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Structural Probing with MNase Tethered to Ribosome Assembly Factors Resolves Flexible RNA Regions within the Nascent Pre-Ribosomal RNA

The synthesis of ribosomes involves the correct folding of the pre-ribosomal RNA within pre-ribosomal particles. The first ribosomal precursor or small subunit processome assembles stepwise on the nascent transcript of the 35S gene. At the earlier stages, the pre-ribosomal particles undergo structur...

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Autores principales: Dielforder, Tom, Braun, Christina Maria, Hölzgen, Fabian, Li, Shuang, Thiele, Mona, Huber, Marina, Ohmayer, Uli, Perez-Fernandez, Jorge
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8788456/
https://www.ncbi.nlm.nih.gov/pubmed/35076539
http://dx.doi.org/10.3390/ncrna8010001
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author Dielforder, Tom
Braun, Christina Maria
Hölzgen, Fabian
Li, Shuang
Thiele, Mona
Huber, Marina
Ohmayer, Uli
Perez-Fernandez, Jorge
author_facet Dielforder, Tom
Braun, Christina Maria
Hölzgen, Fabian
Li, Shuang
Thiele, Mona
Huber, Marina
Ohmayer, Uli
Perez-Fernandez, Jorge
author_sort Dielforder, Tom
collection PubMed
description The synthesis of ribosomes involves the correct folding of the pre-ribosomal RNA within pre-ribosomal particles. The first ribosomal precursor or small subunit processome assembles stepwise on the nascent transcript of the 35S gene. At the earlier stages, the pre-ribosomal particles undergo structural and compositional changes, resulting in heterogeneous populations of particles with highly flexible regions. Structural probing methods are suitable for resolving these structures and providing evidence about the architecture of ribonucleoprotein complexes. Our approach used MNase tethered to the assembly factors Nan1/Utp17, Utp10, Utp12, and Utp13, which among other factors, initiate the formation of the small subunit processome. Our results provide dynamic information about the folding of the pre-ribosomes by elucidating the relative organization of the 5′ETS and ITS1 regions within the 35S and U3 snoRNA around the C-terminal domains of Nan1/Utp17, Utp10, Utp12, and Utp13.
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spelling pubmed-87884562022-01-26 Structural Probing with MNase Tethered to Ribosome Assembly Factors Resolves Flexible RNA Regions within the Nascent Pre-Ribosomal RNA Dielforder, Tom Braun, Christina Maria Hölzgen, Fabian Li, Shuang Thiele, Mona Huber, Marina Ohmayer, Uli Perez-Fernandez, Jorge Noncoding RNA Article The synthesis of ribosomes involves the correct folding of the pre-ribosomal RNA within pre-ribosomal particles. The first ribosomal precursor or small subunit processome assembles stepwise on the nascent transcript of the 35S gene. At the earlier stages, the pre-ribosomal particles undergo structural and compositional changes, resulting in heterogeneous populations of particles with highly flexible regions. Structural probing methods are suitable for resolving these structures and providing evidence about the architecture of ribonucleoprotein complexes. Our approach used MNase tethered to the assembly factors Nan1/Utp17, Utp10, Utp12, and Utp13, which among other factors, initiate the formation of the small subunit processome. Our results provide dynamic information about the folding of the pre-ribosomes by elucidating the relative organization of the 5′ETS and ITS1 regions within the 35S and U3 snoRNA around the C-terminal domains of Nan1/Utp17, Utp10, Utp12, and Utp13. MDPI 2022-01-09 /pmc/articles/PMC8788456/ /pubmed/35076539 http://dx.doi.org/10.3390/ncrna8010001 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Dielforder, Tom
Braun, Christina Maria
Hölzgen, Fabian
Li, Shuang
Thiele, Mona
Huber, Marina
Ohmayer, Uli
Perez-Fernandez, Jorge
Structural Probing with MNase Tethered to Ribosome Assembly Factors Resolves Flexible RNA Regions within the Nascent Pre-Ribosomal RNA
title Structural Probing with MNase Tethered to Ribosome Assembly Factors Resolves Flexible RNA Regions within the Nascent Pre-Ribosomal RNA
title_full Structural Probing with MNase Tethered to Ribosome Assembly Factors Resolves Flexible RNA Regions within the Nascent Pre-Ribosomal RNA
title_fullStr Structural Probing with MNase Tethered to Ribosome Assembly Factors Resolves Flexible RNA Regions within the Nascent Pre-Ribosomal RNA
title_full_unstemmed Structural Probing with MNase Tethered to Ribosome Assembly Factors Resolves Flexible RNA Regions within the Nascent Pre-Ribosomal RNA
title_short Structural Probing with MNase Tethered to Ribosome Assembly Factors Resolves Flexible RNA Regions within the Nascent Pre-Ribosomal RNA
title_sort structural probing with mnase tethered to ribosome assembly factors resolves flexible rna regions within the nascent pre-ribosomal rna
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8788456/
https://www.ncbi.nlm.nih.gov/pubmed/35076539
http://dx.doi.org/10.3390/ncrna8010001
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