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Enriched CD45RA(−)CD62L(+) central memory T and decreased CD3(+)CD56(+) natural killer T lymphocyte subsets in the rectum of ulcerative colitis patients
OBJECTIVES: To investigate the distinctive features of lymphocytes promoting inflammation in ulcerative colitis. METHODS: We performed flow cytometric analysis of peripheral blood mononuclear cells (PBMCs) and colorectal mucosa lymphocytes in ulcerative colitis patients (n = 13) and control patients...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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SAGE Publications
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8796091/ https://www.ncbi.nlm.nih.gov/pubmed/35021918 http://dx.doi.org/10.1177/20587384211051982 |
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author | Iwamuro, Masaya Takahashi, Takahide Watanabe, Natsuki Tanaka, Takehiro Inokuchi, Toshihiro Hiraoka, Sakiko Otsuka, Fumio Okada, Hiroyuki |
author_facet | Iwamuro, Masaya Takahashi, Takahide Watanabe, Natsuki Tanaka, Takehiro Inokuchi, Toshihiro Hiraoka, Sakiko Otsuka, Fumio Okada, Hiroyuki |
author_sort | Iwamuro, Masaya |
collection | PubMed |
description | OBJECTIVES: To investigate the distinctive features of lymphocytes promoting inflammation in ulcerative colitis. METHODS: We performed flow cytometric analysis of peripheral blood mononuclear cells (PBMCs) and colorectal mucosa lymphocytes in ulcerative colitis patients (n = 13) and control patients (n = 5). RESULTS: CD62L(+)/CD3(+)CD4(+) (35.7 ± 14.0% vs. 19.9 ± 6.4%) and CD62L(+)/CD3(+)CD4(−) cells (17.1 ± 17.4% vs. 2.4 ± 3.9%) were higher in the rectum of ulcerative colitis patients than in control patients. Subpopulation analysis revealed that CD45RA(−)CD62L(+)/CD3(+)CD4(+), that is, central memory T cell fraction in CD4(+) T cells, was significantly increased in the rectum of ulcerative colitis, compared to that in control patients (23.3 ± 10.5% vs. 8.2 ± 4.0%). Comparison of rectum and colon samples in ulcerative colitis patients indicated that CD56(+)/CD3(+) was decreased in the rectum compared to that in the colon (11.3 ± 12.5% vs. 21.3 ± 16.5%). The ratio of CD56(+)/CD3(+) was also decreased in the rectum of active ulcerative colitis patients compared to that in ulcerative colitis patients at the endoscopic remission stages (2.8 ± 1.7% vs. 18.5 ± 13.3%). CONCLUSION: We demonstrated that CD62L(+) T lymphocytes, particularly the CD45RA(−)CD62L(+) T cell subset that represents central memory T cells, were increased in the rectum of patients with ulcerative colitis. In addition, the CD56(+)/CD3(+) subset (natural killer T cells) was decreased in the rectum compared to that of less inflamed colonic mucosa. These results suggest that the enrichment of central memory T lymphocytes and the reduction of natural killer T cells in the gut mucosa are involved in the pathogenesis of ulcerative colitis. |
format | Online Article Text |
id | pubmed-8796091 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | SAGE Publications |
record_format | MEDLINE/PubMed |
spelling | pubmed-87960912022-01-29 Enriched CD45RA(−)CD62L(+) central memory T and decreased CD3(+)CD56(+) natural killer T lymphocyte subsets in the rectum of ulcerative colitis patients Iwamuro, Masaya Takahashi, Takahide Watanabe, Natsuki Tanaka, Takehiro Inokuchi, Toshihiro Hiraoka, Sakiko Otsuka, Fumio Okada, Hiroyuki Int J Immunopathol Pharmacol Original Research OBJECTIVES: To investigate the distinctive features of lymphocytes promoting inflammation in ulcerative colitis. METHODS: We performed flow cytometric analysis of peripheral blood