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Enrichment of gut microbiome strains for cultivation-free genome sequencing using droplet microfluidics
We report a droplet microfluidic method to target and sort individual cells directly from complex microbiome samples and to prepare these cells for bulk whole-genome sequencing without cultivation. We characterize this approach by recovering bacteria spiked into human stool samples at a ratio as low...
| Autores principales: | , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8787643/ https://www.ncbi.nlm.nih.gov/pubmed/35118437 http://dx.doi.org/10.1016/j.crmeth.2021.100137 |