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pH-Triggered Assembly of Endomembrane Multicompartments in Synthetic Cells
[Image: see text] By using electrostatic interactions as driving force to assemble vesicles, the droplet-stabilized method was recently applied to reconstitute and encapsulate proteins, or compartments, inside giant unilamellar vesicles (GUVs) to act as minimal synthetic cells. However, the droplet-...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2021
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8787813/ https://www.ncbi.nlm.nih.gov/pubmed/34889607 http://dx.doi.org/10.1021/acssynbio.1c00472 |
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author | Lussier, Félix Schröter, Martin Diercks, Nicolas J. Jahnke, Kevin Weber, Cornelia Frey, Christoph Platzman, Ilia Spatz, Joachim P. |
author_facet | Lussier, Félix Schröter, Martin Diercks, Nicolas J. Jahnke, Kevin Weber, Cornelia Frey, Christoph Platzman, Ilia Spatz, Joachim P. |
author_sort | Lussier, Félix |
collection | PubMed |
description | [Image: see text] By using electrostatic interactions as driving force to assemble vesicles, the droplet-stabilized method was recently applied to reconstitute and encapsulate proteins, or compartments, inside giant unilamellar vesicles (GUVs) to act as minimal synthetic cells. However, the droplet-stabilized approach exhibits low production efficiency associated with the troublesome release of the GUVs from the stabilized droplets, corresponding to a major hurdle for the droplet-stabilized approach. Herein, we report the use of pH as a potential trigger to self-assemble droplet-stabilized GUVs (dsGUVs) by either bulk or droplet-based microfluidics. Moreover, pH enables the generation of compartmentalized GUVs with flexibility and robustness. By co-encapsulating pH-sensitive small unilamellar vesicles (SUVs), negatively charged SUVs, and/or proteins, we show that acidification of the droplets efficiently produces dsGUVs while sequestrating the co-encapsulated material. Most importantly, the pH-mediated assembly of dsGUVs significantly improves the production efficiency of free-standing GUVs (i.e., released from the stabilizing-droplets) compared to its previous implementation. |
format | Online Article Text |
id | pubmed-8787813 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-87878132022-01-26 pH-Triggered Assembly of Endomembrane Multicompartments in Synthetic Cells Lussier, Félix Schröter, Martin Diercks, Nicolas J. Jahnke, Kevin Weber, Cornelia Frey, Christoph Platzman, Ilia Spatz, Joachim P. ACS Synth Biol [Image: see text] By using electrostatic interactions as driving force to assemble vesicles, the droplet-stabilized method was recently applied to reconstitute and encapsulate proteins, or compartments, inside giant unilamellar vesicles (GUVs) to act as minimal synthetic cells. However, the droplet-stabilized approach exhibits low production efficiency associated with the troublesome release of the GUVs from the stabilized droplets, corresponding to a major hurdle for the droplet-stabilized approach. Herein, we report the use of pH as a potential trigger to self-assemble droplet-stabilized GUVs (dsGUVs) by either bulk or droplet-based microfluidics. Moreover, pH enables the generation of compartmentalized GUVs with flexibility and robustness. By co-encapsulating pH-sensitive small unilamellar vesicles (SUVs), negatively charged SUVs, and/or proteins, we show that acidification of the droplets efficiently produces dsGUVs while sequestrating the co-encapsulated material. Most importantly, the pH-mediated assembly of dsGUVs significantly improves the production efficiency of free-standing GUVs (i.e., released from the stabilizing-droplets) compared to its previous implementation. American Chemical Society 2021-12-10 2022-01-21 /pmc/articles/PMC8787813/ /pubmed/34889607 http://dx.doi.org/10.1021/acssynbio.1c00472 Text en © 2021 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Lussier, Félix Schröter, Martin Diercks, Nicolas J. Jahnke, Kevin Weber, Cornelia Frey, Christoph Platzman, Ilia Spatz, Joachim P. pH-Triggered Assembly of Endomembrane Multicompartments in Synthetic Cells |
title | pH-Triggered Assembly of Endomembrane Multicompartments
in Synthetic Cells |
title_full | pH-Triggered Assembly of Endomembrane Multicompartments
in Synthetic Cells |
title_fullStr | pH-Triggered Assembly of Endomembrane Multicompartments
in Synthetic Cells |
title_full_unstemmed | pH-Triggered Assembly of Endomembrane Multicompartments
in Synthetic Cells |
title_short | pH-Triggered Assembly of Endomembrane Multicompartments
in Synthetic Cells |
title_sort | ph-triggered assembly of endomembrane multicompartments
in synthetic cells |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8787813/ https://www.ncbi.nlm.nih.gov/pubmed/34889607 http://dx.doi.org/10.1021/acssynbio.1c00472 |
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