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The promoted hydrolysis effect of cellulase with ultrasound treatment is reflected on the sonicated rather than native brown rice
Brown rice is nutritionally superior to polished white rice, as it maintains a large content of external bran that involves a series of bioactive compounds. However, the presence of bran also restricts water diffusion and results in adverse quality of brown rice. In this work, ultrasound conditions...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8789687/ https://www.ncbi.nlm.nih.gov/pubmed/35077963 http://dx.doi.org/10.1016/j.ultsonch.2022.105920 |
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author | Li, Hongyan Xu, Minghao Yao, Xu Wen, Yangyang Lu, Shiyi Wang, Jing Sun, Baoguo |
author_facet | Li, Hongyan Xu, Minghao Yao, Xu Wen, Yangyang Lu, Shiyi Wang, Jing Sun, Baoguo |
author_sort | Li, Hongyan |
collection | PubMed |
description | Brown rice is nutritionally superior to polished white rice, as it maintains a large content of external bran that involves a series of bioactive compounds. However, the presence of bran also restricts water diffusion and results in adverse quality of brown rice. In this work, ultrasound conditions were optimized for cellulase to improve its hydrolysis effect on rice bran, and combinations of enzymatic and ultrasound treatment in different manners were conducted on brown rice, to improve the textural attributes. The results showed significant improvements in the catalytic activity and efficiency of cellulase after ultrasonication at the optimal intensity of 1.67 W cm(−3) and duration of 30 min, with the conformational variation of cellulase observed from the fluorescence spectra and circular dichroism (CD). Despite the enhanced activity of ultrasonicated cellulase, it leaded to a similar rice surface morphology and a comparable amount of released glucose, and equivalent textural parameters of brown rice treated by native cellulase. However, for the pre-sonicated brown rice, the ultrasonicated cellulase showed a significantly higher hydrolysis capacity than the untreated enzyme, suggesting the important influence of ruptured bran surface on amplifying the hydrolysis effect of cellulase. Compared to the successive ultrasound stimulation on both cellulase and brown rice, ultrasound-assisted cellulase treatment on brown rice produced less glucose from rice bran, but induced similar textural properties of brown rice, possibly resulting from the simultaneously promoting effect of ultrasonication on cellulase and water diffusion. Ultimately, this study highlighted that the mild rice surface rupture is a crucial factor to display the promoted hydrolysis effect of ultrasonicated cellulase on brown rice. Ultrasound-assisted cellulase treatment potentially provides an effective strategy to improve the edible quality of brown rice. |
format | Online Article Text |
id | pubmed-8789687 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-87896872022-02-01 The promoted hydrolysis effect of cellulase with ultrasound treatment is reflected on the sonicated rather than native brown rice Li, Hongyan Xu, Minghao Yao, Xu Wen, Yangyang Lu, Shiyi Wang, Jing Sun, Baoguo Ultrason Sonochem Short Communication Brown rice is nutritionally superior to polished white rice, as it maintains a large content of external bran that involves a series of bioactive compounds. However, the presence of bran also restricts water diffusion and results in adverse quality of brown rice. In this work, ultrasound conditions were optimized for cellulase to improve its hydrolysis effect on rice bran, and combinations of enzymatic and ultrasound treatment in different manners were conducted on brown rice, to improve the textural attributes. The results showed significant improvements in the catalytic activity and efficiency of cellulase after ultrasonication at the optimal intensity of 1.67 W cm(−3) and duration of 30 min, with the conformational variation of cellulase observed from the fluorescence spectra and circular dichroism (CD). Despite the enhanced activity of ultrasonicated cellulase, it leaded to a similar rice surface morphology and a comparable amount of released glucose, and equivalent textural parameters of brown rice treated by native cellulase. However, for the pre-sonicated brown rice, the ultrasonicated cellulase showed a significantly higher hydrolysis capacity than the untreated enzyme, suggesting the important influence of ruptured bran surface on amplifying the hydrolysis effect of cellulase. Compared to the successive ultrasound stimulation on both cellulase and brown rice, ultrasound-assisted cellulase treatment on brown rice produced less glucose from rice bran, but induced similar textural properties of brown rice, possibly resulting from the simultaneously promoting effect of ultrasonication on cellulase and water diffusion. Ultimately, this study highlighted that the mild rice surface rupture is a crucial factor to display the promoted hydrolysis effect of ultrasonicated cellulase on brown rice. Ultrasound-assisted cellulase treatment potentially provides an effective strategy to improve the edible quality of brown rice. Elsevier 2022-01-19 /pmc/articles/PMC8789687/ /pubmed/35077963 http://dx.doi.org/10.1016/j.ultsonch.2022.105920 Text en © 2022 The Authors. Published by Elsevier B.V. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Short Communication Li, Hongyan Xu, Minghao Yao, Xu Wen, Yangyang Lu, Shiyi Wang, Jing Sun, Baoguo The promoted hydrolysis effect of cellulase with ultrasound treatment is reflected on the sonicated rather than native brown rice |
title | The promoted hydrolysis effect of cellulase with ultrasound treatment is reflected on the sonicated rather than native brown rice |
title_full | The promoted hydrolysis effect of cellulase with ultrasound treatment is reflected on the sonicated rather than native brown rice |
title_fullStr | The promoted hydrolysis effect of cellulase with ultrasound treatment is reflected on the sonicated rather than native brown rice |
title_full_unstemmed | The promoted hydrolysis effect of cellulase with ultrasound treatment is reflected on the sonicated rather than native brown rice |
title_short | The promoted hydrolysis effect of cellulase with ultrasound treatment is reflected on the sonicated rather than native brown rice |
title_sort | promoted hydrolysis effect of cellulase with ultrasound treatment is reflected on the sonicated rather than native brown rice |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8789687/ https://www.ncbi.nlm.nih.gov/pubmed/35077963 http://dx.doi.org/10.1016/j.ultsonch.2022.105920 |
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