Cargando…
Metabolic Understanding of the Genetic Dysregulation in the Tumor Microenvironment of Kidney Renal Clear Cell Carcinoma
The metabolic dysregulation is a hallmark of cancers including KIRC, specifically caused by alterations in metabolic genes. Currently, a lack of consensus exists between metabolic signatures in the tumor microenvironment. Here, in this study, we observed the significant correlations of differentiall...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8794690/ https://www.ncbi.nlm.nih.gov/pubmed/35096203 http://dx.doi.org/10.1155/2022/6085072 |
_version_ | 1784640874900094976 |
---|---|
author | Xie, Junwei Cui, Lingang Pan, Shaokang Liu, Dongwei Liu, Fengxun Liu, Zhangsuo |
author_facet | Xie, Junwei Cui, Lingang Pan, Shaokang Liu, Dongwei Liu, Fengxun Liu, Zhangsuo |
author_sort | Xie, Junwei |
collection | PubMed |
description | The metabolic dysregulation is a hallmark of cancers including KIRC, specifically caused by alterations in metabolic genes. Currently, a lack of consensus exists between metabolic signatures in the tumor microenvironment. Here, in this study, we observed the significant correlations of differentially expressed metabolic genes (DEmGs) between KIRC and the related normal samples. Briefly, we collected sets of metabolic genes through RNA-seq data of KIRC and normal tissues from TCGA, followed by the identification of KIRC-related DEmGs. Next, patients were classified into three clusters, and using WGCNA, we identified metabolic genes involved in the survival among different clusters. Furthermore, we investigated survival and clinical parameters along with immune infiltration in the clusters. At the same time, we constructed and validated a prediction model based on these DEmGs. These analyses revealed that the patients having high expression of DEmGs showed poor survival, while infiltration of less-immune cells was associated with the metastasis of KIRC. In the end, we identified NUDT1 as a hub gene as it showed significantly high expression in KIRC samples as well as associated with the survival and prognosis of the patients. Further analysis revealed the oncogenic role of NUDT1 in 786-O and ACHN cells. Thus, we conclude that NUDT1 could be a potential diagnostic and prognostic marker for KIRC. |
format | Online Article Text |
id | pubmed-8794690 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-87946902022-01-28 Metabolic Understanding of the Genetic Dysregulation in the Tumor Microenvironment of Kidney Renal Clear Cell Carcinoma Xie, Junwei Cui, Lingang Pan, Shaokang Liu, Dongwei Liu, Fengxun Liu, Zhangsuo Dis Markers Research Article The metabolic dysregulation is a hallmark of cancers including KIRC, specifically caused by alterations in metabolic genes. Currently, a lack of consensus exists between metabolic signatures in the tumor microenvironment. Here, in this study, we observed the significant correlations of differentially expressed metabolic genes (DEmGs) between KIRC and the related normal samples. Briefly, we collected sets of metabolic genes through RNA-seq data of KIRC and normal tissues from TCGA, followed by the identification of KIRC-related DEmGs. Next, patients were classified into three clusters, and using WGCNA, we identified metabolic genes involved in the survival among different clusters. Furthermore, we investigated survival and clinical parameters along with immune infiltration in the clusters. At the same time, we constructed and validated a prediction model based on these DEmGs. These analyses revealed that the patients having high expression of DEmGs showed poor survival, while infiltration of less-immune cells was associated with the metastasis of KIRC. In the end, we identified NUDT1 as a hub gene as it showed significantly high expression in KIRC samples as well as associated with the survival and prognosis of the patients. Further analysis revealed the oncogenic role of NUDT1 in 786-O and ACHN cells. Thus, we conclude that NUDT1 could be a potential diagnostic and prognostic marker for KIRC. Hindawi 2022-01-20 /pmc/articles/PMC8794690/ /pubmed/35096203 http://dx.doi.org/10.1155/2022/6085072 Text en Copyright © 2022 Junwei Xie et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Xie, Junwei Cui, Lingang Pan, Shaokang Liu, Dongwei Liu, Fengxun Liu, Zhangsuo Metabolic Understanding of the Genetic Dysregulation in the Tumor Microenvironment of Kidney Renal Clear Cell Carcinoma |
title | Metabolic Understanding of the Genetic Dysregulation in the Tumor Microenvironment of Kidney Renal Clear Cell Carcinoma |
title_full | Metabolic Understanding of the Genetic Dysregulation in the Tumor Microenvironment of Kidney Renal Clear Cell Carcinoma |
title_fullStr | Metabolic Understanding of the Genetic Dysregulation in the Tumor Microenvironment of Kidney Renal Clear Cell Carcinoma |
title_full_unstemmed | Metabolic Understanding of the Genetic Dysregulation in the Tumor Microenvironment of Kidney Renal Clear Cell Carcinoma |
title_short | Metabolic Understanding of the Genetic Dysregulation in the Tumor Microenvironment of Kidney Renal Clear Cell Carcinoma |
title_sort | metabolic understanding of the genetic dysregulation in the tumor microenvironment of kidney renal clear cell carcinoma |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8794690/ https://www.ncbi.nlm.nih.gov/pubmed/35096203 http://dx.doi.org/10.1155/2022/6085072 |
work_keys_str_mv | AT xiejunwei metabolicunderstandingofthegeneticdysregulationinthetumormicroenvironmentofkidneyrenalclearcellcarcinoma AT cuilingang metabolicunderstandingofthegeneticdysregulationinthetumormicroenvironmentofkidneyrenalclearcellcarcinoma AT panshaokang metabolicunderstandingofthegeneticdysregulationinthetumormicroenvironmentofkidneyrenalclearcellcarcinoma AT liudongwei metabolicunderstandingofthegeneticdysregulationinthetumormicroenvironmentofkidneyrenalclearcellcarcinoma AT liufengxun metabolicunderstandingofthegeneticdysregulationinthetumormicroenvironmentofkidneyrenalclearcellcarcinoma AT liuzhangsuo metabolicunderstandingofthegeneticdysregulationinthetumormicroenvironmentofkidneyrenalclearcellcarcinoma |