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Circular RNA circGLIS3 promotes bladder cancer proliferation via the miR-1273f/SKP1/Cyclin D1 axis

Extensive research confirmed that circRNA can play a regulatory role in various stages of tumors by interacting with various molecules. Identifying the differentially expressed circRNA in bladder cancer and exploring its regulatory mechanism on bladder cancer progression are urgent. In this study, w...

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Autores principales: Wu, Shuilian, Yang, Jialei, Xu, Haotian, Wang, Xin, Zhang, Ruirui, Lu, Wenmin, Yang, Jie, Li, Xiaofei, Chen, Sixian, Zou, Yunfeng, Nan, Aruo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8789643/
https://www.ncbi.nlm.nih.gov/pubmed/33656636
http://dx.doi.org/10.1007/s10565-021-09591-3
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author Wu, Shuilian
Yang, Jialei
Xu, Haotian
Wang, Xin
Zhang, Ruirui
Lu, Wenmin
Yang, Jie
Li, Xiaofei
Chen, Sixian
Zou, Yunfeng
Nan, Aruo
author_facet Wu, Shuilian
Yang, Jialei
Xu, Haotian
Wang, Xin
Zhang, Ruirui
Lu, Wenmin
Yang, Jie
Li, Xiaofei
Chen, Sixian
Zou, Yunfeng
Nan, Aruo
author_sort Wu, Shuilian
collection PubMed
description Extensive research confirmed that circRNA can play a regulatory role in various stages of tumors by interacting with various molecules. Identifying the differentially expressed circRNA in bladder cancer and exploring its regulatory mechanism on bladder cancer progression are urgent. In this study, we screened out a circRNA-circGLIS3 with a significant upregulation trend in both bladder cancer tissues and cells. Bioinformatics prediction results showed that circGLIS3 may be involved in multiple tumor-related pathways. Function gain and loss experiments verified circGLIS3 can affect the proliferation, migration, and invasion of bladder cancer cells in vitro. Moreover, silencing circGLIS3 inhibited bladder cancer cell growth in vivo. Subsequent research results indicated circGLIS3 regulated the expression of cyclin D1, a cell cycle–related protein, and cell cycle progression. Mechanically, circGLIS3 upregulates the expression of SKP1 by adsorbing miR-1273f and then promotes cyclin D1 expression, ultimately promoting the proliferation of bladder cancer cells. In summary, our study indicates that circGLIS3 plays an oncogene role in the development of bladder cancer and has potential to be a candidate for bladder cancer. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10565-021-09591-3.
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spelling pubmed-87896432022-02-02 Circular RNA circGLIS3 promotes bladder cancer proliferation via the miR-1273f/SKP1/Cyclin D1 axis Wu, Shuilian Yang, Jialei Xu, Haotian Wang, Xin Zhang, Ruirui Lu, Wenmin Yang, Jie Li, Xiaofei Chen, Sixian Zou, Yunfeng Nan, Aruo Cell Biol Toxicol Original Article Extensive research confirmed that circRNA can play a regulatory role in various stages of tumors by interacting with various molecules. Identifying the differentially expressed circRNA in bladder cancer and exploring its regulatory mechanism on bladder cancer progression are urgent. In this study, we screened out a circRNA-circGLIS3 with a significant upregulation trend in both bladder cancer tissues and cells. Bioinformatics prediction results showed that circGLIS3 may be involved in multiple tumor-related pathways. Function gain and loss experiments verified circGLIS3 can affect the proliferation, migration, and invasion of bladder cancer cells in vitro. Moreover, silencing circGLIS3 inhibited bladder cancer cell growth in vivo. Subsequent research results indicated circGLIS3 regulated the expression of cyclin D1, a cell cycle–related protein, and cell cycle progression. Mechanically, circGLIS3 upregulates the expression of SKP1 by adsorbing miR-1273f and then promotes cyclin D1 expression, ultimately promoting the proliferation of bladder cancer cells. In summary, our study indicates that circGLIS3 plays an oncogene role in the development of bladder cancer and has potential to be a candidate for bladder cancer. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s10565-021-09591-3. Springer Netherlands 2021-03-03 2022 /pmc/articles/PMC8789643/ /pubmed/33656636 http://dx.doi.org/10.1007/s10565-021-09591-3 Text en © The Author(s) 2021 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Original Article
Wu, Shuilian
Yang, Jialei
Xu, Haotian
Wang, Xin
Zhang, Ruirui
Lu, Wenmin
Yang, Jie
Li, Xiaofei
Chen, Sixian
Zou, Yunfeng
Nan, Aruo
Circular RNA circGLIS3 promotes bladder cancer proliferation via the miR-1273f/SKP1/Cyclin D1 axis
title Circular RNA circGLIS3 promotes bladder cancer proliferation via the miR-1273f/SKP1/Cyclin D1 axis
title_full Circular RNA circGLIS3 promotes bladder cancer proliferation via the miR-1273f/SKP1/Cyclin D1 axis
title_fullStr Circular RNA circGLIS3 promotes bladder cancer proliferation via the miR-1273f/SKP1/Cyclin D1 axis
title_full_unstemmed Circular RNA circGLIS3 promotes bladder cancer proliferation via the miR-1273f/SKP1/Cyclin D1 axis
title_short Circular RNA circGLIS3 promotes bladder cancer proliferation via the miR-1273f/SKP1/Cyclin D1 axis
title_sort circular rna circglis3 promotes bladder cancer proliferation via the mir-1273f/skp1/cyclin d1 axis
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8789643/
https://www.ncbi.nlm.nih.gov/pubmed/33656636
http://dx.doi.org/10.1007/s10565-021-09591-3
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