Cargando…

Fe-Curcumin Nanozyme-Mediated Reactive Oxygen Species Scavenging and Anti-Inflammation for Acute Lung Injury

[Image: see text] Pneumonia, such as acute lung injury (ALI), has been a type of lethal disease that is generally caused by uncontrolled inflammatory response and excessive generation of reactive oxygen species (ROS). Herein, we report Fe-curcumin-based nanoparticles (Fe-Cur NPs) with nanozyme funct...

Descripción completa

Detalles Bibliográficos
Autores principales: Yuan, Renyikun, Li, Yuqing, Han, Shan, Chen, Xinxin, Chen, Jingqi, He, Jia, Gao, Hongwei, Yang, Yang, Yang, Shilin, Yang, Yu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2021
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8796308/
https://www.ncbi.nlm.nih.gov/pubmed/35106369
http://dx.doi.org/10.1021/acscentsci.1c00866
_version_ 1784641276866461696
author Yuan, Renyikun
Li, Yuqing
Han, Shan
Chen, Xinxin
Chen, Jingqi
He, Jia
Gao, Hongwei
Yang, Yang
Yang, Shilin
Yang, Yu
author_facet Yuan, Renyikun
Li, Yuqing
Han, Shan
Chen, Xinxin
Chen, Jingqi
He, Jia
Gao, Hongwei
Yang, Yang
Yang, Shilin
Yang, Yu
author_sort Yuan, Renyikun
collection PubMed
description [Image: see text] Pneumonia, such as acute lung injury (ALI), has been a type of lethal disease that is generally caused by uncontrolled inflammatory response and excessive generation of reactive oxygen species (ROS). Herein, we report Fe-curcumin-based nanoparticles (Fe-Cur NPs) with nanozyme functionalities in guiding the intracellular ROS scavenging and meanwhile exhibiting anti-inflammation efficacy for curing ALI. The nanoparticles are noncytotoxic when directing these biological activities. Mechanism studies for the anti-inflammation aspects of Fe-Cur NPs were systematically carried out, in which the infected cells and tissues were alleviated through downregulating levels of several important inflammatory cytokines (such as TNF-α, IL-1β, and IL-6), decreasing the intracellular Ca(2+) release, inhibiting NLRP3 inflammasomes, and suppressing NF-κB signaling pathways. In addition, we performed both the intratracheal and intravenous injection of Fe-Cur NPs in mice experiencing ALI and, importantly, found that the accumulation of such nanozymes was enhanced in lung tissue (better than free curcumin drugs), demonstrating its promising therapeutic efficiency in two different administration methods. We showed that the inflammation reduction of Fe-Cur NPs was effective in animal experiments and that ROS scavenging was also effectively achieved in lung tissue. Finally, we revealed that Fe-Cur NPs can decrease the level of macrophage cells (CD11b(lo)F4/80(hi)) and CD3(+)CD45(+) T cells in mice, which could help suppress the inflammation cytokine storm caused by ALI. Overall, this work has developed the strategy of using Fe-Cur NPs as nanozymes to scavenge intracellular ROS and as an anti-inflammation nanodrugs to synergistically cure ALI, which may serve as a promising therapeutic agent in the clinical treatment of this deadly disease. Fe-Cur NP nanozymes were designed to attenuate ALI by clearing intracellular ROS and alleviating inflammation synergistically. Relevant cytokines, inflammasomes, and signaling pathways were studied.
format Online
Article
Text
id pubmed-8796308
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher American Chemical Society
record_format MEDLINE/PubMed
spelling pubmed-87963082022-01-31 Fe-Curcumin Nanozyme-Mediated Reactive Oxygen Species Scavenging and Anti-Inflammation for Acute Lung Injury Yuan, Renyikun Li, Yuqing Han, Shan Chen, Xinxin Chen, Jingqi He, Jia Gao, Hongwei Yang, Yang Yang, Shilin Yang, Yu ACS Cent Sci [Image: see text] Pneumonia, such as acute lung injury (ALI), has been a type of lethal disease that is generally caused by uncontrolled inflammatory response and excessive generation of reactive oxygen species (ROS). Herein, we report Fe-curcumin-based nanoparticles (Fe-Cur NPs) with nanozyme functionalities in guiding the intracellular ROS scavenging and meanwhile exhibiting anti-inflammation efficacy