Improved gRNA secondary structures allow editing of target sites resistant to CRISPR-Cas9 cleavage

The first step in CRISPR-Cas9-mediated genome editing is the cleavage of target DNA sequences that are complementary to so-called spacer sequences in CRISPR guide RNAs (gRNAs). However, some DNA sequences are refractory to CRISPR-Cas9 cleavage, which is at least in part due to gRNA misfolding. To ov...

Descripción completa

Detalles Bibliográficos
Autores principales: Riesenberg, Stephan, Helmbrecht, Nelly, Kanis, Philipp, Maricic, Tomislav, Pääbo, Svante
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8789806/
https://www.ncbi.nlm.nih.gov/pubmed/35078986
http://dx.doi.org/10.1038/s41467-022-28137-7

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8789806/
https://www.ncbi.nlm.nih.gov/pubmed/35078986
http://dx.doi.org/10.1038/s41467-022-28137-7

Ejemplares similares