Orthogonal protocols for DNA extraction from filamentous fungi

There are few protocols available for DNA extraction from fungi. Here we present four complementary protocols for extraction of genomic DNA from fungi. We quantify the efficacy of extractions and compare eight species from five filamentous fungal genera, including both basidiomycetes and ascomycetes...

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Detalles Bibliográficos
Autores principales: Conlon, Benjamin H., Schmidt, Suzanne, Poulsen, Michael, Shik, Jonathan Z.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8790498/
https://www.ncbi.nlm.nih.gov/pubmed/35112085
http://dx.doi.org/10.1016/j.xpro.2022.101126
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author Conlon, Benjamin H.
Schmidt, Suzanne
Poulsen, Michael
Shik, Jonathan Z.
author_facet Conlon, Benjamin H.
Schmidt, Suzanne
Poulsen, Michael
Shik, Jonathan Z.
author_sort Conlon, Benjamin H.
collection PubMed
description There are few protocols available for DNA extraction from fungi. Here we present four complementary protocols for extraction of genomic DNA from fungi. We quantify the efficacy of extractions and compare eight species from five filamentous fungal genera, including both basidiomycetes and ascomycetes. These protocols should be useful for extraction of DNA from a variety of filamentous fungi. For complete details on the use and execution of this protocol, please refer to Conlon et al. (2021).
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spelling pubmed-87904982022-02-01 Orthogonal protocols for DNA extraction from filamentous fungi Conlon, Benjamin H. Schmidt, Suzanne Poulsen, Michael Shik, Jonathan Z. STAR Protoc Protocol There are few protocols available for DNA extraction from fungi. Here we present four complementary protocols for extraction of genomic DNA from fungi. We quantify the efficacy of extractions and compare eight species from five filamentous fungal genera, including both basidiomycetes and ascomycetes. These protocols should be useful for extraction of DNA from a variety of filamentous fungi. For complete details on the use and execution of this protocol, please refer to Conlon et al. (2021). Elsevier 2022-01-24 /pmc/articles/PMC8790498/ /pubmed/35112085 http://dx.doi.org/10.1016/j.xpro.2022.101126 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Conlon, Benjamin H.
Schmidt, Suzanne
Poulsen, Michael
Shik, Jonathan Z.
Orthogonal protocols for DNA extraction from filamentous fungi
title Orthogonal protocols for DNA extraction from filamentous fungi
title_full Orthogonal protocols for DNA extraction from filamentous fungi
title_fullStr Orthogonal protocols for DNA extraction from filamentous fungi
title_full_unstemmed Orthogonal protocols for DNA extraction from filamentous fungi
title_short Orthogonal protocols for DNA extraction from filamentous fungi
title_sort orthogonal protocols for dna extraction from filamentous fungi
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8790498/
https://www.ncbi.nlm.nih.gov/pubmed/35112085
http://dx.doi.org/10.1016/j.xpro.2022.101126
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