDV. We identified...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8787288/ https://www.ncbi.nlm.nih.gov/pubmed/35087894 http://dx.doi.org/10.3389/fvets.2021.813397 |
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author | Hu, Xiaoliang Cai, Dongdong Liu, Siru Li, Yan Chen, Lulu Luo, Guangmei Pu, Hongli He, Yucan Liu, Xiangxiao Zhao, Lili Cao, Hongzhi Yang, Tiankuo Tian, Zhige |
author_facet | Hu, Xiaoliang Cai, Dongdong Liu, Siru Li, Yan Chen, Lulu Luo, Guangmei Pu, Hongli He, Yucan Liu, Xiangxiao Zhao, Lili Cao, Hongzhi Yang, Tiankuo Tian, Zhige |
author_sort | Hu, Xiaoliang |
collection | PubMed |
description | Budgerigar fledgling disease virus (BFDV) is the causative polyomavirus of budgerigar fledgling disease, an important avian immunosuppressive disease in budgerigars (Melopsittacus undulatus). In the current study, we explored the etiological role and molecular characteristics of BFDV. We identified a novel BFDV strain, designated as SC-YB19, belonging to a unique cluster with three other domestic strains (WF-GM01, SD18, and APV-P) and closely related to Polish isolates based on complete sequences. Sequence analysis showed that SC-YB19 had an 18-nucleotide (nt) deletion in the enhancer region, corresponding to the sequence position 164–181 nt, which differed significantly from all other BFDV strains. Based on sequence alignment, three unique nucleotide substitutions were found in VP4 (position 821), VP1 (position 2,383), and T-antigen (position 3,517) of SC-YB19, compared with SD18, WF-GM01, QDJM01, HBYM02, APV7, and BFDV1. Phylogenetic analyses based on complete sequences suggested that SC-YB19, along with the domestic WF-GM01, SD18, and APV-P strains, formed a single branch and were closely related to Polish, Japanese, and American isolates. These results demonstrate that BFDV genotype variations are co-circulating in China, thus providing important insight into BFDV evolution. |
format | Online Article Text |
id | pubmed-8787288 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-87872882022-01-26 Molecular Characterization of a Novel Budgerigar Fledgling Disease Virus Strain From Budgerigars in China Hu, Xiaoliang Cai, Dongdong Liu, Siru Li, Yan Chen, Lulu Luo, Guangmei Pu, Hongli He, Yucan Liu, Xiangxiao Zhao, Lili Cao, Hongzhi Yang, Tiankuo Tian, Zhige Front Vet Sci Veterinary Science Budgerigar fledgling disease virus (BFDV) is the causative polyomavirus of budgerigar fledgling disease, an important avian immunosuppressive disease in budgerigars (Melopsittacus undulatus). In the current study, we explored the etiological role and molecular characteristics of BFDV. We identified a novel BFDV strain, designated as SC-YB19, belonging to a unique cluster with three other domestic strains (WF-GM01, SD18, and APV-P) and closely related to Polish isolates based on complete sequences. Sequence analysis showed that SC-YB19 had an 18-nucleotide (nt) deletion in the enhancer region, corresponding to the sequence position 164–181 nt, which differed significantly from all other BFDV strains. Based on sequence alignment, three unique nucleotide substitutions were found in VP4 (position 821), VP1 (position 2,383), and T-antigen (position 3,517) of SC-YB19, compared with SD18, WF-GM01, QDJM01, HBYM02, APV7, and BFDV1. Phylogenetic analyses based on complete sequences suggested that SC-YB19, along with the domestic WF-GM01, SD18, and APV-P strains, formed a single branch and were closely related to Polish, Japanese, and American isolates. These results demonstrate that BFDV genotype variations are co-circulating in China, thus providing important insight into BFDV evolution. Frontiers Media S.A. 2022-01-11 /pmc/articles/PMC8787288/ /pubmed/35087894 http://dx.doi.org/10.3389/fvets.2021.813397 Text en Copyright © 2022 Hu, Cai, Liu, Li, Chen, Luo, Pu, He, Liu, Zhao, Cao, Yang and Tian. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Veterinary Science Hu, Xiaoliang Cai, Dongdong Liu, Siru Li, Yan Chen, Lulu Luo, Guangmei Pu, Hongli He, Yucan Liu, Xiangxiao Zhao, Lili Cao, Hongzhi Yang, Tiankuo Tian, Zhige Molecular Characterization of a Novel Budgerigar Fledgling Disease Virus Strain From Budgerigars in China |
title | Molecular Characterization of a Novel Budgerigar Fledgling Disease Virus Strain From Budgerigars in China |
title_full | Molecular Characterization of a Novel Budgerigar Fledgling Disease Virus Strain From Budgerigars in China |
title_fullStr | Molecular Characterization of a Novel Budgerigar Fledgling Disease Virus Strain From Budgerigars in China |
title_full_unstemmed | Molecular Characterization of a Novel Budgerigar Fledgling Disease Virus Strain From Budgerigars in China |
title_short | Molecular Characterization of a Novel Budgerigar Fledgling Disease Virus Strain From Budgerigars in China |
title_sort | molecular characterization of a novel budgerigar fledgling disease virus strain from budgerigars in china |
topic | Veterinary Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8787288/ https://www.ncbi.nlm.nih.gov/pubmed/35087894 http://dx.doi.org/10.3389/fvets.2021.813397 |
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