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Detection of Gag C-terminal mutations among HIV-1 non-B subtypes in a subset of Cameroonian patients

Response to ritonavir-boosted-protease inhibitors (PI/r)-based regimen is associated with some Gag mutations among HIV-1 B-clade. There is limited data on Gag mutations and their covariation with mutations in protease among HIV-1 non-B-clades at PI/r-based treatment failure. Thus, we characterized G...

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Autores principales: Teto, Georges, Nka, Alex Durand, Fokam, Joseph, Bouba, Yagai, Takou, Désiré, Fabeni, Lavinia, Carioti, Luca, Armenia, Daniele, Semengue, Ezéchiel Ngoufack Jagni, Dambaya, Béatrice, Sosso, Samuel Martin, Colizzi, Vittorio, Perno, Carlo-Federico, Ceccherini-Silberstein, Francesca, Santoro, Maria Mercedes, Ndjolo, Alexis
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8791941/
https://www.ncbi.nlm.nih.gov/pubmed/35082353
http://dx.doi.org/10.1038/s41598-022-05375-9
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author Teto, Georges
Nka, Alex Durand
Fokam, Joseph
Bouba, Yagai
Takou, Désiré
Fabeni, Lavinia
Carioti, Luca
Armenia, Daniele
Semengue, Ezéchiel Ngoufack Jagni
Dambaya, Béatrice
Sosso, Samuel Martin
Colizzi, Vittorio
Perno, Carlo-Federico
Ceccherini-Silberstein, Francesca
Santoro, Maria Mercedes
Ndjolo, Alexis
author_facet Teto, Georges
Nka, Alex Durand
Fokam, Joseph
Bouba, Yagai
Takou, Désiré
Fabeni, Lavinia
Carioti, Luca
Armenia, Daniele
Semengue, Ezéchiel Ngoufack Jagni
Dambaya, Béatrice
Sosso, Samuel Martin
Colizzi, Vittorio
Perno, Carlo-Federico
Ceccherini-Silberstein, Francesca
Santoro, Maria Mercedes
Ndjolo, Alexis
author_sort Teto, Georges
collection PubMed
description Response to ritonavir-boosted-protease inhibitors (PI/r)-based regimen is associated with some Gag mutations among HIV-1 B-clade. There is limited data on Gag mutations and their covariation with mutations in protease among HIV-1 non-B-clades at PI/r-based treatment failure. Thus, we characterized Gag mutations present in isolates from HIV-1 infected individuals treated with a PI/r-regimen (n = 143) and compared them with those obtained from individuals not treated with PI/r (ART-naïve [n = 101] or reverse transcriptase inhibitors (RTI) treated [n = 118]). The most frequent HIV-1 subtypes were CRF02_AG (54.69%), A (13.53%), D (6.35%) and G (4.69%). Eighteen Gag mutations showed a significantly higher prevalence in PI/r-treated isolates compared to ART-naïve (p < 0.05): Group 1 (prevalence < 1% in drug-naïve): L449F, D480N, L483Q, Y484P, T487V; group 2 (prevalence 1–5% in drug-naïve): S462L, I479G, I479K, D480E; group 3 (prevalence ≥ 5% in drug-naïve): P453L, E460A, R464G, S465F, V467E, Q474P, I479R, E482G, T487A. Five Gag mutations (L449F, P453L, D480E, S465F, Y484P) positively correlated (Phi ≥ 0.2, p < 0.05) with protease-resistance mutations. At PI/r-failure, no significant difference was observed between patients with and without these associated Gag mutations in term of viremia or CD4 count. This analysis suggests that some Gag mutations show an increased frequency in patients failing PIs among HIV-1 non-B clades.
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spelling pubmed-87919412022-01-27 Detection of Gag C-terminal mutations among HIV-1 non-B subtypes in a subset of Cameroonian patients Teto, Georges Nka, Alex Durand Fokam, Joseph Bouba, Yagai Takou, Désiré Fabeni, Lavinia Carioti, Luca Armenia, Daniele Semengue, Ezéchiel Ngoufack Jagni Dambaya, Béatrice Sosso, Samuel Martin Colizzi, Vittorio Perno, Carlo-Federico Ceccherini-Silberstein, Francesca Santoro, Maria Mercedes Ndjolo, Alexis Sci Rep Article Response to ritonavir-boosted-protease inhibitors (PI/r)-based regimen is associated with some Gag mutations among HIV-1 B-clade. There is limited data on Gag mutations and their covariation with mutations in protease among HIV-1 non-B-clades at PI/r-based treatment failure. Thus, we characterized Gag mutations present in isolates from HIV-1 infected individuals treated with a PI/r-regimen (n = 143) and compared them with those obtained from individuals not treated with PI/r (ART-naïve [n = 101] or reverse transcriptase inhibitors (RTI) treated [n = 118]). The most frequent HIV-1 subtypes were CRF02_AG (54.69%), A (13.53%), D (6.35%) and G (4.69%). Eighteen Gag mutations showed a significantly higher prevalence in PI/r-treated isolates compared to ART-naïve (p < 0.05): Group 1 (prevalence < 1% in drug-naïve): L449F, D480N, L483Q, Y484P, T487V; group 2 (prevalence 1–5% in drug-naïve): S462L, I479G, I479K, D480E; group 3 (prevalence ≥ 5% in drug-naïve): P453L, E460A, R464G, S465F, V467E, Q474P, I479R, E482G, T487A. Five Gag mutations (L449F, P453L, D480E, S465F, Y484P) positively correlated (Phi ≥ 0.2, p < 0.05) with protease-resistance mutations. At PI/r-failure, no significant difference was observed between patients with and without these associated Gag mutations in term of viremia or CD4 count. This analysis suggests that some Gag mutations show an increased frequency in patients failing PIs among HIV-1 non-B clades. Nature Publishing Group UK 2022-01-26 /pmc/articles/PMC8791941/ /pubmed/35082353 http://dx.doi.org/10.1038/s41598-022-05375-9 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Teto, Georges
Nka, Alex Durand
Fokam, Joseph
Bouba, Yagai
Takou, Désiré
Fabeni, Lavinia
Carioti, Luca
Armenia, Daniele
Semengue, Ezéchiel Ngoufack Jagni
Dambaya, Béatrice
Sosso, Samuel Martin
Colizzi, Vittorio
Perno, Carlo-Federico
Ceccherini-Silberstein, Francesca
Santoro, Maria Mercedes
Ndjolo, Alexis
Detection of Gag C-terminal mutations among HIV-1 non-B subtypes in a subset of Cameroonian patients
title Detection of Gag C-terminal mutations among HIV-1 non-B subtypes in a subset of Cameroonian patients
title_full Detection of Gag C-terminal mutations among HIV-1 non-B subtypes in a subset of Cameroonian patients
title_fullStr Detection of Gag C-terminal mutations among HIV-1 non-B subtypes in a subset of Cameroonian patients
title_full_unstemmed Detection of Gag C-terminal mutations among HIV-1 non-B subtypes in a subset of Cameroonian patients
title_short Detection of Gag C-terminal mutations among HIV-1 non-B subtypes in a subset of Cameroonian patients
title_sort detection of gag c-terminal mutations among hiv-1 non-b subtypes in a subset of cameroonian patients
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8791941/
https://www.ncbi.nlm.nih.gov/pubmed/35082353
http://dx.doi.org/10.1038/s41598-022-05375-9
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