Uncertainty-aware and interpretable evaluation of Cas9–gRNA and Cas12a–gRNA specificity for fully matched and partially mismatched targets with Deep Kernel Learning

The choice of guide RNA (gRNA) for CRISPR-based gene targeting is an essential step in gene editing applications, but the prediction of gRNA specificity remains challenging. Lack of transparency and focus on point estimates of efficiency disregarding the information on possible error sources in the...

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Detalles Bibliográficos
Autores principales: Kirillov, Bogdan, Savitskaya, Ekaterina, Panov, Maxim, Ogurtsov, Aleksey Y, Shabalina, Svetlana A, Koonin, Eugene V, Severinov, Konstantin V
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2021
Materias:
Methods Online
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8789050/
https://www.ncbi.nlm.nih.gov/pubmed/34791389
http://dx.doi.org/10.1093/nar/gkab1065

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