for curing ALI. The nanoparticles are noncytotoxic when directing these biological activities. Mechanism studies for the anti-inflammation aspects of Fe-Cur NPs were systematically carried out, in which the infected cells and tissues were alleviated through downregulating levels of several important inflammatory cytokines (such as TNF-α, IL-1β, and IL-6), decreasing the intracellular Ca(2+) release, inhibiting NLRP3 inflammasomes, and suppressing NF-κB signaling pathways. In addition, we performed both the intratracheal and intravenous injection of Fe-Cur NPs in mice experiencing ALI and, importantly, found that the accumulation of such nanozymes was enhanced in lung tissue (better than free curcumin drugs), demonstrating its promising therapeutic efficiency in two different administration methods. We showed that the inflammation reduction of Fe-Cur NPs was effective in animal experiments and that ROS scavenging was also effectively achieved in lung tissue. Finally, we revealed that Fe-Cur NPs can decrease the level of macrophage cells (CD11b(lo)F4/80(hi)) and CD3(+)CD45(+) T cells in mice, which could help suppress the inflammation cytokine storm caused by ALI. Overall, this work has developed the strategy of using Fe-Cur NPs as nanozymes to scavenge intracellular ROS and as an anti-inflammation nanodrugs to synergistically cure ALI, which may serve as a promising therapeutic agent in the clinical treatment of this deadly disease. Fe-Cur NP nanozymes were designed to attenuate ALI by clearing intracellular ROS and alleviating inflammation synergistically. Relevant cytokines, inflammasomes, and signaling pathways were studied. American Chemical Society 2021-12-02 2022-01-26 /pmc/articles/PMC8796308/ /pubmed/35106369 http://dx.doi.org/10.1021/acscentsci.1c00866 Text en © 2021 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Yuan, Renyikun
Li, Yuqing
Han, Shan
Chen, Xinxin
Chen, Jingqi
He, Jia
Gao, Hongwei
Yang, Yang
Yang, Shilin
Yang, Yu
Fe-Curcumin Nanozyme-Mediated Reactive Oxygen Species Scavenging and Anti-Inflammation for Acute Lung Injury
title Fe-Curcumin Nanozyme-Mediated Reactive Oxygen Species Scavenging and Anti-Inflammation for Acute Lung Injury
title_full Fe-Curcumin Nanozyme-Mediated Reactive Oxygen Species Scavenging and Anti-Inflammation for Acute Lung Injury
title_fullStr Fe-Curcumin Nanozyme-Mediated Reactive Oxygen Species Scavenging and Anti-Inflammation for Acute Lung Injury
title_full_unstemmed Fe-Curcumin Nanozyme-Mediated Reactive Oxygen Species Scavenging and Anti-Inflammation for Acute Lung Injury
title_short Fe-Curcumin Nanozyme-Mediated Reactive Oxygen Species Scavenging and Anti-Inflammation for Acute Lung Injury
title_sort fe-curcumin nanozyme-mediated reactive oxygen species scavenging and anti-inflammation for acute lung injury
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8796308/
https://www.ncbi.nlm.nih.gov/pubmed/35106369
http://dx.doi.org/10.1021/acscentsci.1c00866
work_keys_str_mv AT yuanrenyikun fecurcuminnanozymemediatedreactiveoxygenspeciesscavengingandantiinflammationforacutelunginjury
AT liyuqing fecurcuminnanozymemediatedreactiveoxygenspeciesscavengingandantiinflammationforacutelunginjury
AT hanshan fecurcuminnanozymemediatedreactiveoxygenspeciesscavengingandantiinflammationforacutelunginjury
AT chenxinxin fecurcuminnanozymemediatedreactiveoxygenspeciesscavengingandantiinflammationforacutelunginjury
AT chenjingqi fecurcuminnanozymemediatedreactiveoxygenspeciesscavengingandantiinflammationforacutelunginjury
AT hejia fecurcuminnanozymemediatedreactiveoxygenspeciesscavengingandantiinflammationforacutelunginjury
AT gaohongwei fecurcuminnanozymemediatedreactiveoxygenspeciesscavengingandantiinflammationforacutelunginjury
AT yangyang fecurcuminnanozymemediatedreactiveoxygenspeciesscavengingandantiinflammationforacutelunginjury
AT yangshilin fecurcuminnanozymemediatedreactiveoxygenspeciesscavengingandantiinflammationforacutelunginjury
AT yangyu fecurcuminnanozymemediatedreactiveoxygenspeciesscavengingandantiinflammationforacutelunginjury