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Random sperm DNA fragmentation index is not associated with clinical outcomes in day-3 frozen embryo transfer

Damage to sperm DNA was proposed to play an important role in embryonic development. Previous studies focused on outcomes after fresh embryo transfer, whereas this study investigated the influence of sperm DNA fragmentation index (DFI) on laboratory and clinical outcomes after frozen embryo transfer...

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Autores principales: Wang, Qing-Xin, Wang, Xia, Yu, Min-Yan, Sun, Hua, Wang, Di, Zhong, Shu-Ping, Guo, Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wolters Kluwer - Medknow 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8788616/
https://www.ncbi.nlm.nih.gov/pubmed/33835076
http://dx.doi.org/10.4103/aja.aja_17_21
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author Wang, Qing-Xin
Wang, Xia
Yu, Min-Yan
Sun, Hua
Wang, Di
Zhong, Shu-Ping
Guo, Feng
author_facet Wang, Qing-Xin
Wang, Xia
Yu, Min-Yan
Sun, Hua
Wang, Di
Zhong, Shu-Ping
Guo, Feng
author_sort Wang, Qing-Xin
collection PubMed
description Damage to sperm DNA was proposed to play an important role in embryonic development. Previous studies focused on outcomes after fresh embryo transfer, whereas this study investigated the influence of sperm DNA fragmentation index (DFI) on laboratory and clinical outcomes after frozen embryo transfer (FET). This retrospective study examined 381 couples using cleavage-stage FET. Sperm used for intracytoplasmic sperm injection (ICSI) or in vitro fertilization (IVF) underwent density gradient centrifugation and swim up processing. Sperm DFI had a negative correlation with sperm motility (r = −0.640, P < 0.01), sperm concentration (r = −0.289, P < 0.01), and fertilization rate of IVF cycles (r = −0.247, P < 0.01). Sperm DFI examined before and after density gradient centrifugation/swim up processing was markedly decreased after processing (17.1% vs 2.4%, P < 0.01; 65 randomly picked couples). Sperm progressive motility was significantly reduced in high DFI group compared with low DFI group for both IVF and ICSI (IVF: 46.9% ± 12.4% vs 38.5% ± 12.6%, respectively; ICSI: 37.6% ± 14.1% vs 22.3% ± 17.8%, respectively; both P < 0.01). The fertilization rate was significantly lower in high (≥25%) DFI group compared with low (<25%) DFI group using IVF (73.3% ± 23.9% vs 53.2% ± 33.6%, respectively; P < 0.01) but was equivalent in high and low DFI groups using ICSI. Embryonic development and clinical outcomes after FET were equivalent for low and high DFI groups using ICSI or IVF. In this study, sperm DFI did not provide sufficient information regarding embryo development or clinical outcomes for infertile couples using FET.
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spelling pubmed-87886162022-02-03 Random sperm DNA fragmentation index is not associated with clinical outcomes in day-3 frozen embryo transfer Wang, Qing-Xin Wang, Xia Yu, Min-Yan Sun, Hua Wang, Di Zhong, Shu-Ping Guo, Feng Asian J Androl Original Article Damage to sperm DNA was proposed to play an important role in embryonic development. Previous studies focused on outcomes after fresh embryo transfer, whereas this study investigated the influence of sperm DNA fragmentation index (DFI) on laboratory and clinical outcomes after frozen embryo transfer (FET). This retrospective study examined 381 couples using cleavage-stage FET. Sperm used for intracytoplasmic sperm injection (ICSI) or in vitro fertilization (IVF) underwent density gradient centrifugation and swim up processing. Sperm DFI had a negative correlation with sperm motility (r = −0.640, P < 0.01), sperm concentration (r = −0.289, P < 0.01), and fertilization rate of IVF cycles (r = −0.247, P < 0.01). Sperm DFI examined before and after density gradient centrifugation/swim up processing was markedly decreased after processing (17.1% vs 2.4%, P < 0.01; 65 randomly picked couples). Sperm progressive motility was significantly reduced in high DFI group compared with low DFI group for both IVF and ICSI (IVF: 46.9% ± 12.4% vs 38.5% ± 12.6%, respectively; ICSI: 37.6% ± 14.1% vs 22.3% ± 17.8%, respectively; both P < 0.01). The fertilization rate was significantly lower in high (≥25%) DFI group compared with low (<25%) DFI group using IVF (73.3% ± 23.9% vs 53.2% ± 33.6%, respectively; P < 0.01) but was equivalent in high and low DFI groups using ICSI. Embryonic development and clinical outcomes after FET were equivalent for low and high DFI groups using ICSI or IVF. In this study, sperm DFI did not provide sufficient information regarding embryo development or clinical outcomes for infertile couples using FET. Wolters Kluwer - Medknow 2021-04-06 /pmc/articles/PMC8788616/ /pubmed/33835076 http://dx.doi.org/10.4103/aja.aja_17_21 Text en Copyright: ©The Author(s)(2021) https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms.
spellingShingle Original Article
Wang, Qing-Xin
Wang, Xia
Yu, Min-Yan
Sun, Hua
Wang, Di
Zhong, Shu-Ping
Guo, Feng
Random sperm DNA fragmentation index is not associated with clinical outcomes in day-3 frozen embryo transfer
title Random sperm DNA fragmentation index is not associated with clinical outcomes in day-3 frozen embryo transfer
title_full Random sperm DNA fragmentation index is not associated with clinical outcomes in day-3 frozen embryo transfer
title_fullStr Random sperm DNA fragmentation index is not associated with clinical outcomes in day-3 frozen embryo transfer
title_full_unstemmed Random sperm DNA fragmentation index is not associated with clinical outcomes in day-3 frozen embryo transfer
title_short Random sperm DNA fragmentation index is not associated with clinical outcomes in day-3 frozen embryo transfer
title_sort random sperm dna fragmentation index is not associated with clinical outcomes in day-3 frozen embryo transfer
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8788616/
https://www.ncbi.nlm.nih.gov/pubmed/33835076
http://dx.doi.org/10.4103/aja.aja_17_21
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