mononuclear cells (PBMCs) and colorectal mucosa lymphocytes in ulcerative colitis patients (n = 13) and control patients (n = 5). RESULTS: CD62L(+)/CD3(+)CD4(+) (35.7 ± 14.0% vs. 19.9 ± 6.4%) and CD62L(+)/CD3(+)CD4(−) cells (17.1 ± 17.4% vs. 2.4 ± 3.9%) were higher in the rectum of ulcerative colitis patients than in control patients. Subpopulation analysis revealed that CD45RA(−)CD62L(+)/CD3(+)CD4(+), that is, central memory T cell fraction in CD4(+) T cells, was significantly increased in the rectum of ulcerative colitis, compared to that in control patients (23.3 ± 10.5% vs. 8.2 ± 4.0%). Comparison of rectum and colon samples in ulcerative colitis patients indicated that CD56(+)/CD3(+) was decreased in the rectum compared to that in the colon (11.3 ± 12.5% vs. 21.3 ± 16.5%). The ratio of CD56(+)/CD3(+) was also decreased in the rectum of active ulcerative colitis patients compared to that in ulcerative colitis patients at the endoscopic remission stages (2.8 ± 1.7% vs. 18.5 ± 13.3%). CONCLUSION: We demonstrated that CD62L(+) T lymphocytes, particularly the CD45RA(−)CD62L(+) T cell subset that represents central memory T cells, were increased in the rectum of patients with ulcerative colitis. In addition, the CD56(+)/CD3(+) subset (natural killer T cells) was decreased in the rectum compared to that of less inflamed colonic mucosa. These results suggest that the enrichment of central memory T lymphocytes and the reduction of natural killer T cells in the gut mucosa are involved in the pathogenesis of ulcerative colitis. SAGE Publications 2022-01-12 /pmc/articles/PMC8796091/ /pubmed/35021918 http://dx.doi.org/10.1177/20587384211051982 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by-nc/4.0/This article is distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 License (https://creativecommons.org/licenses/by-nc/4.0/) which permits non-commercial use, reproduction and distribution of the work without further permission provided the original work is attributed as specified on the SAGE and Open Access pages (https://us.sagepub.com/en-us/nam/open-access-at-sage). |
spellingShingle | Original Research Iwamuro, Masaya Takahashi, Takahide Watanabe, Natsuki Tanaka, Takehiro Inokuchi, Toshihiro Hiraoka, Sakiko Otsuka, Fumio Okada, Hiroyuki Enriched CD45RA(−)CD62L(+) central memory T and decreased CD3(+)CD56(+) natural killer T lymphocyte subsets in the rectum of ulcerative colitis patients |
title | Enriched CD45RA(−)CD62L(+) central memory T and decreased CD3(+)CD56(+) natural killer T lymphocyte subsets in the rectum of ulcerative colitis patients |
title_full | Enriched CD45RA(−)CD62L(+) central memory T and decreased CD3(+)CD56(+) natural killer T lymphocyte subsets in the rectum of ulcerative colitis patients |
title_fullStr | Enriched CD45RA(−)CD62L(+) central memory T and decreased CD3(+)CD56(+) natural killer T lymphocyte subsets in the rectum of ulcerative colitis patients |
title_full_unstemmed | Enriched CD45RA(−)CD62L(+) central memory T and decreased CD3(+)CD56(+) natural killer T lymphocyte subsets in the rectum of ulcerative colitis patients |
title_short | Enriched CD45RA(−)CD62L(+) central memory T and decreased CD3(+)CD56(+) natural killer T lymphocyte subsets in the rectum of ulcerative colitis patients |
title_sort | enriched cd45ra(−)cd62l(+) central memory t and decreased cd3(+)cd56(+) natural killer t lymphocyte subsets in the rectum of ulcerative colitis patients |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8796091/ https://www.ncbi.nlm.nih.gov/pubmed/35021918 http://dx.doi.org/10.1177/20587384211051982 |
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