Cargando…
RhoA, Claudin 18, and c-MET in Gastric Cancer: Clinicopathological Characteristics and Prognostic Significance in Curative Resected Patients
Background: Recently, markers related to molecular classification were suggested as promising therapeutic targets for treatment and prediction of prognosis in gastric cancer (GC), including c-MET, RhoA, and Claudin-18 (CLDN18). This study aimed to investigate their expression in GC and its correlati...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8788521/ https://www.ncbi.nlm.nih.gov/pubmed/35076580 http://dx.doi.org/10.3390/medsci10010004 |
_version_ | 1784639584778321920 |
---|---|
author | Pereira, Marina Alessandra Ramos, Marcus Fernando Kodama Pertille Dias, Andre Roncon Cardili, Leonardo Ribeiro, Renan Ribeiro e de Castria, Tiago Biachi Zilberstein, Bruno Nahas, Sergio Carlos Ribeiro, Ulysses de Mello, Evandro Sobroza |
author_facet | Pereira, Marina Alessandra Ramos, Marcus Fernando Kodama Pertille Dias, Andre Roncon Cardili, Leonardo Ribeiro, Renan Ribeiro e de Castria, Tiago Biachi Zilberstein, Bruno Nahas, Sergio Carlos Ribeiro, Ulysses de Mello, Evandro Sobroza |
author_sort | Pereira, Marina Alessandra |
collection | PubMed |
description | Background: Recently, markers related to molecular classification were suggested as promising therapeutic targets for treatment and prediction of prognosis in gastric cancer (GC), including c-MET, RhoA, and Claudin-18 (CLDN18). This study aimed to investigate their expression in GC and its correlation with clinicopathological characteristics and survival. Methods: We retrospectively evaluated GC patients who underwent curative gastrectomy. c-MET, RhoA, and CLDN18 were analyzed through immunohistochemistry (IHC), and groups for analysis were determined according to the median values obtained for each marker. Results: Among the 349 GC evaluated, 180 (51.6%), 59 (16.9%), and 61 (17.5%) patients were completely negative for c-MET, RhoA, and CLDN18, respectively. Total gastrectomy, D1 lymphadenectomy, poorly differentiated histology, and greater inflammatory infiltrate were more frequent in the c-MET-negative group. Diffuse type, greater inflammatory infiltrate, and advanced pT and pTNM stage were associated with low-RhoA GC. The venous invasion was more frequent in the low-CLDN18 group. Furthermore, c-MET was positively correlated with RhoA and negatively with CLDN18. HER2 expression was associated with c-MET-positive and high-CLDN18 GC; and loss of E-cadherin expression in c-MET-negative and low-RhoA GC. c-MET-negative and Low-RhoA were significantly associated with worse disease-free survival. Conclusions: c-MET, RhoA, and CLD18 expression occurred frequently in GC. RhoA GC had distinct clinicopathological characteristics related to prognosis. c-MET and RhoA were associated with survival but were not independent predictors of prognosis. |
format | Online Article Text |
id | pubmed-8788521 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-87885212022-01-26 RhoA, Claudin 18, and c-MET in Gastric Cancer: Clinicopathological Characteristics and Prognostic Significance in Curative Resected Patients Pereira, Marina Alessandra Ramos, Marcus Fernando Kodama Pertille Dias, Andre Roncon Cardili, Leonardo Ribeiro, Renan Ribeiro e de Castria, Tiago Biachi Zilberstein, Bruno Nahas, Sergio Carlos Ribeiro, Ulysses de Mello, Evandro Sobroza Med Sci (Basel) Article Background: Recently, markers related to molecular classification were suggested as promising therapeutic targets for treatment and prediction of prognosis in gastric cancer (GC), including c-MET, RhoA, and Claudin-18 (CLDN18). This study aimed to investigate their expression in GC and its correlation with clinicopathological characteristics and survival. Methods: We retrospectively evaluated GC patients who underwent curative gastrectomy. c-MET, RhoA, and CLDN18 were analyzed through immunohistochemistry (IHC), and groups for analysis were determined according to the median values obtained for each marker. Results: Among the 349 GC evaluated, 180 (51.6%), 59 (16.9%), and 61 (17.5%) patients were