Cargando…

Involvement of enhanced expression of classical complement C1q in atherosclerosis progression and plaque instability: C1q as an indicator of clinical outcome

Activation of the classical complement pathway plays a major role in regulating atherosclerosis progression, and it is believed to have both proatherogenic and atheroprotective effects. This study focused on C1q, the first protein in the classical pathway, and examined its potentialities of plaque p...

Descripción completa

Detalles Bibliográficos
Autores principales: Sasaki, Shoh, Nishihira, Kensaku, Yamashita, Atsushi, Fujii, Tomomi, Onoue, Kenji, Saito, Yoshihiko, Hatakeyama, Kinta, Shibata, Yoshisato, Asada, Yujiro, Ohbayashi, Chiho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8794146/
https://www.ncbi.nlm.nih.gov/pubmed/35085285
http://dx.doi.org/10.1371/journal.pone.0262413
_version_ 1784640765843996672
author Sasaki, Shoh
Nishihira, Kensaku
Yamashita, Atsushi
Fujii, Tomomi
Onoue, Kenji
Saito, Yoshihiko
Hatakeyama, Kinta
Shibata, Yoshisato
Asada, Yujiro
Ohbayashi, Chiho
author_facet Sasaki, Shoh
Nishihira, Kensaku
Yamashita, Atsushi
Fujii, Tomomi
Onoue, Kenji
Saito, Yoshihiko
Hatakeyama, Kinta
Shibata, Yoshisato
Asada, Yujiro
Ohbayashi, Chiho
author_sort Sasaki, Shoh
collection PubMed
description Activation of the classical complement pathway plays a major role in regulating atherosclerosis progression, and it is believed to have both proatherogenic and atheroprotective effects. This study focused on C1q, the first protein in the classical pathway, and examined its potentialities of plaque progression and instability and its relationship with clinical outcomes. To assess the localization and quantity of C1q expression in various stages of atherosclerosis, immunohistochemistry, western blotting, and real-time polymerase chain reaction (PCR) were performed using abdominal aortas from eight autopsy cases. C1q immunoreactivity in relation to plaque instability and clinical outcomes was also examined using directional coronary atherectomy (DCA) samples from 19 patients with acute coronary syndromes (ACS) and 18 patients with stable angina pectoris (SAP) and coronary aspirated specimens from 38 patients with acute myocardial infarction. C1q immunoreactivity was localized in the extracellular matrix, necrotic cores, macrophages and smooth muscle cells in atherosclerotic lesions. Western blotting and real-time PCR illustrated that C1q protein and mRNA expression was significantly higher in advanced lesions than in early lesions. Immunohistochemical analysis using DCA specimens revealed that C1q expression was significantly higher in ACS plaques than in SAP plaques. Finally, immunohistochemical analysis using thrombus aspiration specimens demonstrated that histopathological C1q in aspirated coronary materials could be an indicator of poor medical condition. Our results indicated that C1q is significantly involved in atherosclerosis progression and plaque instability, and it could be considered as one of the indicators of cardiovascular outcomes.
format Online
Article
Text
id pubmed-8794146
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-87941462022-01-28 Involvement of enhanced expression of classical complement C1q in atherosclerosis progression and plaque instability: C1q as an indicator of clinical outcome Sasaki, Shoh Nishihira, Kensaku Yamashita, Atsushi Fujii, Tomomi Onoue, Kenji Saito, Yoshihiko Hatakeyama, Kinta Shibata, Yoshisato Asada, Yujiro Ohbayashi, Chiho PLoS One Research Article Activation of the classical complement pathway plays a major role in regulating atherosclerosis progression, and it is believed to have both proatherogenic and atheroprotective effects. This study focused on C1q, the first protein in the classical pathway, and examined its potentialities of plaque progression and instability and its relationship with clinical outcomes. To assess the localization and quantity of C1q expression in various stages of atherosclerosis, immunohistochemistry, western blotting, and real-time polymerase chain reaction (PCR) were performed using abdominal aortas from eight autopsy cases. C1q immunoreactivity in relation to plaque instability and clinical outcomes was also examined using directional coronary atherectomy (DCA) samples from 19 patients with acute coronary syndromes (ACS) and 18 patients with stable angina pectoris (SAP) and coronary aspirated specimens from 38 patients with acute myocardial infarction. C1q immunoreactivity was localized in the extracellular matrix, necrotic cores, macrophages and smooth muscle cells in atherosclerotic lesions. Western blotting and real-time PCR illustrated that C1q protein and mRNA expression was significantly higher in advanced lesions than in early lesions. Immunohistochemical analysis using DCA specimens revealed that C1q expression was significantly higher in ACS plaques than in SAP plaques. Finally, immunohistochemical analysis using thrombus aspiration specimens demonstrated that histopathological C1q in aspirated coronary materials could be an indicator of poor medical condition. Our results indicated that C1q is significantly involved in atherosclerosis progression and plaque instability, and it could be considered as one of the indicators of cardiovascular outcomes. Public Library of Science 2022-01-27 /pmc/articles/PMC8794146/ /pubmed/35085285 http://dx.doi.org/10.1371/journal.pone.0262413 Text en © 2022 Sasaki et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Sasaki, Shoh
Nishihira, Kensaku
Yamashita, Atsushi
Fujii, Tomomi
Onoue, Kenji
Saito, Yoshihiko
Hatakeyama, Kinta
Shibata, Yoshisato
Asada, Yujiro
Ohbayashi, Chiho
Involvement of enhanced expression of classical complement C1q in atherosclerosis progression and plaque instability: C1q as an indicator of clinical outcome
title Involvement of enhanced expression of classical complement C1q in atherosclerosis progression and plaque instability: C1q as an indicator of clinical outcome
title_full Involvement of enhanced expression of classical complement C1q in atherosclerosis progression and plaque instability: C1q as an indicator of clinical outcome
title_fullStr Involvement of enhanced expression of classical complement C1q in atherosclerosis progression and plaque instability: C1q as an indicator of clinical outcome
title_full_unstemmed Involvement of enhanced expression of classical complement C1q in atherosclerosis progression and plaque instability: C1q as an indicator of clinical outcome
title_short Involvement of enhanced expression of classical complement C1q in atherosclerosis progression and plaque instability: C1q as an indicator of clinical outcome
title_sort involvement of enhanced expression of classical complement c1q in atherosclerosis progression and plaque instability: c1q as an indicator of clinical outcome
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8794146/
https://www.ncbi.nlm.nih.gov/pubmed/35085285
http://dx.doi.org/10.1371/journal.pone.0262413
work_keys_str_mv AT sasakishoh involvementofenhancedexpressionofclassicalcomplementc1qinatherosclerosisprogressionandplaqueinstabilityc1qasanindicatorofclinicaloutcome
AT nishihirakensaku involvementofenhancedexpressionofclassicalcomplementc1qinatherosclerosisprogressionandplaqueinstabilityc1qasanindicatorofclinicaloutcome
AT yamashitaatsushi involvementofenhancedexpressionofclassicalcomplementc1qinatherosclerosisprogressionandplaqueinstabilityc1qasanindicatorofclinicaloutcome
AT fujiitomomi involvementofenhancedexpressionofclassicalcomplementc1qinatherosclerosisprogressionandplaqueinstabilityc1qasanindicatorofclinicaloutcome
AT onouekenji involvementofenhancedexpressionofclassicalcomplementc1qinatherosclerosisprogressionandplaqueinstabilityc1qasanindicatorofclinicaloutcome
AT saitoyoshihiko involvementofenhancedexpressionofclassicalcomplementc1qinatherosclerosisprogressionandplaqueinstabilityc1qasanindicatorofclinicaloutcome
AT hatakeyamakinta involvementofenhancedexpressionofclassicalcomplementc1qinatherosclerosisprogressionandplaqueinstabilityc1qasanindicatorofclinicaloutcome
AT shibatayoshisato involvementofenhancedexpressionofclassicalcomplementc1qinatherosclerosisprogressionandplaqueinstabilityc1qasanindicatorofclinicaloutcome
AT asadayujiro involvementofenhancedexpressionofclassicalcomplementc1qinatherosclerosisprogressionandplaqueinstabilityc1qasanindicatorofclinicaloutcome
AT ohbayashichiho involvementofenhancedexpressionofclassicalcomplementc1qinatherosclerosisprogressionandplaqueinstabilityc1qasanindicatorofclinicaloutcome