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Characterization of cross-species transcription and splicing from Penicillium to Saccharomyces cerevisiae

Heterologous expression of eukaryotic gene clusters in yeast has been widely used for producing high-value chemicals and bioactive secondary metabolites. However, eukaryotic transcription cis-elements are still undercharacterized, and the cross-species expression mechanism remains poorly understood....

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Autores principales: Lin, Zhenquan, Xu, Kang, Cai, Guang, Liu, Yangqingxue, Li, Yi, Zhang, Zhihao, Nielsen, Jens, Shi, Shuobo, Liu, Zihe
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8788760/
https://www.ncbi.nlm.nih.gov/pubmed/34387324
http://dx.doi.org/10.1093/jimb/kuab054
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author Lin, Zhenquan
Xu, Kang
Cai, Guang
Liu, Yangqingxue
Li, Yi
Zhang, Zhihao
Nielsen, Jens
Shi, Shuobo
Liu, Zihe
author_facet Lin, Zhenquan
Xu, Kang
Cai, Guang
Liu, Yangqingxue
Li, Yi
Zhang, Zhihao
Nielsen, Jens
Shi, Shuobo
Liu, Zihe
author_sort Lin, Zhenquan
collection PubMed
description Heterologous expression of eukaryotic gene clusters in yeast has been widely used for producing high-value chemicals and bioactive secondary metabolites. However, eukaryotic transcription cis-elements are still undercharacterized, and the cross-species expression mechanism remains poorly understood. Here we used the whole expression unit (including original promoter, terminator, and open reading frame with introns) of orotidine 5′-monophosphate decarboxylases from 14 Penicillium species as a showcase, and analyzed their cross-species expression in Saccharomyces cerevisiae. We found that pyrG promoters from the Penicillium species could drive URA3 expression in yeast, and that inefficient cross-species splicing of Penicillium introns might result in weak cross-species expression. Thus, this study demonstrates cross-species expression from Penicillium to yeast, and sheds light on the opportunities and challenges of cross-species expression of fungi expression units and gene clusters in yeast without refactoring for novel natural product discovery.
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spelling pubmed-87887602022-06-08 Characterization of cross-species transcription and splicing from Penicillium to Saccharomyces cerevisiae Lin, Zhenquan Xu, Kang Cai, Guang Liu, Yangqingxue Li, Yi Zhang, Zhihao Nielsen, Jens Shi, Shuobo Liu, Zihe J Ind Microbiol Biotechnol Natural Products Heterologous expression of eukaryotic gene clusters in yeast has been widely used for producing high-value chemicals and bioactive secondary metabolites. However, eukaryotic transcription cis-elements are still undercharacterized, and the cross-species expression mechanism remains poorly understood. Here we used the whole expression unit (including original promoter, terminator, and open reading frame with introns) of orotidine 5′-monophosphate decarboxylases from 14 Penicillium species as a showcase, and analyzed their cross-species expression in Saccharomyces cerevisiae. We found that pyrG promoters from the Penicillium species could drive URA3 expression in yeast, and that inefficient cross-species splicing of Penicillium introns might result in weak cross-species expression. Thus, this study demonstrates cross-species expression from Penicillium to yeast, and sheds light on the opportunities and challenges of cross-species expression of fungi expression units and gene clusters in yeast without refactoring for novel natural product discovery. Oxford University Press 2021-08-13 /pmc/articles/PMC8788760/ /pubmed/34387324 http://dx.doi.org/10.1093/jimb/kuab054 Text en © The Author(s) 2021. Published by Oxford University Press on behalf of Society of Industrial Microbiology and Biotechnology. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Natural Products
Lin, Zhenquan
Xu, Kang
Cai, Guang
Liu, Yangqingxue
Li, Yi
Zhang, Zhihao
Nielsen, Jens
Shi, Shuobo
Liu, Zihe
Characterization of cross-species transcription and splicing from Penicillium to Saccharomyces cerevisiae
title Characterization of cross-species transcription and splicing from Penicillium to Saccharomyces cerevisiae
title_full Characterization of cross-species transcription and splicing from Penicillium to Saccharomyces cerevisiae
title_fullStr Characterization of cross-species transcription and splicing from Penicillium to Saccharomyces cerevisiae
title_full_unstemmed Characterization of cross-species transcription and splicing from Penicillium to Saccharomyces cerevisiae
title_short Characterization of cross-species transcription and splicing from Penicillium to Saccharomyces cerevisiae
title_sort characterization of cross-species transcription and splicing from penicillium to saccharomyces cerevisiae
topic Natural Products
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8788760/
https://www.ncbi.nlm.nih.gov/pubmed/34387324
http://dx.doi.org/10.1093/jimb/kuab054
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