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Tracing PRX1(+) cells during molar formation and periodontal ligament reconstruction

Neural crest-derived mesenchymal stem cells (MSCs) are known to play an essential function during tooth and skeletal development. PRX1(+) cells constitute an important MSC subtype that is implicated in osteogenesis. However, their potential function in tooth development and regeneration remains elus...

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Autores principales: Gong, Xuyan, Zhang, Han, Xu, Xiaoqiao, Ding, Yunpeng, Yang, Xingbo, Cheng, Zhiyang, Tao, Dike, Hu, Congjiao, Xiang, Yaozu, Sun, Yao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8789835/
https://www.ncbi.nlm.nih.gov/pubmed/35078971
http://dx.doi.org/10.1038/s41368-021-00155-z
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author Gong, Xuyan
Zhang, Han
Xu, Xiaoqiao
Ding, Yunpeng
Yang, Xingbo
Cheng, Zhiyang
Tao, Dike
Hu, Congjiao
Xiang, Yaozu
Sun, Yao
author_facet Gong, Xuyan
Zhang, Han
Xu, Xiaoqiao
Ding, Yunpeng
Yang, Xingbo
Cheng, Zhiyang
Tao, Dike
Hu, Congjiao
Xiang, Yaozu
Sun, Yao
author_sort Gong, Xuyan
collection PubMed
description Neural crest-derived mesenchymal stem cells (MSCs) are known to play an essential function during tooth and skeletal development. PRX1(+) cells constitute an important MSC subtype that is implicated in osteogenesis. However, their potential function in tooth development and regeneration remains elusive. In the present study, we first assessed the cell fate of PRX1(+) cells during molar development and periodontal ligament (PDL) formation in mice. Furthermore, single-cell RNA sequencing analysis was performed to study the distribution of PRX1(+) cells in PDL cells. The behavior of PRX1(+) cells during PDL reconstruction was investigated using an allogeneic transplanted tooth model. Although PRX1(+) cells are spatial specific and can differentiate into almost all types of mesenchymal cells in first molars, their distribution in third molars is highly limited. The PDL formation is associated with a high number of PRX1(+) cells; during transplanted teeth PDL reconstruction, PRX1(+) cells from the recipient alveolar bone participate in angiogenesis as pericytes. Overall, PRX1(+) cells are a key subtype of dental MSCs involved in the formation of mouse molar and PDL and participate in angiogenesis as pericytes during PDL reconstruction after tooth transplantation.
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spelling pubmed-87898352022-02-07 Tracing PRX1(+) cells during molar formation and periodontal ligament reconstruction Gong, Xuyan Zhang, Han Xu, Xiaoqiao Ding, Yunpeng Yang, Xingbo Cheng, Zhiyang Tao, Dike Hu, Congjiao Xiang, Yaozu Sun, Yao Int J Oral Sci Article Neural crest-derived mesenchymal stem cells (MSCs) are known to play an essential function during tooth and skeletal development. PRX1(+) cells constitute an important MSC subtype that is implicated in osteogenesis. However, their potential function in tooth development and regeneration remains elusive. In the present study, we first assessed the cell fate of PRX1(+) cells during molar development and periodontal ligament (PDL) formation in mice. Furthermore, single-cell RNA sequencing analysis was performed to study the distribution of PRX1(+) cells in PDL cells. The behavior of PRX1(+) cells during PDL reconstruction was investigated using an allogeneic transplanted tooth model. Although PRX1(+) cells are spatial specific and can differentiate into almost all types of mesenchymal cells in first molars, their distribution in third molars is highly limited. The PDL formation is associated with a high number of PRX1(+) cells; during transplanted teeth PDL reconstruction, PRX1(+) cells from the recipient alveolar bone participate in angiogenesis as pericytes. Overall, PRX1(+) cells are a key subtype of dental MSCs involved in the formation of mouse molar and PDL and participate in angiogenesis as pericytes during PDL reconstruction after tooth transplantation. Nature Publishing Group UK 2022-01-25 /pmc/articles/PMC8789835/ /pubmed/35078971 http://dx.doi.org/10.1038/s41368-021-00155-z Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Gong, Xuyan
Zhang, Han
Xu, Xiaoqiao
Ding, Yunpeng
Yang, Xingbo
Cheng, Zhiyang
Tao, Dike
Hu, Congjiao
Xiang, Yaozu
Sun, Yao
Tracing PRX1(+) cells during molar formation and periodontal ligament reconstruction
title Tracing PRX1(+) cells during molar formation and periodontal ligament reconstruction
title_full Tracing PRX1(+) cells during molar formation and periodontal ligament reconstruction
title_fullStr Tracing PRX1(+) cells during molar formation and periodontal ligament reconstruction
title_full_unstemmed Tracing PRX1(+) cells during molar formation and periodontal ligament reconstruction
title_short Tracing PRX1(+) cells during molar formation and periodontal ligament reconstruction
title_sort tracing prx1(+) cells during molar formation and periodontal ligament reconstruction
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8789835/
https://www.ncbi.nlm.nih.gov/pubmed/35078971
http://dx.doi.org/10.1038/s41368-021-00155-z
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