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Comparison of Extended-Spectrum Beta-Lactamase-Producing Escherichia coli Isolates From Rooks (Corvus frugilegus) and Contemporary Human-Derived Strains: A One Health Perspective
During winter, a large number of rooks gather and defecate at the park of a university clinic. We investigated the prevalence of extended-spectrum beta-lactamase (ESBL)–producing Escherichia coli in these birds and compared recovered isolates with contemporary human isolates. In 2016, fecal samples...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8792927/ https://www.ncbi.nlm.nih.gov/pubmed/35095799 http://dx.doi.org/10.3389/fmicb.2021.785411 |
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author | Nagy, Bálint József Balázs, Bence Benmazouz, Isma Gyüre, Péter Kövér, László Kaszab, Eszter Bali, Krisztina Lovas-Kiss, Ádám Damjanova, Ivelina Majoros, László Tóth, Ákos Bányai, Krisztián Kardos, Gábor |
author_facet | Nagy, Bálint József Balázs, Bence Benmazouz, Isma Gyüre, Péter Kövér, László Kaszab, Eszter Bali, Krisztina Lovas-Kiss, Ádám Damjanova, Ivelina Majoros, László Tóth, Ákos Bányai, Krisztián Kardos, Gábor |
author_sort | Nagy, Bálint József |
collection | PubMed |
description | During winter, a large number of rooks gather and defecate at the park of a university clinic. We investigated the prevalence of extended-spectrum beta-lactamase (ESBL)–producing Escherichia coli in these birds and compared recovered isolates with contemporary human isolates. In 2016, fecal samples were collected from 112 trap-captured rooks and investigated for presence of ESBL producers using eosin methylene blue agar supplemented by 2 mg/L cefotaxime; 2,455 contemporary human fecal samples of patients of the clinics sent for routine culturing were tested similarly. In addition, 42 ESBL-producing E. coli isolates collected during the same period from inpatients were also studied. ESBL genes were sought for by PCR and were characterized by sequencing; E. coli ST131 clones were identified. Epidemiological relatedness was determined by pulsed-field gel electrophoresis and confirmed using whole genome sequencing in selected cases. Thirty-seven (33%) of sampled rooks and 42 (1.7%) of human stools yielded ESBL-producing E coli. Dominant genes were bla(CTX–M–55) and bla(CTX–M–27) in corvid, bla(CTX–M–15) and bla(CTX–M–27) in human isolates. ST162 was common among rooks. Two rook-derived E. coli belonged to ST131 C1-M27, which was also predominant (10/42) among human fecal and (15/42) human clinical isolates. Another potential link between rooks and humans was a single ST744 rook isolate grouped with one human fecal and three clinical isolates. Despite possible contact, genotypes shared between rooks and humans were rare. Thus, rooks are important as long-distance vectors and reservoirs of ESBL-producing E. coli rather than direct sources of infections to humans in our setting. |
format | Online Article Text |
id | pubmed-8792927 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-87929272022-01-28 Comparison of Extended-Spectrum Beta-Lactamase-Producing Escherichia coli Isolates From Rooks (Corvus frugilegus) and Contemporary Human-Derived Strains: A One Health Perspective Nagy, Bálint József Balázs, Bence Benmazouz, Isma Gyüre, Péter Kövér, László Kaszab, Eszter Bali, Krisztina Lovas-Kiss, Ádám Damjanova, Ivelina Majoros, László Tóth, Ákos Bányai, Krisztián Kardos, Gábor Front Microbiol Microbiology During winter, a large number of rooks gather and defecate at the park of a university clinic. We investigated the prevalence of extended-spectrum beta-lactamase (ESBL)–producing Escherichia coli in these birds and compared recovered isolates with contemporary human isolates. In 2016, fecal samples were collected from 112 trap-captured rooks and investigated for presence of ESBL producers using eosin methylene blue agar supplemented by 2 mg/L cefotaxime; 2,455 contemporary human fecal samples of patients of the clinics sent for routine culturing were tested similarly. In addition, 42 ESBL-producing E. coli isolates collected during the same period from inpatients were also studied. ESBL genes were sought for by PCR and were characterized by sequencing; E. coli ST131 clones were identified. Epidemiological relatedness was determined by pulsed-field gel electrophoresis and confirmed using whole genome sequencing in selected cases. Thirty-seven (33%) of sampled rooks and 42 (1.7%) of human stools yielded ESBL-producing E coli. Dominant genes were bla(CTX–M–55) and bla(CTX–M–27) in corvid, bla(CTX–M–15) and bla(CTX–M–27) in human isolates. ST162 was common among rooks. Two rook-derived E. coli belonged to ST131 C1-M27, which was also predominant (10/42) among human fecal and (15/42) human clinical isolates. Another potential link between rooks and humans was a single ST744 rook isolate grouped with one human fecal and three clinical isolates. Despite possible contact, genotypes shared between rooks and humans were rare. Thus, rooks are important as long-distance vectors and reservoirs of ESBL-producing E. coli rather than direct sources of infections to humans in our setting. Frontiers Media S.A. 2022-01-13 /pmc/articles/PMC8792927/ /pubmed/35095799 http://dx.doi.org/10.3389/fmicb.2021.785411 Text en Copyright © 2022 Nagy, Balázs, Benmazouz, Gyüre, Kövér, Kaszab, Bali, Lovas-Kiss, Damjanova, Majoros, Tóth, Bányai and Kardos. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Nagy, Bálint József Balázs, Bence Benmazouz, Isma Gyüre, Péter Kövér, László Kaszab, Eszter Bali, Krisztina Lovas-Kiss, Ádám Damjanova, Ivelina Majoros, László Tóth, Ákos Bányai, Krisztián Kardos, Gábor Comparison of Extended-Spectrum Beta-Lactamase-Producing Escherichia coli Isolates From Rooks (Corvus frugilegus) and Contemporary Human-Derived Strains: A One Health Perspective |
title | Comparison of Extended-Spectrum Beta-Lactamase-Producing Escherichia coli Isolates From Rooks (Corvus frugilegus) and Contemporary Human-Derived Strains: A One Health Perspective |
title_full | Comparison of Extended-Spectrum Beta-Lactamase-Producing Escherichia coli Isolates From Rooks (Corvus frugilegus) and Contemporary Human-Derived Strains: A One Health Perspective |
title_fullStr | Comparison of Extended-Spectrum Beta-Lactamase-Producing Escherichia coli Isolates From Rooks (Corvus frugilegus) and Contemporary Human-Derived Strains: A One Health Perspective |
title_full_unstemmed | Comparison of Extended-Spectrum Beta-Lactamase-Producing Escherichia coli Isolates From Rooks (Corvus frugilegus) and Contemporary Human-Derived Strains: A One Health Perspective |
title_short | Comparison of Extended-Spectrum Beta-Lactamase-Producing Escherichia coli Isolates From Rooks (Corvus frugilegus) and Contemporary Human-Derived Strains: A One Health Perspective |
title_sort | comparison of extended-spectrum beta-lactamase-producing escherichia coli isolates from rooks (corvus frugilegus) and contemporary human-derived strains: a one health perspective |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8792927/ https://www.ncbi.nlm.nih.gov/pubmed/35095799 http://dx.doi.org/10.3389/fmicb.2021.785411 |
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