Efficiency, Specificity and Temperature Sensitivity of Cas9 and Cas12a RNPs for DNA-free Genome Editing in Plants

Delivery of genome editing reagents using CRISPR-Cas ribonucleoproteins (RNPs) transfection offers several advantages over plasmid DNA-based delivery methods, including reduced off-target editing effects, mitigation of random integration of non-native DNA fragments, independence of vector constructi...

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Detalles Bibliográficos
Autores principales: Banakar, Raviraj, Schubert, Mollie, Kurgan, Gavin, Rai, Krishan Mohan, Beaudoin, Sarah F., Collingwood, Michael A., Vakulskas, Christopher A., Wang, Kan, Zhang, Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Genome Editing
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8790294/
https://www.ncbi.nlm.nih.gov/pubmed/35098208
http://dx.doi.org/10.3389/fgeed.2021.760820

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8790294/
https://www.ncbi.nlm.nih.gov/pubmed/35098208
http://dx.doi.org/10.3389/fgeed.2021.760820

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