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CRISPR/Cas12a-Assisted Visual Logic-Gate Detection of Pathogenic Microorganisms Based on Water-Soluble DNA-Binding AIEgens

Here, we developed a rapid, visual and double-checked Logic Gate detection platform for detection of pathogenic microorganisms by aggregation-induced emission luminogens (AIEgens) in combination with Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR associated (Cas). DNA ligh...

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Autores principales: Jiao, Zhe, Yang, Jialing, Long, Xiaojuan, Lu, Yingfang, Guo, Zongning, Peng, Yonglin, Huang, Xuelin, Yin, Yi, Song, Chao, Zhang, Pengfei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8795674/
https://www.ncbi.nlm.nih.gov/pubmed/35096768
http://dx.doi.org/10.3389/fchem.2021.801972
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author Jiao, Zhe
Yang, Jialing
Long, Xiaojuan
Lu, Yingfang
Guo, Zongning
Peng, Yonglin
Huang, Xuelin
Yin, Yi
Song, Chao
Zhang, Pengfei
author_facet Jiao, Zhe
Yang, Jialing
Long, Xiaojuan
Lu, Yingfang
Guo, Zongning
Peng, Yonglin
Huang, Xuelin
Yin, Yi
Song, Chao
Zhang, Pengfei
author_sort Jiao, Zhe
collection PubMed
description Here, we developed a rapid, visual and double-checked Logic Gate detection platform for detection of pathogenic microorganisms by aggregation-induced emission luminogens (AIEgens) in combination with Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR associated (Cas). DNA light-up AIEgens (1,1,2,2-tetrakis[4-(2-bromo-ethoxy) phenyl]ethene, TTAPE) was non-emissive but the emission was turned on in the presence of large amount of DNA produced by recombinase polymerase amplification (RPA). When CRISPR/Cas12a was added, all long-stranded DNA were cut leading to the emission quenched. Thus, a method that can directly observe the emission changes with the naked eye has been successfully constructed. The detection is speedy within only 20 min, and has strong specificity to the target. The result can be judged by Logic Gate. Only when the output signal is (1,0), does it represent the presence of pathogenic microorganisms in the test object. Finally, the method was applied to the detect pathogenic microorganisms in environmental water samples, which proved that this method has high selectivity, specificity and applicability for the detection of pathogenic microorganisms in environmental water samples.
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spelling pubmed-87956742022-01-29 CRISPR/Cas12a-Assisted Visual Logic-Gate Detection of Pathogenic Microorganisms Based on Water-Soluble DNA-Binding AIEgens Jiao, Zhe Yang, Jialing Long, Xiaojuan Lu, Yingfang Guo, Zongning Peng, Yonglin Huang, Xuelin Yin, Yi Song, Chao Zhang, Pengfei Front Chem Chemistry Here, we developed a rapid, visual and double-checked Logic Gate detection platform for detection of pathogenic microorganisms by aggregation-induced emission luminogens (AIEgens) in combination with Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR associated (Cas). DNA light-up AIEgens (1,1,2,2-tetrakis[4-(2-bromo-ethoxy) phenyl]ethene, TTAPE) was non-emissive but the emission was turned on in the presence of large amount of DNA produced by recombinase polymerase amplification (RPA). When CRISPR/Cas12a was added, all long-stranded DNA were cut leading to the emission quenched. Thus, a method that can directly observe the emission changes with the naked eye has been successfully constructed. The detection is speedy within only 20 min, and has strong specificity to the target. The result can be judged by Logic Gate. Only when the output signal is (1,0), does it represent the presence of pathogenic microorganisms in the test object. Finally, the method was applied to the detect pathogenic microorganisms in environmental water samples, which proved that this method has high selectivity, specificity and applicability for the detection of pathogenic microorganisms in environmental water samples. Frontiers Media S.A. 2022-01-14 /pmc/articles/PMC8795674/ /pubmed/35096768 http://dx.doi.org/10.3389/fchem.2021.801972 Text en Copyright © 2022 Jiao, Yang, Long, Lu, Guo, Peng, Huang, Yin, Song and Zhang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Chemistry
Jiao, Zhe
Yang, Jialing
Long, Xiaojuan
Lu, Yingfang
Guo, Zongning
Peng, Yonglin
Huang, Xuelin
Yin, Yi
Song, Chao
Zhang, Pengfei
CRISPR/Cas12a-Assisted Visual Logic-Gate Detection of Pathogenic Microorganisms Based on Water-Soluble DNA-Binding AIEgens
title CRISPR/Cas12a-Assisted Visual Logic-Gate Detection of Pathogenic Microorganisms Based on Water-Soluble DNA-Binding AIEgens
title_full CRISPR/Cas12a-Assisted Visual Logic-Gate Detection of Pathogenic Microorganisms Based on Water-Soluble DNA-Binding AIEgens
title_fullStr CRISPR/Cas12a-Assisted Visual Logic-Gate Detection of Pathogenic Microorganisms Based on Water-Soluble DNA-Binding AIEgens
title_full_unstemmed CRISPR/Cas12a-Assisted Visual Logic-Gate Detection of Pathogenic Microorganisms Based on Water-Soluble DNA-Binding AIEgens
title_short CRISPR/Cas12a-Assisted Visual Logic-Gate Detection of Pathogenic Microorganisms Based on Water-Soluble DNA-Binding AIEgens
title_sort crispr/cas12a-assisted visual logic-gate detection of pathogenic microorganisms based on water-soluble dna-binding aiegens
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8795674/
https://www.ncbi.nlm.nih.gov/pubmed/35096768
http://dx.doi.org/10.3389/fchem.2021.801972
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