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Metabolic labelling of cancer cells with glycodendrimers stimulate immune-mediated cytotoxicity
The recruitment of antibody naturally present in human blood stream at the surface of cancer cells have been proved a promising immunotherapeutic strategy to fight cancer. Antibody recruiting molecules (ARMs) combining tumor and antibody binding modules have been developed for this purpose, however...
Autores principales: | , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
RSC
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8792828/ https://www.ncbi.nlm.nih.gov/pubmed/35211675 http://dx.doi.org/10.1039/d1md00262g |
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author | Goyard, David Diriwari, Peremobowei Iyanu Berthet, Nathalie |
author_facet | Goyard, David Diriwari, Peremobowei Iyanu Berthet, Nathalie |
author_sort | Goyard, David |
collection | PubMed |
description | The recruitment of antibody naturally present in human blood stream at the surface of cancer cells have been proved a promising immunotherapeutic strategy to fight cancer. Antibody recruiting molecules (ARMs) combining tumor and antibody binding modules have been developed for this purpose, however the formation of the interacting complex with both antibody and cell is difficult to optimize to stimulate immune-mediated cytotoxicity. To circumvent this limitation, we report herein a more direct approach combining cell metabolism of azido-sugar and bio-orthogonal click chemistry to conjugate at the cell glycocalyx structurally well-defined glycodendrimers as antibody binding module (ABM). We demonstrate that this strategy allows not only the recruitment of natural antibody at the surface of isolated cells or solid tumor models but also activate a cytotoxic response with human serum as unique source of immune effectors. |
format | Online Article Text |
id | pubmed-8792828 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | RSC |
record_format | MEDLINE/PubMed |
spelling | pubmed-87928282022-02-23 Metabolic labelling of cancer cells with glycodendrimers stimulate immune-mediated cytotoxicity Goyard, David Diriwari, Peremobowei Iyanu Berthet, Nathalie RSC Med Chem Chemistry The recruitment of antibody naturally present in human blood stream at the surface of cancer cells have been proved a promising immunotherapeutic strategy to fight cancer. Antibody recruiting molecules (ARMs) combining tumor and antibody binding modules have been developed for this purpose, however the formation of the interacting complex with both antibody and cell is difficult to optimize to stimulate immune-mediated cytotoxicity. To circumvent this limitation, we report herein a more direct approach combining cell metabolism of azido-sugar and bio-orthogonal click chemistry to conjugate at the cell glycocalyx structurally well-defined glycodendrimers as antibody binding module (ABM). We demonstrate that this strategy allows not only the recruitment of natural antibody at the surface of isolated cells or solid tumor models but also activate a cytotoxic response with human serum as unique source of immune effectors. RSC 2021-11-09 /pmc/articles/PMC8792828/ /pubmed/35211675 http://dx.doi.org/10.1039/d1md00262g Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ |
spellingShingle | Chemistry Goyard, David Diriwari, Peremobowei Iyanu Berthet, Nathalie Metabolic labelling of cancer cells with glycodendrimers stimulate immune-mediated cytotoxicity |
title | Metabolic labelling of cancer cells with glycodendrimers stimulate immune-mediated cytotoxicity |
title_full | Metabolic labelling of cancer cells with glycodendrimers stimulate immune-mediated cytotoxicity |
title_fullStr | Metabolic labelling of cancer cells with glycodendrimers stimulate immune-mediated cytotoxicity |
title_full_unstemmed | Metabolic labelling of cancer cells with glycodendrimers stimulate immune-mediated cytotoxicity |
title_short | Metabolic labelling of cancer cells with glycodendrimers stimulate immune-mediated cytotoxicity |
title_sort | metabolic labelling of cancer cells with glycodendrimers stimulate immune-mediated cytotoxicity |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8792828/ https://www.ncbi.nlm.nih.gov/pubmed/35211675 http://dx.doi.org/10.1039/d1md00262g |
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