A protocol for quantifying lymphocyte-mediated cytotoxicity using an impedance-based real-time cell analyzer

Current standard assays to analyze lymphocyte-mediated antitumor cytotoxicity employ radioisotopic or fluorescent labels. However, such assays are not suitable for real-time analysis. Here we describe a protocol that facilitates the analysis of lymphocyte-mediated toxicity using a label-free, impeda...

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Autores principales: Kanemaru, Hisashi, Mizukami, Yukari, Kaneko, Akira, Kajihara, Ikko, Fukushima, Satoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8792266/
https://www.ncbi.nlm.nih.gov/pubmed/35118432
http://dx.doi.org/10.1016/j.xpro.2022.101128
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author Kanemaru, Hisashi
Mizukami, Yukari
Kaneko, Akira
Kajihara, Ikko
Fukushima, Satoshi
author_facet Kanemaru, Hisashi
Mizukami, Yukari
Kaneko, Akira
Kajihara, Ikko
Fukushima, Satoshi
author_sort Kanemaru, Hisashi
collection PubMed
description Current standard assays to analyze lymphocyte-mediated antitumor cytotoxicity employ radioisotopic or fluorescent labels. However, such assays are not suitable for real-time analysis. Here we describe a protocol that facilitates the analysis of lymphocyte-mediated toxicity using a label-free, impedance-based real-time cell analyzer. This analyzer measures cellular electrical impedance, expressed as the cell index value, noninvasively and continuously. In contrast with label-dependent assays, this protocol simultaneously generates real-time killing curves useful for quantifying lymphocyte-mediated cytotoxicity in real time. For complete details on the use and execution of this protocol, please refer to Kanemaru et al. (2021).
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spelling pubmed-87922662022-02-02 A protocol for quantifying lymphocyte-mediated cytotoxicity using an impedance-based real-time cell analyzer Kanemaru, Hisashi Mizukami, Yukari Kaneko, Akira Kajihara, Ikko Fukushima, Satoshi STAR Protoc Protocol Current standard assays to analyze lymphocyte-mediated antitumor cytotoxicity employ radioisotopic or fluorescent labels. However, such assays are not suitable for real-time analysis. Here we describe a protocol that facilitates the analysis of lymphocyte-mediated toxicity using a label-free, impedance-based real-time cell analyzer. This analyzer measures cellular electrical impedance, expressed as the cell index value, noninvasively and continuously. In contrast with label-dependent assays, this protocol simultaneously generates real-time killing curves useful for quantifying lymphocyte-mediated cytotoxicity in real time. For complete details on the use and execution of this protocol, please refer to Kanemaru et al. (2021). Elsevier 2022-01-22 /pmc/articles/PMC8792266/ /pubmed/35118432 http://dx.doi.org/10.1016/j.xpro.2022.101128 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Kanemaru, Hisashi
Mizukami, Yukari
Kaneko, Akira
Kajihara, Ikko
Fukushima, Satoshi
A protocol for quantifying lymphocyte-mediated cytotoxicity using an impedance-based real-time cell analyzer
title A protocol for quantifying lymphocyte-mediated cytotoxicity using an impedance-based real-time cell analyzer
title_full A protocol for quantifying lymphocyte-mediated cytotoxicity using an impedance-based real-time cell analyzer
title_fullStr A protocol for quantifying lymphocyte-mediated cytotoxicity using an impedance-based real-time cell analyzer
title_full_unstemmed A protocol for quantifying lymphocyte-mediated cytotoxicity using an impedance-based real-time cell analyzer
title_short A protocol for quantifying lymphocyte-mediated cytotoxicity using an impedance-based real-time cell analyzer
title_sort protocol for quantifying lymphocyte-mediated cytotoxicity using an impedance-based real-time cell analyzer
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8792266/
https://www.ncbi.nlm.nih.gov/pubmed/35118432
http://dx.doi.org/10.1016/j.xpro.2022.101128
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