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Diagnostic performance of two serological assays for the detection of SARS-CoV-2 specific antibodies: surveillance after vaccination

Massive vaccination programs are being carried out to limit the SARS-CoV-2 pandemic that started in December 2019. Serological tests are of major importance as an indicator of circulation of the virus and to assess how vaccine-induced immunity progresses. An Enzyme-Linked Immunosorbent Assay (ELISA)...

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Autores principales: Fresco-Taboada, Alba, García-Durán, Marga, Aira, Cristina, López, Lissett, Sastre, Patricia, van der Hoek, Lia, van Gils, Marit J., Brouwer, Philip J.M., Sanders, Rogier W., Holzer, Barbara, Zimpernikc, Irene, López-Collazo, Eduardo, Muñoz, Patricia, Rueda, Paloma, Vela, Carmen
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8789399/
https://www.ncbi.nlm.nih.gov/pubmed/35218991
http://dx.doi.org/10.1016/j.diagmicrobio.2022.115650
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author Fresco-Taboada, Alba
García-Durán, Marga
Aira, Cristina
López, Lissett
Sastre, Patricia
van der Hoek, Lia
van Gils, Marit J.
Brouwer, Philip J.M.
Sanders, Rogier W.
Holzer, Barbara
Zimpernikc, Irene
López-Collazo, Eduardo
Muñoz, Patricia
Rueda, Paloma
Vela, Carmen
author_facet Fresco-Taboada, Alba
García-Durán, Marga
Aira, Cristina
López, Lissett
Sastre, Patricia
van der Hoek, Lia
van Gils, Marit J.
Brouwer, Philip J.M.
Sanders, Rogier W.
Holzer, Barbara
Zimpernikc, Irene
López-Collazo, Eduardo
Muñoz, Patricia
Rueda, Paloma
Vela, Carmen
author_sort Fresco-Taboada, Alba
collection PubMed
description Massive vaccination programs are being carried out to limit the SARS-CoV-2 pandemic that started in December 2019. Serological tests are of major importance as an indicator of circulation of the virus and to assess how vaccine-induced immunity progresses. An Enzyme-Linked Immunosorbent Assay (ELISA) and a Lateral Flow Assay (LFA) have been developed based on the SARS-CoV-2 recombinant Receptor Binding Domain (RBD) and the combination of Spike and Nucleoprotein, respectively. The validation with 1272 serum samples by comparison with INgezim COVID 19 DR showed good diagnostic performance (sensitivity: 93.2%-97.2%; specificity: 98.3%-99.3%) for detection of previous contact with SARS-CoV-2. Moreover, according to our results, these assays can help in the serosurveillance during and after vaccination, by detecting the humoral immune response as soon as 15 days postvaccination and identifying low-respondents. Hence, these tests could play a key role in the progression to a COVID-19 free world, helping to adjust future vaccination protocols.
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spelling pubmed-87893992022-01-26 Diagnostic performance of two serological assays for the detection of SARS-CoV-2 specific antibodies: surveillance after vaccination Fresco-Taboada, Alba García-Durán, Marga Aira, Cristina López, Lissett Sastre, Patricia van der Hoek, Lia van Gils, Marit J. Brouwer, Philip J.M. Sanders, Rogier W. Holzer, Barbara Zimpernikc, Irene López-Collazo, Eduardo Muñoz, Patricia Rueda, Paloma Vela, Carmen Diagn Microbiol Infect Dis Article Massive vaccination programs are being carried out to limit the SARS-CoV-2 pandemic that started in December 2019. Serological tests are of major importance as an indicator of circulation of the virus and to assess how vaccine-induced immunity progresses. An Enzyme-Linked Immunosorbent Assay (ELISA) and a Lateral Flow Assay (LFA) have been developed based on the SARS-CoV-2 recombinant Receptor Binding Domain (RBD) and the combination of Spike and Nucleoprotein, respectively. The validation with 1272 serum samples by comparison with INgezim COVID 19 DR showed good diagnostic performance (sensitivity: 93.2%-97.2%; specificity: 98.3%-99.3%) for detection of previous contact with SARS-CoV-2. Moreover, according to our results, these assays can help in the serosurveillance during and after vaccination, by detecting the humoral immune response as soon as 15 days postvaccination and identifying low-respondents. Hence, these tests could play a key role in the progression to a COVID-19 free world, helping to adjust future vaccination protocols. Elsevier Inc. 2022-04 2022-01-26 /pmc/articles/PMC8789399/ /pubmed/35218991 http://dx.doi.org/10.1016/j.diagmicrobio.2022.115650 Text en © 2022 Elsevier Inc. All rights reserved. Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Article
Fresco-Taboada, Alba
García-Durán, Marga
Aira, Cristina
López, Lissett
Sastre, Patricia
van der Hoek, Lia
van Gils, Marit J.
Brouwer, Philip J.M.
Sanders, Rogier W.
Holzer, Barbara
Zimpernikc, Irene
López-Collazo, Eduardo
Muñoz, Patricia
Rueda, Paloma
Vela, Carmen
Diagnostic performance of two serological assays for the detection of SARS-CoV-2 specific antibodies: surveillance after vaccination
title Diagnostic performance of two serological assays for the detection of SARS-CoV-2 specific antibodies: surveillance after vaccination
title_full Diagnostic performance of two serological assays for the detection of SARS-CoV-2 specific antibodies: surveillance after vaccination
title_fullStr Diagnostic performance of two serological assays for the detection of SARS-CoV-2 specific antibodies: surveillance after vaccination
title_full_unstemmed Diagnostic performance of two serological assays for the detection of SARS-CoV-2 specific antibodies: surveillance after vaccination
title_short Diagnostic performance of two serological assays for the detection of SARS-CoV-2 specific antibodies: surveillance after vaccination
title_sort diagnostic performance of two serological assays for the detection of sars-cov-2 specific antibodies: surveillance after vaccination
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8789399/
https://www.ncbi.nlm.nih.gov/pubmed/35218991
http://dx.doi.org/10.1016/j.diagmicrobio.2022.115650
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