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Single-cell immune profiling reveals functional diversity of T cells in tuberculous pleural effusion
Orchestration of an effective T lymphocyte response at infection sites is critical for protection against Mycobacterium tuberculosis (Mtb) infection. However, the local T cell immunity landscape in human tuberculosis is poorly defined. Tuberculous pleural effusion (TPE), caused by Mtb, is characteri...
Autores principales: | , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Rockefeller University Press
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8789099/ https://www.ncbi.nlm.nih.gov/pubmed/35061012 http://dx.doi.org/10.1084/jem.20211777 |
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author | Cai, Yi Wang, Yejun Shi, Chenyan Dai, Youchao Li, Fuxiang Xu, Yuzhong Zhang, Peize Kong, Fanhui Deng, Guofang Wen, Zhihua Zhou, Qi Kang, Boxi Chris Singhal, Amit Yang, Qianting Feng, Carl G. Chen, Xinchun |
author_facet | Cai, Yi Wang, Yejun Shi, Chenyan Dai, Youchao Li, Fuxiang Xu, Yuzhong Zhang, Peize Kong, Fanhui Deng, Guofang Wen, Zhihua Zhou, Qi Kang, Boxi Chris Singhal, Amit Yang, Qianting Feng, Carl G. Chen, Xinchun |
author_sort | Cai, Yi |
collection | PubMed |
description | Orchestration of an effective T lymphocyte response at infection sites is critical for protection against Mycobacterium tuberculosis (Mtb) infection. However, the local T cell immunity landscape in human tuberculosis is poorly defined. Tuberculous pleural effusion (TPE), caused by Mtb, is characterized by an influx of leukocytes to the pleural space, providing a platform suitable for delineating complex tissue responses to Mtb infection. Using single-cell transcriptomics and T cell receptor sequencing, we analyzed mononuclear cell populations in paired pleural fluid and peripheral blood of TPE patients. While all major cell clusters were present in both tissues, their relative proportions varied significantly by anatomic location. Lineage tracking analysis revealed subsets of CD8 and CD4 T cell populations with distinct effector functions specifically expanded at pleural sites. Granzyme K–expressing CD8 T cells were preferentially enriched and clonally expanded in pleural fluid from TPE, suggesting that they are involved in the pathogenesis of the disease. The findings collectively reveal the landscape of local T cell immunity in tuberculosis. |
format | Online Article Text |
id | pubmed-8789099 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Rockefeller University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-87890992022-09-07 Single-cell immune profiling reveals functional diversity of T cells in tuberculous pleural effusion Cai, Yi Wang, Yejun Shi, Chenyan Dai, Youchao Li, Fuxiang Xu, Yuzhong Zhang, Peize Kong, Fanhui Deng, Guofang Wen, Zhihua Zhou, Qi Kang, Boxi Chris Singhal, Amit Yang, Qianting Feng, Carl G. Chen, Xinchun J Exp Med Technical Advances and Resources Orchestration of an effective T lymphocyte response at infection sites is critical for protection against Mycobacterium tuberculosis (Mtb) infection. However, the local T cell immunity landscape in human tuberculosis is poorly defined. Tuberculous pleural effusion (TPE), caused by Mtb, is characterized by an influx of leukocytes to the pleural space, providing a platform suitable for delineating complex tissue responses to Mtb infection. Using single-cell transcriptomics and T cell receptor sequencing, we analyzed mononuclear cell populations in paired pleural fluid and peripheral blood of TPE patients. While all major cell clusters were present in both tissues, their relative proportions varied significantly by anatomic location. Lineage tracking analysis revealed subsets of CD8 and CD4 T cell populations with distinct effector functions specifically expanded at pleural sites. Granzyme K–expressing CD8 T cells were preferentially enriched and clonally expanded in pleural fluid from TPE, suggesting that they are involved in the pathogenesis of the disease. The findings collectively reveal the landscape of local T cell immunity in tuberculosis. Rockefeller University Press 2022-01-21 /pmc/articles/PMC8789099/ /pubmed/35061012 http://dx.doi.org/10.1084/jem.20211777 Text en © 2022 Cai et al. https://creativecommons.org/licenses/by-nc-sa/4.0/http://www.rupress.org/terms/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/). |
spellingShingle | Technical Advances and Resources Cai, Yi Wang, Yejun Shi, Chenyan Dai, Youchao Li, Fuxiang Xu, Yuzhong Zhang, Peize Kong, Fanhui Deng, Guofang Wen, Zhihua Zhou, Qi Kang, Boxi Chris Singhal, Amit Yang, Qianting Feng, Carl G. Chen, Xinchun Single-cell immune profiling reveals functional diversity of T cells in tuberculous pleural effusion |
title | Single-cell immune profiling reveals functional diversity of T cells in tuberculous pleural effusion |
title_full | Single-cell immune profiling reveals functional diversity of T cells in tuberculous pleural effusion |
title_fullStr | Single-cell immune profiling reveals functional diversity of T cells in tuberculous pleural effusion |
title_full_unstemmed | Single-cell immune profiling reveals functional diversity of T cells in tuberculous pleural effusion |
title_short | Single-cell immune profiling reveals functional diversity of T cells in tuberculous pleural effusion |
title_sort | single-cell immune profiling reveals functional diversity of t cells in tuberculous pleural effusion |
topic | Technical Advances and Resources |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8789099/ https://www.ncbi.nlm.nih.gov/pubmed/35061012 http://dx.doi.org/10.1084/jem.20211777 |
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