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Single-cell immune profiling reveals functional diversity of T cells in tuberculous pleural effusion

Orchestration of an effective T lymphocyte response at infection sites is critical for protection against Mycobacterium tuberculosis (Mtb) infection. However, the local T cell immunity landscape in human tuberculosis is poorly defined. Tuberculous pleural effusion (TPE), caused by Mtb, is characteri...

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Autores principales: Cai, Yi, Wang, Yejun, Shi, Chenyan, Dai, Youchao, Li, Fuxiang, Xu, Yuzhong, Zhang, Peize, Kong, Fanhui, Deng, Guofang, Wen, Zhihua, Zhou, Qi, Kang, Boxi Chris, Singhal, Amit, Yang, Qianting, Feng, Carl G., Chen, Xinchun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Rockefeller University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8789099/
https://www.ncbi.nlm.nih.gov/pubmed/35061012
http://dx.doi.org/10.1084/jem.20211777
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author Cai, Yi
Wang, Yejun
Shi, Chenyan
Dai, Youchao
Li, Fuxiang
Xu, Yuzhong
Zhang, Peize
Kong, Fanhui
Deng, Guofang
Wen, Zhihua
Zhou, Qi
Kang, Boxi Chris
Singhal, Amit
Yang, Qianting
Feng, Carl G.
Chen, Xinchun
author_facet Cai, Yi
Wang, Yejun
Shi, Chenyan
Dai, Youchao
Li, Fuxiang
Xu, Yuzhong
Zhang, Peize
Kong, Fanhui
Deng, Guofang
Wen, Zhihua
Zhou, Qi
Kang, Boxi Chris
Singhal, Amit
Yang, Qianting
Feng, Carl G.
Chen, Xinchun
author_sort Cai, Yi
collection PubMed
description Orchestration of an effective T lymphocyte response at infection sites is critical for protection against Mycobacterium tuberculosis (Mtb) infection. However, the local T cell immunity landscape in human tuberculosis is poorly defined. Tuberculous pleural effusion (TPE), caused by Mtb, is characterized by an influx of leukocytes to the pleural space, providing a platform suitable for delineating complex tissue responses to Mtb infection. Using single-cell transcriptomics and T cell receptor sequencing, we analyzed mononuclear cell populations in paired pleural fluid and peripheral blood of TPE patients. While all major cell clusters were present in both tissues, their relative proportions varied significantly by anatomic location. Lineage tracking analysis revealed subsets of CD8 and CD4 T cell populations with distinct effector functions specifically expanded at pleural sites. Granzyme K–expressing CD8 T cells were preferentially enriched and clonally expanded in pleural fluid from TPE, suggesting that they are involved in the pathogenesis of the disease. The findings collectively reveal the landscape of local T cell immunity in tuberculosis.
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spelling pubmed-87890992022-09-07 Single-cell immune profiling reveals functional diversity of T cells in tuberculous pleural effusion Cai, Yi Wang, Yejun Shi, Chenyan Dai, Youchao Li, Fuxiang Xu, Yuzhong Zhang, Peize Kong, Fanhui Deng, Guofang Wen, Zhihua Zhou, Qi Kang, Boxi Chris Singhal, Amit Yang, Qianting Feng, Carl G. Chen, Xinchun J Exp Med Technical Advances and Resources Orchestration of an effective T lymphocyte response at infection sites is critical for protection against Mycobacterium tuberculosis (Mtb) infection. However, the local T cell immunity landscape in human tuberculosis is poorly defined. Tuberculous pleural effusion (TPE), caused by Mtb, is characterized by an influx of leukocytes to the pleural space, providing a platform suitable for delineating complex tissue responses to Mtb infection. Using single-cell transcriptomics and T cell receptor sequencing, we analyzed mononuclear cell populations in paired pleural fluid and peripheral blood of TPE patients. While all major cell clusters were present in both tissues, their relative proportions varied significantly by anatomic location. Lineage tracking analysis revealed subsets of CD8 and CD4 T cell populations with distinct effector functions specifically expanded at pleural sites. Granzyme K–expressing CD8 T cells were preferentially enriched and clonally expanded in pleural fluid from TPE, suggesting that they are involved in the pathogenesis of the disease. The findings collectively reveal the landscape of local T cell immunity in tuberculosis. Rockefeller University Press 2022-01-21 /pmc/articles/PMC8789099/ /pubmed/35061012 http://dx.doi.org/10.1084/jem.20211777 Text en © 2022 Cai et al. https://creativecommons.org/licenses/by-nc-sa/4.0/http://www.rupress.org/terms/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Technical Advances and Resources
Cai, Yi
Wang, Yejun
Shi, Chenyan
Dai, Youchao
Li, Fuxiang
Xu, Yuzhong
Zhang, Peize
Kong, Fanhui
Deng, Guofang
Wen, Zhihua
Zhou, Qi
Kang, Boxi Chris
Singhal, Amit
Yang, Qianting
Feng, Carl G.
Chen, Xinchun
Single-cell immune profiling reveals functional diversity of T cells in tuberculous pleural effusion
title Single-cell immune profiling reveals functional diversity of T cells in tuberculous pleural effusion
title_full Single-cell immune profiling reveals functional diversity of T cells in tuberculous pleural effusion
title_fullStr Single-cell immune profiling reveals functional diversity of T cells in tuberculous pleural effusion
title_full_unstemmed Single-cell immune profiling reveals functional diversity of T cells in tuberculous pleural effusion
title_short Single-cell immune profiling reveals functional diversity of T cells in tuberculous pleural effusion
title_sort single-cell immune profiling reveals functional diversity of t cells in tuberculous pleural effusion
topic Technical Advances and Resources
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8789099/
https://www.ncbi.nlm.nih.gov/pubmed/35061012
http://dx.doi.org/10.1084/jem.20211777
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