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Bioengineered pseudovirus nanoparticles displaying the HA1 antigens of influenza viruses for enhanced immunogenicity

Even with implementation of current influenza vaccines, influenza still claims up to 500,000 lives worldwide annually, indicating a need for a better vaccine strategy. We have developed a technology to generate unique S(60)-HA1 pseudovirus nanoparticles (PVNPs) that display the receptor-binding HA1...

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Autores principales: Xia, Ming, Hoq, Md Rejaul, Huang, Pengwei, Jiang, Wen, Jiang, Xi, Tan, Ming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tsinghua University Press 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8795936/
https://www.ncbi.nlm.nih.gov/pubmed/35106126
http://dx.doi.org/10.1007/s12274-021-4011-x
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author Xia, Ming
Hoq, Md Rejaul
Huang, Pengwei
Jiang, Wen
Jiang, Xi
Tan, Ming
author_facet Xia, Ming
Hoq, Md Rejaul
Huang, Pengwei
Jiang, Wen
Jiang, Xi
Tan, Ming
author_sort Xia, Ming
collection PubMed
description Even with implementation of current influenza vaccines, influenza still claims up to 500,000 lives worldwide annually, indicating a need for a better vaccine strategy. We have developed a technology to generate unique S(60)-HA1 pseudovirus nanoparticles (PVNPs) that display the receptor-binding HA1 domains of influenza viruses. Each self-assembled S(60)-HA1 PVNP consists of a T = 1 icosahedral S(60) nanoparticle that resembles the inner shell of norovirus capsid and 60 surface-displayed HA1 antigens that are excellent vaccine targets. Soluble S(60)-HA1 PVNPs presenting HA1 antigens of H7N9 influenza virus subtypes have been produced efficiently in large amount. Their three-dimensional (3D) structures have been solved by cryogenic electron microscopy. The PVNP-displayed HA1 antigens react with HA-specific antibody, and retain authentic sialic acid binding specificity and hemagglutinate human erythrocytes. The PVNPs are highly immunogenic, eliciting high titers of HA1-specific antibodies in mice and the mouse sera strongly inhibited hemagglutinations of homologous and heterologous influenza virus HA proteins. Therefore, the S(60)-HA1 PVNPs may provide useful reagents to study influenza viruses and offer a potential new vaccine tactic to fight the deadly influenza disease. [Image: see text]
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spelling pubmed-87959362022-01-28 Bioengineered pseudovirus nanoparticles displaying the HA1 antigens of influenza viruses for enhanced immunogenicity Xia, Ming Hoq, Md Rejaul Huang, Pengwei Jiang, Wen Jiang, Xi Tan, Ming Nano Res Research Article Even with implementation of current influenza vaccines, influenza still claims up to 500,000 lives worldwide annually, indicating a need for a better vaccine strategy. We have developed a technology to generate unique S(60)-HA1 pseudovirus nanoparticles (PVNPs) that display the receptor-binding HA1 domains of influenza viruses. Each self-assembled S(60)-HA1 PVNP consists of a T = 1 icosahedral S(60) nanoparticle that resembles the inner shell of norovirus capsid and 60 surface-displayed HA1 antigens that are excellent vaccine targets. Soluble S(60)-HA1 PVNPs presenting HA1 antigens of H7N9 influenza virus subtypes have been produced efficiently in large amount. Their three-dimensional (3D) structures have been solved by cryogenic electron microscopy. The PVNP-displayed HA1 antigens react with HA-specific antibody, and retain authentic sialic acid binding specificity and hemagglutinate human erythrocytes. The PVNPs are highly immunogenic, eliciting high titers of HA1-specific antibodies in mice and the mouse sera strongly inhibited hemagglutinations of homologous and heterologous influenza virus HA proteins. Therefore, the S(60)-HA1 PVNPs may provide useful reagents to study influenza viruses and offer a potential new vaccine tactic to fight the deadly influenza disease. [Image: see text] Tsinghua University Press 2022-01-28 2022 /pmc/articles/PMC8795936/ /pubmed/35106126 http://dx.doi.org/10.1007/s12274-021-4011-x Text en © Tsinghua University Press and Springer-Verlag GmbH Germany, part of Springer Nature 2022 This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Research Article
Xia, Ming
Hoq, Md Rejaul
Huang, Pengwei
Jiang, Wen
Jiang, Xi
Tan, Ming
Bioengineered pseudovirus nanoparticles displaying the HA1 antigens of influenza viruses for enhanced immunogenicity
title Bioengineered pseudovirus nanoparticles displaying the HA1 antigens of influenza viruses for enhanced immunogenicity
title_full Bioengineered pseudovirus nanoparticles displaying the HA1 antigens of influenza viruses for enhanced immunogenicity
title_fullStr Bioengineered pseudovirus nanoparticles displaying the HA1 antigens of influenza viruses for enhanced immunogenicity
title_full_unstemmed Bioengineered pseudovirus nanoparticles displaying the HA1 antigens of influenza viruses for enhanced immunogenicity
title_short Bioengineered pseudovirus nanoparticles displaying the HA1 antigens of influenza viruses for enhanced immunogenicity
title_sort bioengineered pseudovirus nanoparticles displaying the ha1 antigens of influenza viruses for enhanced immunogenicity
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8795936/
https://www.ncbi.nlm.nih.gov/pubmed/35106126
http://dx.doi.org/10.1007/s12274-021-4011-x
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