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In vitro fluorescence assay to measure GDP/GTP exchange of guanine nucleotide exchange factors of Rho family GTPases

Guanine nucleotide exchange factors (GEFs) are enzymes that promote the activation of GTPases through GTP loading. Whole exome sequencing has identified rare variants in GEFs that are associated with disease, demonstrating that GEFs play critical roles in human development. However, the consequences...

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Detalles Bibliográficos
Autores principales:	Blaise, Alyssa M, Corcoran, Ellen E, Wattenberg, Eve S, Zhang, Yan-Ling, Cottrell, Jeffrey R, Koleske, Anthony J
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2021
Materias:	Methods Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8789339/ https://www.ncbi.nlm.nih.gov/pubmed/35087952 http://dx.doi.org/10.1093/biomethods/bpab024

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