A protocol for quantifying lymphocyte-mediated cytotoxicity using an impedance-based real-time cell analyzer

Current standard assays to analyze lymphocyte-mediated antitumor cytotoxicity employ radioisotopic or fluorescent labels. However, such assays are not suitable for real-time analysis. Here we describe a protocol that facilitates the analysis of lymphocyte-mediated toxicity using a label-free, impedance-based real-time cell analyzer. This analyzer measures cellular electrical impedance, expressed as the cell index value, noninvasively and continuously. In contrast with label-dependent assays, this protocol simultaneously generates real-time killing curves useful for quantifying lymphocyte-mediated cytotoxicity in real time. For complete details on the use and execution of this protocol, please refer to Kanemaru et al. (2021).