completely negative for c-MET, RhoA, and CLDN18, respectively. Total gastrectomy, D1 lymphadenectomy, poorly differentiated histology, and greater inflammatory infiltrate were more frequent in the c-MET-negative group. Diffuse type, greater inflammatory infiltrate, and advanced pT and pTNM stage were associated with low-RhoA GC. The venous invasion was more frequent in the low-CLDN18 group. Furthermore, c-MET was positively correlated with RhoA and negatively with CLDN18. HER2 expression was associated with c-MET-positive and high-CLDN18 GC; and loss of E-cadherin expression in c-MET-negative and low-RhoA GC. c-MET-negative and Low-RhoA were significantly associated with worse disease-free survival. Conclusions: c-MET, RhoA, and CLD18 expression occurred frequently in GC. RhoA GC had distinct clinicopathological characteristics related to prognosis. c-MET and RhoA were associated with survival but were not independent predictors of prognosis. MDPI 2021-12-29 /pmc/articles/PMC8788521/ /pubmed/35076580 http://dx.doi.org/10.3390/medsci10010004 Text en © 2021 by the authors. https://creativecommons.org/licenses/by/4.0/Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Pereira, Marina Alessandra Ramos, Marcus Fernando Kodama Pertille Dias, Andre Roncon Cardili, Leonardo Ribeiro, Renan Ribeiro e de Castria, Tiago Biachi Zilberstein, Bruno Nahas, Sergio Carlos Ribeiro, Ulysses de Mello, Evandro Sobroza RhoA, Claudin 18, and c-MET in Gastric Cancer: Clinicopathological Characteristics and Prognostic Significance in Curative Resected Patients |
title | RhoA, Claudin 18, and c-MET in Gastric Cancer: Clinicopathological Characteristics and Prognostic Significance in Curative Resected Patients |
title_full | RhoA, Claudin 18, and c-MET in Gastric Cancer: Clinicopathological Characteristics and Prognostic Significance in Curative Resected Patients |
title_fullStr | RhoA, Claudin 18, and c-MET in Gastric Cancer: Clinicopathological Characteristics and Prognostic Significance in Curative Resected Patients |
title_full_unstemmed | RhoA, Claudin 18, and c-MET in Gastric Cancer: Clinicopathological Characteristics and Prognostic Significance in Curative Resected Patients |
title_short | RhoA, Claudin 18, and c-MET in Gastric Cancer: Clinicopathological Characteristics and Prognostic Significance in Curative Resected Patients |
title_sort | rhoa, claudin 18, and c-met in gastric cancer: clinicopathological characteristics and prognostic significance in curative resected patients |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8788521/ https://www.ncbi.nlm.nih.gov/pubmed/35076580 http://dx.doi.org/10.3390/medsci10010004 |
work_keys_str_mv | AT pereiramarinaalessandra rhoaclaudin18andcmetingastriccancerclinicopathologicalcharacteristicsandprognosticsignificanceincurativeresectedpatients AT ramosmarcusfernandokodamapertille rhoaclaudin18andcmetingastriccancerclinicopathologicalcharacteristicsandprognosticsignificanceincurativeresectedpatients AT diasandreroncon rhoaclaudin18andcmetingastriccancerclinicopathologicalcharacteristicsandprognosticsignificanceincurativeresectedpatients AT cardilileonardo rhoaclaudin18andcmetingastriccancerclinicopathologicalcharacteristicsandprognosticsignificanceincurativeresectedpatients AT ribeirorenanribeiroe rhoaclaudin18andcmetingastriccancerclinicopathologicalcharacteristicsandprognosticsignificanceincurativeresectedpatients AT decastriatiagobiachi rhoaclaudin18andcmetingastriccancerclinicopathologicalcharacteristicsandprognosticsignificanceincurativeresectedpatients AT zilbersteinbruno rhoaclaudin18andcmetingastriccancerclinicopathologicalcharacteristicsandprognosticsignificanceincurativeresectedpatients AT nahassergiocarlos rhoaclaudin18andcmetingastriccancerclinicopathologicalcharacteristicsandprognosticsignificanceincurativeresectedpatients AT ribeiroulysses rhoaclaudin18andcmetingastriccancerclinicopathologicalcharacteristicsandprognosticsignificanceincurativeresectedpatients AT demelloevandrosobroza rhoaclaudin18andcmetingastriccancerclinicopathologicalcharacteristicsandprognosticsignificanceincurativeresectedpatients |