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Guilingji Protects Against Spermatogenesis Dysfunction From Oxidative Stress via Regulation of MAPK and Apoptotic Signaling Pathways in Immp2l Mutant Mice

Male infertility is a major health issue with an estimated prevalence of 4.2% of male infertility worldwide. Oxidative stress (OS) is one of the main causes of male infertility, which is characterized by excessive reactive oxygen species (ROS) or lack of antioxidants. Meanwhile, it is reported that...

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Autores principales: Wang, Zhenqing, Xie, Yun, Chen, Haicheng, Yao, Jiahui, Lv, Linyan, Li, Yanqing, Deng, Chunhua, Zhang, Min, Sun, Xiangzhou, Liu, Guihua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8793631/
https://www.ncbi.nlm.nih.gov/pubmed/35095490
http://dx.doi.org/10.3389/fphar.2021.771161
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author Wang, Zhenqing
Xie, Yun
Chen, Haicheng
Yao, Jiahui
Lv, Linyan
Li, Yanqing
Deng, Chunhua
Zhang, Min
Sun, Xiangzhou
Liu, Guihua
author_facet Wang, Zhenqing
Xie, Yun
Chen, Haicheng
Yao, Jiahui
Lv, Linyan
Li, Yanqing
Deng, Chunhua
Zhang, Min
Sun, Xiangzhou
Liu, Guihua
author_sort Wang, Zhenqing
collection PubMed
description Male infertility is a major health issue with an estimated prevalence of 4.2% of male infertility worldwide. Oxidative stress (OS) is one of the main causes of male infertility, which is characterized by excessive reactive oxygen species (ROS) or lack of antioxidants. Meanwhile, it is reported that oxidative stress plays an important role in the spermatogenic impairment in Inner mitochondrial membrane peptidase 2-like (Immp2l) mutant mice. In this study, we focused on the potential mechanism of Guilingji in protecting the spermatogenic functions in Immp2l mutant mice. The results revealed that Immp2l mutant mice exhibit impaired spermatogenesis and histology shows seminiferous tubules with reduced spermatogenic cells. After administration of Guilingji [150 mg/kg per day intragastric gavage], however, alleviated spermatogenesis impairment and reversed testis histopathological damage and reduced apoptosis. What’s more, western blotting and the levels of redox classic markers revealed that Guilingji can markedly reduce reactive oxygen species. Moreover, Guilingji treatment led to inhibition of the phosphorylation of mitogen-activated protein kinase (MAPK), regulated apoptosis in the cells. In summary, Guilingji can improve spermatogenesis in Immp2l mutant mice by regulating oxidation-antioxidant balance and MAPK pathway. Our data suggests that Guilingji may be a promising and effective antioxidant candidate for the treatment of male infertility.
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spelling pubmed-87936312022-01-28 Guilingji Protects Against Spermatogenesis Dysfunction From Oxidative Stress via Regulation of MAPK and Apoptotic Signaling Pathways in Immp2l Mutant Mice Wang, Zhenqing Xie, Yun Chen, Haicheng Yao, Jiahui Lv, Linyan Li, Yanqing Deng, Chunhua Zhang, Min Sun, Xiangzhou Liu, Guihua Front Pharmacol Pharmacology Male infertility is a major health issue with an estimated prevalence of 4.2% of male infertility worldwide. Oxidative stress (OS) is one of the main causes of male infertility, which is characterized by excessive reactive oxygen species (ROS) or lack of antioxidants. Meanwhile, it is reported that oxidative stress plays an important role in the spermatogenic impairment in Inner mitochondrial membrane peptidase 2-like (Immp2l) mutant mice. In this study, we focused on the potential mechanism of Guilingji in protecting the spermatogenic functions in Immp2l mutant mice. The results revealed that Immp2l mutant mice exhibit impaired spermatogenesis and histology shows seminiferous tubules with reduced spermatogenic cells. After administration of Guilingji [150 mg/kg per day intragastric gavage], however, alleviated spermatogenesis impairment and reversed testis histopathological damage and reduced apoptosis. What’s more, western blotting and the levels of redox classic markers revealed that Guilingji can markedly reduce reactive oxygen species. Moreover, Guilingji treatment led to inhibition of the phosphorylation of mitogen-activated protein kinase (MAPK), regulated apoptosis in the cells. In summary, Guilingji can improve spermatogenesis in Immp2l mutant mice by regulating oxidation-antioxidant balance and MAPK pathway. Our data suggests that Guilingji may be a promising and effective antioxidant candidate for the treatment of male infertility. Frontiers Media S.A. 2022-01-13 /pmc/articles/PMC8793631/ /pubmed/35095490 http://dx.doi.org/10.3389/fphar.2021.771161 Text en Copyright © 2022 Wang, Xie, Chen, Yao, Lv, Li, Deng, Zhang, Sun and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Wang, Zhenqing
Xie, Yun
Chen, Haicheng
Yao, Jiahui
Lv, Linyan
Li, Yanqing
Deng, Chunhua
Zhang, Min
Sun, Xiangzhou
Liu, Guihua
Guilingji Protects Against Spermatogenesis Dysfunction From Oxidative Stress via Regulation of MAPK and Apoptotic Signaling Pathways in Immp2l Mutant Mice
title Guilingji Protects Against Spermatogenesis Dysfunction From Oxidative Stress via Regulation of MAPK and Apoptotic Signaling Pathways in Immp2l Mutant Mice
title_full Guilingji Protects Against Spermatogenesis Dysfunction From Oxidative Stress via Regulation of MAPK and Apoptotic Signaling Pathways in Immp2l Mutant Mice
title_fullStr Guilingji Protects Against Spermatogenesis Dysfunction From Oxidative Stress via Regulation of MAPK and Apoptotic Signaling Pathways in Immp2l Mutant Mice
title_full_unstemmed Guilingji Protects Against Spermatogenesis Dysfunction From Oxidative Stress via Regulation of MAPK and Apoptotic Signaling Pathways in Immp2l Mutant Mice
title_short Guilingji Protects Against Spermatogenesis Dysfunction From Oxidative Stress via Regulation of MAPK and Apoptotic Signaling Pathways in Immp2l Mutant Mice
title_sort guilingji protects against spermatogenesis dysfunction from oxidative stress via regulation of mapk and apoptotic signaling pathways in immp2l mutant mice
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8793631/
https://www.ncbi.nlm.nih.gov/pubmed/35095490
http://dx.doi.org/10.3389/fphar.2021.771161
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