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Simplified Gene Knockout by CRISPR-Cas9-Induced Homologous Recombination

[Image: see text] Genetic engineering of industrial cell lines often requires knockout of multiple endogenous genes. Tools like CRISPR-Cas9 have enabled serial or parallelized gene disruption in a wide range of industrial organisms, but common practices for the screening and validation of genome edi...

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Detalles Bibliográficos
Autores principales:	Dalvie, Neil C., Lorgeree, Timothy, Biedermann, Andrew M., Love, Kerry R., Love, J. Christopher
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	American Chemical Society 2021
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8787811/ https://www.ncbi.nlm.nih.gov/pubmed/34882409 http://dx.doi.org/10.1021/acssynbio.1c00194

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