Cargando…

Multifarious Indigenous Diazotrophic Rhizobacteria of Rice (Oryza sativa L.) Rhizosphere and Their Effect on Plant Growth Promotion

A diverse group of rhizobacteria persists in the rhizospheric soil, on the surface of roots, or in association with rice plants. These bacteria colonize plant root systems, enhance plant growth and crop yield. Indigenous rhizobacteria are known to promote soil health, grain production quality and se...

Descripción completa

Detalles Bibliográficos
Autores principales: Mir, Mohammad Imran, Hameeda, Bee, Quadriya, Humera, Kumar, B. Kiran, Ilyas, Noshin, Kee Zuan, Ali Tan, El Enshasy, Hesham Ali, Dailin, Daniel Joe, Kassem, Hazem S., Gafur, Abdul, Sayyed, R. Z.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8793879/
https://www.ncbi.nlm.nih.gov/pubmed/35096930
http://dx.doi.org/10.3389/fnut.2021.781764
_version_ 1784640704267419648
author Mir, Mohammad Imran
Hameeda, Bee
Quadriya, Humera
Kumar, B. Kiran
Ilyas, Noshin
Kee Zuan, Ali Tan
El Enshasy, Hesham Ali
Dailin, Daniel Joe
Kassem, Hazem S.
Gafur, Abdul
Sayyed, R. Z.
author_facet Mir, Mohammad Imran
Hameeda, Bee
Quadriya, Humera
Kumar, B. Kiran
Ilyas, Noshin
Kee Zuan, Ali Tan
El Enshasy, Hesham Ali
Dailin, Daniel Joe
Kassem, Hazem S.
Gafur, Abdul
Sayyed, R. Z.
author_sort Mir, Mohammad Imran
collection PubMed
description A diverse group of rhizobacteria persists in the rhizospheric soil, on the surface of roots, or in association with rice plants. These bacteria colonize plant root systems, enhance plant growth and crop yield. Indigenous rhizobacteria are known to promote soil health, grain production quality and serve as sustainable bioinoculant. The present study was aimed to isolate, identify and characterize indigenous plant growth promoting (PGP) diazotrophic bacteria associated with the rhizosphere of rice fields from different areas of Jammu and Kashmir, India. A total of 15 bacteria were isolated and evaluated for various PGP traits, antagonistic activity against phytopathogens, production of hydrolytic enzymes and biofilm formation under in-vitro conditions. The majority of the isolated bacteria were Gram-negative. Out of 15 bacterial isolates, nine isolates produced IAA (12.24 ± 2.86 to 250.3 ± 1.15 μg/ml), 6 isolates exhibited phosphate solubilization activity (36.69 ± 1.63 to 312.4 ± 1.15 μg/ml), 7 isolates exhibited rock phosphate solubilization while 5 isolates solubilized zinc (10–18 mm), 7 isolates showed siderophore production, 8 isolates exhibited HCN production, 6 isolates exhibited aminocyclopropane-1-carboxylate (ACC) deaminase activity, 13 isolates exhibited cellulase activity, nine isolates exhibited amylase and lipase activity and six isolates exhibited chitinase activity. In addition, 5 isolates showed amplification with the nifH gene and showed a significant amount of nitrogenase activity in a range of 0.127–4.39 μmol C(2)H(4)/mg protein/h. Five isolates viz., IHK-1, IHK-3, IHK-13, IHK-15 and IHK-25 exhibited most PGP attributes and successfully limited the mycelial growth of Rhizoctonia solani and Fusarium oxysporum in-vitro. All the five bacterial isolates were identified based on morphological, biochemical and 16S rDNA gene sequencing study, as Stenotrophomonas maltophilia, Enterobacter sp., Bacillus sp., Ochrobactrum haematophilum and Pseudomonas aeruginosa. Rice plants developed from seeds inoculated with these PGP strains individually had considerably higher germination percentage, seed vigor index and total dry biomass when compared to control. These findings strongly imply that the PGP diazotrophic bacteria identified in this work could be employed as plant growth stimulators in rice.
format Online
Article
Text
id pubmed-8793879
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-87938792022-01-28 Multifarious Indigenous Diazotrophic Rhizobacteria of Rice (Oryza sativa L.) Rhizosphere and Their Effect on Plant Growth Promotion Mir, Mohammad Imran Hameeda, Bee Quadriya, Humera Kumar, B. Kiran Ilyas, Noshin Kee Zuan, Ali Tan El Enshasy, Hesham Ali Dailin, Daniel Joe Kassem, Hazem S. Gafur, Abdul Sayyed, R. Z. Front Nutr Nutrition A diverse group of rhizobacteria persists in the rhizospheric soil, on the surface of roots, or in association with rice plants. These bacteria colonize plant root systems, enhance plant growth and crop yield. Indigenous rhizobacteria are known to promote soil health, grain production quality and serve as sustainable bioinoculant. The present study was aimed to isolate, identify and characterize indigenous plant growth promoting (PGP) diazotrophic bacteria associated with the rhizosphere of rice fields from different areas of Jammu and Kashmir, India. A total of 15 bacteria were isolated and evaluated for various PGP traits, antagonistic