Orthogonal protocols for DNA extraction from filamentous fungi

There are few protocols available for DNA extraction from fungi. Here we present four complementary protocols for extraction of genomic DNA from fungi. We quantify the efficacy of extractions and compare eight species from five filamentous fungal genera, including both basidiomycetes and ascomycetes...

Descripción completa

Detalles Bibliográficos
Autores principales: Conlon, Benjamin H., Schmidt, Suzanne, Poulsen, Michael, Shik, Jonathan Z.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8790498/
https://www.ncbi.nlm.nih.gov/pubmed/35112085
http://dx.doi.org/10.1016/j.xpro.2022.101126
Descripción
Sumario:There are few protocols available for DNA extraction from fungi. Here we present four complementary protocols for extraction of genomic DNA from fungi. We quantify the efficacy of extractions and compare eight species from five filamentous fungal genera, including both basidiomycetes and ascomycetes. These protocols should be useful for extraction of DNA from a variety of filamentous fungi. For complete details on the use and execution of this protocol, please refer to Conlon et al. (2021).

Ejemplares similares