Dual in Utero Electroporation in Mice to Manipulate Two Specific Neuronal Populations in the Developing Cortex

Precise regulation of gene expression during development in cortical neurons is essential for the establishment and maintenance of neuronal connectivity and higher-order cognition. Dual in utero electroporation provides a precise and effective tool to label and manipulate gene expression in multiple...

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Autores principales: Zhang, Longbo, Getz, Stephanie A., Bordey, Angelique
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Bioengineering and Biotechnology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8790278/
https://www.ncbi.nlm.nih.gov/pubmed/35096799
http://dx.doi.org/10.3389/fbioe.2021.814638
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author Zhang, Longbo
Getz, Stephanie A.
Bordey, Angelique
author_facet Zhang, Longbo
Getz, Stephanie A.
Bordey, Angelique
author_sort Zhang, Longbo
collection PubMed
description Precise regulation of gene expression during development in cortical neurons is essential for the establishment and maintenance of neuronal connectivity and higher-order cognition. Dual in utero electroporation provides a precise and effective tool to label and manipulate gene expression in multiple neuronal populations within a circuit in a spatially and temporally regulated manner. In addition, this technique allows for morphophysiological investigations into neuronal development and connectivity following cell-specific gene manipulations. Here, we detail the dual in utero electroporation protocol.
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spelling pubmed-87902782022-01-27 Dual in Utero Electroporation in Mice to Manipulate Two Specific Neuronal Populations in the Developing Cortex Zhang, Longbo Getz, Stephanie A. Bordey, Angelique Front Bioeng Biotechnol Bioengineering and Biotechnology Precise regulation of gene expression during development in cortical neurons is essential for the establishment and maintenance of neuronal connectivity and higher-order cognition. Dual in utero electroporation provides a precise and effective tool to label and manipulate gene expression in multiple neuronal populations within a circuit in a spatially and temporally regulated manner. In addition, this technique allows for morphophysiological investigations into neuronal development and connectivity following cell-specific gene manipulations. Here, we detail the dual in utero electroporation protocol. Frontiers Media S.A. 2022-01-12 /pmc/articles/PMC8790278/ /pubmed/35096799 http://dx.doi.org/10.3389/fbioe.2021.814638 Text en Copyright © 2022 Zhang, Getz and Bordey. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Zhang, Longbo
Getz, Stephanie A.
Bordey, Angelique
Dual in Utero Electroporation in Mice to Manipulate Two Specific Neuronal Populations in the Developing Cortex
title Dual in Utero Electroporation in Mice to Manipulate Two Specific Neuronal Populations in the Developing Cortex
title_full Dual in Utero Electroporation in Mice to Manipulate Two Specific Neuronal Populations in the Developing Cortex
title_fullStr Dual in Utero Electroporation in Mice to Manipulate Two Specific Neuronal Populations in the Developing Cortex
title_full_unstemmed Dual in Utero Electroporation in Mice to Manipulate Two Specific Neuronal Populations in the Developing Cortex
title_short Dual in Utero Electroporation in Mice to Manipulate Two Specific Neuronal Populations in the Developing Cortex
title_sort dual in utero electroporation in mice to manipulate two specific neuronal populations in the developing cortex
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8790278/
https://www.ncbi.nlm.nih.gov/pubmed/35096799
http://dx.doi.org/10.3389/fbioe.2021.814638
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AT bordeyangelique dualinuteroelectroporationinmicetomanipulatetwospecificneuronalpopulationsinthedevelopingcortex

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