activity against phytopathogens, production of hydrolytic enzymes and biofilm formation under in-vitro conditions. The majority of the isolated bacteria were Gram-negative. Out of 15 bacterial isolates, nine isolates produced IAA (12.24 ± 2.86 to 250.3 ± 1.15 μg/ml), 6 isolates exhibited phosphate solubilization activity (36.69 ± 1.63 to 312.4 ± 1.15 μg/ml), 7 isolates exhibited rock phosphate solubilization while 5 isolates solubilized zinc (10–18 mm), 7 isolates showed siderophore production, 8 isolates exhibited HCN production, 6 isolates exhibited aminocyclopropane-1-carboxylate (ACC) deaminase activity, 13 isolates exhibited cellulase activity, nine isolates exhibited amylase and lipase activity and six isolates exhibited chitinase activity. In addition, 5 isolates showed amplification with the nifH gene and showed a significant amount of nitrogenase activity in a range of 0.127–4.39 μmol C(2)H(4)/mg protein/h. Five isolates viz., IHK-1, IHK-3, IHK-13, IHK-15 and IHK-25 exhibited most PGP attributes and successfully limited the mycelial growth of Rhizoctonia solani and Fusarium oxysporum in-vitro. All the five bacterial isolates were identified based on morphological, biochemical and 16S rDNA gene sequencing study, as Stenotrophomonas maltophilia, Enterobacter sp., Bacillus sp., Ochrobactrum haematophilum and Pseudomonas aeruginosa. Rice plants developed from seeds inoculated with these PGP strains individually had considerably higher germination percentage, seed vigor index and total dry biomass when compared to control. These findings strongly imply that the PGP diazotrophic bacteria identified in this work could be employed as plant growth stimulators in rice. Frontiers Media S.A. 2022-01-13 /pmc/articles/PMC8793879/ /pubmed/35096930 http://dx.doi.org/10.3389/fnut.2021.781764 Text en Copyright © 2022 Mir, Hameeda, Quadriya, Kumar, Ilyas, Kee Zuan, El Enshasy, Dailin, Kassem, Gafur and Sayyed. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Nutrition
Mir, Mohammad Imran
Hameeda, Bee
Quadriya, Humera
Kumar, B. Kiran
Ilyas, Noshin
Kee Zuan, Ali Tan
El Enshasy, Hesham Ali
Dailin, Daniel Joe
Kassem, Hazem S.
Gafur, Abdul
Sayyed, R. Z.
Multifarious Indigenous Diazotrophic Rhizobacteria of Rice (Oryza sativa L.) Rhizosphere and Their Effect on Plant Growth Promotion
title Multifarious Indigenous Diazotrophic Rhizobacteria of Rice (Oryza sativa L.) Rhizosphere and Their Effect on Plant Growth Promotion
title_full Multifarious Indigenous Diazotrophic Rhizobacteria of Rice (Oryza sativa L.) Rhizosphere and Their Effect on Plant Growth Promotion
title_fullStr Multifarious Indigenous Diazotrophic Rhizobacteria of Rice (Oryza sativa L.) Rhizosphere and Their Effect on Plant Growth Promotion
title_full_unstemmed Multifarious Indigenous Diazotrophic Rhizobacteria of Rice (Oryza sativa L.) Rhizosphere and Their Effect on Plant Growth Promotion
title_short Multifarious Indigenous Diazotrophic Rhizobacteria of Rice (Oryza sativa L.) Rhizosphere and Their Effect on Plant Growth Promotion
title_sort multifarious indigenous diazotrophic rhizobacteria of rice (oryza sativa l.) rhizosphere and their effect on plant growth promotion
topic Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8793879/
https://www.ncbi.nlm.nih.gov/pubmed/35096930
http://dx.doi.org/10.3389/fnut.2021.781764
work_keys_str_mv AT mirmohammadimran multifariousindigenousdiazotrophicrhizobacteriaofriceoryzasativalrhizosphereandtheireffectonplantgrowthpromotion
AT hameedabee multifariousindigenousdiazotrophicrhizobacteriaofriceoryzasativalrhizosphereandtheireffectonplantgrowthpromotion
AT quadriyahumera multifariousindigenousdiazotrophicrhizobacteriaofriceoryzasativalrhizosphereandtheireffectonplantgrowthpromotion
AT kumarbkiran multifariousindigenousdiazotrophicrhizobacteriaofriceoryzasativalrhizosphereandtheireffectonplantgrowthpromotion
AT ilyasnoshin multifariousindigenousdiazotrophicrhizobacteriaofriceoryzasativalrhizosphereandtheireffectonplantgrowthpromotion
AT keezuanalitan multifariousindigenousdiazotrophicrhizobacteriaofriceoryzasativalrhizosphereandtheireffectonplantgrowthpromotion
AT elenshasyheshamali multifariousindigenousdiazotrophicrhizobacteriaofriceoryzasativalrhizosphereandtheireffectonplantgrowthpromotion
AT dailindanieljoe multifariousindigenousdiazotrophicrhizobacteriaofriceoryzasativalrhizosphereandtheireffectonplantgrowthpromotion
AT kassemhazems multifariousindigenousdiazotrophicrhizobacteriaofriceoryzasativalrhizosphereandtheireffectonplantgrowthpromotion
AT gafurabdul multifariousindigenousdiazotrophicrhizobacteriaofriceoryzasativalrhizosphereandtheireffectonplantgrowthpromotion
AT sayyedrz multifariousindigenousdiazotrophicrhizobacteriaofriceoryzasativalrhizosphereandtheireffectonplantgrowthpromotion