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PCA-Assisted Raman Analysis of Osteonecrotic Human Femoral Heads

Osteonecrosis of the femoral head (ONFH) occurs frequently in adolescents and young adults and causes progressive deformation and destruction of the hip joint and impairs standing and walking, resulting in a significant decrease in the quality of life of patients. In addition, studies have shown tha...

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Autores principales: Ishimura, Eiji, Zhu, Wenliang, Marin, Elia, Honma, Taigi, Sugano, Nobuhiko, Ando, Wataru, Pezzotti, Giuseppe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8788499/
https://www.ncbi.nlm.nih.gov/pubmed/35076564
http://dx.doi.org/10.3390/mps5010010
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author Ishimura, Eiji
Zhu, Wenliang
Marin, Elia
Honma, Taigi
Sugano, Nobuhiko
Ando, Wataru
Pezzotti, Giuseppe
author_facet Ishimura, Eiji
Zhu, Wenliang
Marin, Elia
Honma, Taigi
Sugano, Nobuhiko
Ando, Wataru
Pezzotti, Giuseppe
author_sort Ishimura, Eiji
collection PubMed
description Osteonecrosis of the femoral head (ONFH) occurs frequently in adolescents and young adults and causes progressive deformation and destruction of the hip joint and impairs standing and walking, resulting in a significant decrease in the quality of life of patients. In addition, studies have shown that a history of corticosteroid administration and heavy alcohol consumption are closely related to the occurrence of ONFH. However, the detailed mechanism by which steroid administration and alcohol consumption are associated with the development of the disease is still unknown. With many researches still ongoing and without a clear biological pathway for osteonecrosis, effective preventive measures cannot be taken. Therefore, the current focus of ONFH treatment is to establish an early diagnosis and treatment strategy. We obtained the femoral heads of four patients with steroidal ONFH and three patients with alcoholic ONFH. We then compared the femoral heads of steroidal and alcoholic osteonecrosis by analyzing them at the molecular level by Raman spectroscopy. Crystallographic changes (deformations) in the mineral phase and fraction of organic material respect to the total mass were then plotted as a function. We found that changes in bone composition in ONFH were different in steroidal and alcoholic ONFH. We conclude that this suggests that the developmental mechanisms of steroidal and alcoholic ONFH may follow different paths. We also noticed that while steroid seem to lead to a more marked degradation of the tissue, alcohol seem to affect also the quality of the healthy tissue.
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spelling pubmed-87884992022-01-26 PCA-Assisted Raman Analysis of Osteonecrotic Human Femoral Heads Ishimura, Eiji Zhu, Wenliang Marin, Elia Honma, Taigi Sugano, Nobuhiko Ando, Wataru Pezzotti, Giuseppe Methods Protoc Article Osteonecrosis of the femoral head (ONFH) occurs frequently in adolescents and young adults and causes progressive deformation and destruction of the hip joint and impairs standing and walking, resulting in a significant decrease in the quality of life of patients. In addition, studies have shown that a history of corticosteroid administration and heavy alcohol consumption are closely related to the occurrence of ONFH. However, the detailed mechanism by which steroid administration and alcohol consumption are associated with the development of the disease is still unknown. With many researches still ongoing and without a clear biological pathway for osteonecrosis, effective preventive measures cannot be taken. Therefore, the current focus of ONFH treatment is to establish an early diagnosis and treatment strategy. We obtained the femoral heads of four patients with steroidal ONFH and three patients with alcoholic ONFH. We then compared the femoral heads of steroidal and alcoholic osteonecrosis by analyzing them at the molecular level by Raman spectroscopy. Crystallographic changes (deformations) in the mineral phase and fraction of organic material respect to the total mass were then plotted as a function. We found that changes in bone composition in ONFH were different in steroidal and alcoholic ONFH. We conclude that this suggests that the developmental mechanisms of steroidal and alcoholic ONFH may follow different paths. We also noticed that while steroid seem to lead to a more marked degradation of the tissue, alcohol seem to affect also the quality of the healthy tissue. MDPI 2022-01-17 /pmc/articles/PMC8788499/ /pubmed/35076564 http://dx.doi.org/10.3390/mps5010010 Text en © 2022 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ishimura, Eiji
Zhu, Wenliang
Marin, Elia
Honma, Taigi
Sugano, Nobuhiko
Ando, Wataru
Pezzotti, Giuseppe
PCA-Assisted Raman Analysis of Osteonecrotic Human Femoral Heads
title PCA-Assisted Raman Analysis of Osteonecrotic Human Femoral Heads
title_full PCA-Assisted Raman Analysis of Osteonecrotic Human Femoral Heads
title_fullStr PCA-Assisted Raman Analysis of Osteonecrotic Human Femoral Heads
title_full_unstemmed PCA-Assisted Raman Analysis of Osteonecrotic Human Femoral Heads
title_short PCA-Assisted Raman Analysis of Osteonecrotic Human Femoral Heads
title_sort pca-assisted raman analysis of osteonecrotic human femoral heads
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8788499/
https://www.ncbi.nlm.nih.gov/pubmed/35076564
http://dx.doi.org/10.3390/mps5010010
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