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A Metagenomics Investigation of Intergenerational Effects of Non-nutritive Sweeteners on Gut Microbiome

To identify possible mechanisms by which maternal consumption of non-nutritive sweeteners increases obesity risk in offspring, we reconstructed the major alterations in the cecal microbiome of 3-week-old offspring of obese dams consuming high fat/sucrose (HFS) diet with or without aspartame (5–7 mg/...

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Autores principales: Wang, Weilan, Nettleton, Jodi E., Gänzle, Michael G., Reimer, Raylene A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8794796/
https://www.ncbi.nlm.nih.gov/pubmed/35096940
http://dx.doi.org/10.3389/fnut.2021.795848
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author Wang, Weilan
Nettleton, Jodi E.
Gänzle, Michael G.
Reimer, Raylene A.
author_facet Wang, Weilan
Nettleton, Jodi E.
Gänzle, Michael G.
Reimer, Raylene A.
author_sort Wang, Weilan
collection PubMed
description To identify possible mechanisms by which maternal consumption of non-nutritive sweeteners increases obesity risk in offspring, we reconstructed the major alterations in the cecal microbiome of 3-week-old offspring of obese dams consuming high fat/sucrose (HFS) diet with or without aspartame (5–7 mg/kg/day) or stevia (2–3 mg/kg/day) by shotgun metagenomic sequencing (n = 36). High throughput 16S rRNA gene sequencing (n = 105) was performed for dams, 3- and 18-week-old offspring. Maternal consumption of sweeteners altered cecal microbial composition and metabolism of propionate/lactate in their offspring. Offspring daily body weight gain, liver weight and body fat were positively correlated to the relative abundance of key microbes and enzymes involved in succinate/propionate production while negatively correlated to that of lactose degradation and lactate production. The altered propionate/lactate production in the cecum of weanlings from aspartame and stevia consuming dams implicates an altered ratio of dietary carbohydrate digestion, mainly lactose, in the small intestine vs. microbial fermentation in the large intestine. The reconstructed microbiome alterations could explain increased offspring body weight and body fat. This study demonstrates that intense sweet tastants have a lasting and intergenerational effect on gut microbiota, microbial metabolites and host health.
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spelling pubmed-87947962022-01-29 A Metagenomics Investigation of Intergenerational Effects of Non-nutritive Sweeteners on Gut Microbiome Wang, Weilan Nettleton, Jodi E. Gänzle, Michael G. Reimer, Raylene A. Front Nutr Nutrition To identify possible mechanisms by which maternal consumption of non-nutritive sweeteners increases obesity risk in offspring, we reconstructed the major alterations in the cecal microbiome of 3-week-old offspring of obese dams consuming high fat/sucrose (HFS) diet with or without aspartame (5–7 mg/kg/day) or stevia (2–3 mg/kg/day) by shotgun metagenomic sequencing (n = 36). High throughput 16S rRNA gene sequencing (n = 105) was performed for dams, 3- and 18-week-old offspring. Maternal consumption of sweeteners altered cecal microbial composition and metabolism of propionate/lactate in their offspring. Offspring daily body weight gain, liver weight and body fat were positively correlated to the relative abundance of key microbes and enzymes involved in succinate/propionate production while negatively correlated to that of lactose degradation and lactate production. The altered propionate/lactate production in the cecum of weanlings from aspartame and stevia consuming dams implicates an altered ratio of dietary carbohydrate digestion, mainly lactose, in the small intestine vs. microbial fermentation in the large intestine. The reconstructed microbiome alterations could explain increased offspring body weight and body fat. This study demonstrates that intense sweet tastants have a lasting and intergenerational effect on gut microbiota, microbial metabolites and host health. Frontiers Media S.A. 2022-01-14 /pmc/articles/PMC8794796/ /pubmed/35096940 http://dx.doi.org/10.3389/fnut.2021.795848 Text en Copyright © 2022 Wang, Nettleton, Gänzle and Reimer. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Nutrition
Wang, Weilan
Nettleton, Jodi E.
Gänzle, Michael G.
Reimer, Raylene A.
A Metagenomics Investigation of Intergenerational Effects of Non-nutritive Sweeteners on Gut Microbiome
title A Metagenomics Investigation of Intergenerational Effects of Non-nutritive Sweeteners on Gut Microbiome
title_full A Metagenomics Investigation of Intergenerational Effects of Non-nutritive Sweeteners on Gut Microbiome
title_fullStr A Metagenomics Investigation of Intergenerational Effects of Non-nutritive Sweeteners on Gut Microbiome
title_full_unstemmed A Metagenomics Investigation of Intergenerational Effects of Non-nutritive Sweeteners on Gut Microbiome
title_short A Metagenomics Investigation of Intergenerational Effects of Non-nutritive Sweeteners on Gut Microbiome
title_sort metagenomics investigation of intergenerational effects of non-nutritive sweeteners on gut microbiome
topic Nutrition
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8794796/
https://www.ncbi.nlm.nih.gov/pubmed/35096940
http://dx.doi.org/10.3389/fnut.2021.795848
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