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Cryopreservation and post-thaw characterization of dissociated human islet cells

The objective of this study is to optimize the cryopreservation of dissociated islet cells and obtain functional cells that can be used in single-cell transcriptome studies on the pathology and treatment of diabetes. Using an iterative graded freezing approach we obtained viable cells after cooling...

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Detalles Bibliográficos
Autores principales:	Marquez-Curtis, Leah A., Dai, Xiao-Qing, Hang, Yan, Lam, Jonathan Y., Lyon, James, Manning Fox, Jocelyn E., McGann, Locksley E., MacDonald, Patrick E., Kim, Seung K., Elliott, Janet A. W.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2022
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8791532/ https://www.ncbi.nlm.nih.gov/pubmed/35081145 http://dx.doi.org/10.1371/journal.pone.0263005

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