Enrichment of gut microbiome strains for cultivation-free genome sequencing using droplet microfluidics

We report a droplet microfluidic method to target and sort individual cells directly from complex microbiome samples and to prepare these cells for bulk whole-genome sequencing without cultivation. We characterize this approach by recovering bacteria spiked into human stool samples at a ratio as low...

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Detalles Bibliográficos
Autores principales: Pryszlak, Anna, Wenzel, Tobias, Seitz, Kiley West, Hildebrand, Falk, Kartal, Ece, Cosenza, Marco Raffaele, Benes, Vladimir, Bork, Peer, Merten, Christoph A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8787643/
https://www.ncbi.nlm.nih.gov/pubmed/35118437
http://dx.doi.org/10.1016/j.crmeth.2021.100137

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8787643/
https://www.ncbi.nlm.nih.gov/pubmed/35118437
http://dx.doi.org/10.1016/j.crmeth.2021.100137

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