Cargando…

Metabarcoding reveals massive species diversity of Diptera in a subtropical ecosystem

Diptera is often considered to be the richest insect group due to its great species diversity and broad ecological versatility. However, data on dipteran diversity from subtropical ecosystems have hitherto been scarce, due to the lack of studies conducted at an appropriate large scale. We investigat...

Descripción completa

Detalles Bibliográficos
Autores principales: Huang, Junhao, Miao, Xiaoqian, Wang, Qingyun, Menzel, Frank, Tang, Pu, Yang, Ding, Wu, Hong, Vogler, Alfried P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8796913/
https://www.ncbi.nlm.nih.gov/pubmed/35127039
http://dx.doi.org/10.1002/ece3.8535
_version_ 1784641422529396736
author Huang, Junhao
Miao, Xiaoqian
Wang, Qingyun
Menzel, Frank
Tang, Pu
Yang, Ding
Wu, Hong
Vogler, Alfried P.
author_facet Huang, Junhao
Miao, Xiaoqian
Wang, Qingyun
Menzel, Frank
Tang, Pu
Yang, Ding
Wu, Hong
Vogler, Alfried P.
author_sort Huang, Junhao
collection PubMed
description Diptera is often considered to be the richest insect group due to its great species diversity and broad ecological versatility. However, data on dipteran diversity from subtropical ecosystems have hitherto been scarce, due to the lack of studies conducted at an appropriate large scale. We investigated the diversity and composition of Diptera communities on Tianmu Mountain, Zhejiang, China, using DNA metabarcoding technology, and evaluated their dynamic responses to the effects of slope aspect, season, and altitudinal zone. A total of 5,092 operational taxonomic units (OTUs) were discovered and tentatively assigned to 72 dipteran families, including 2 family records new for China and 30 family records new for the locality. Cecidomyiidae, Sciaridae, and Phoridae were the predominant families, representing 53.6% of total OTUs, while 52 families include >95% unidentified and presumed undescribed species. We found that the community structure of Diptera was significantly affected by aspect, seasonality (month) and elevation, with richer diversity harbored in north‐facing than south‐facing slopes, and seasonality a more profound driver of community structure and diversity than elevation. Overall, massive species diversity of Diptera communities was discovered in this subtropical ecosystem of east China. The huge diversity of potentially undescribed species only revealed by metabarcoding now requires more detailed taxonomic study, as a step toward an evolutionary integration that accumulates information on species’ geographic ranges, ecological traits, functional roles, and species interactions, and thus places the local communities in the context of the growing knowledge base of global biodiversity and its response to environmental change.
format Online
Article
Text
id pubmed-8796913
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-87969132022-02-04 Metabarcoding reveals massive species diversity of Diptera in a subtropical ecosystem Huang, Junhao Miao, Xiaoqian Wang, Qingyun Menzel, Frank Tang, Pu Yang, Ding Wu, Hong Vogler, Alfried P. Ecol Evol Research Articles Diptera is often considered to be the richest insect group due to its great species diversity and broad ecological versatility. However, data on dipteran diversity from subtropical ecosystems have hitherto been scarce, due to the lack of studies conducted at an appropriate large scale. We investigated the diversity and composition of Diptera communities on Tianmu Mountain, Zhejiang, China, using DNA metabarcoding technology, and evaluated their dynamic responses to the effects of slope aspect, season, and altitudinal zone. A total of 5,092 operational taxonomic units (OTUs) were discovered and tentatively assigned to 72 dipteran families, including 2 family records new for China and 30 family records new for the locality. Cecidomyiidae, Sciaridae, and Phoridae were the predominant families, representing 53.6% of total OTUs, while 52 families include >95% unidentified and presumed undescribed species. We found that the community structure of Diptera was significantly affected by aspect, seasonality (month) and elevation, with richer diversity harbored in north‐facing than south‐facing slopes, and seasonality a more profound driver of community structure and diversity than elevation. Overall, massive species diversity of Diptera communities was discovered in this subtropical ecosystem of east China. The huge diversity of potentially undescribed species only revealed by metabarcoding now requires more detailed taxonomic study, as a step toward an evolutionary integration that accumulates information on species’ geographic ranges, ecological traits, functional roles, and species interactions, and thus places the local communities in the context of the growing knowledge base of global biodiversity and its response to environmental change. John Wiley and Sons Inc. 2022-01-23 /pmc/articles/PMC8796913/ /pubmed/35127039 http://dx.doi.org/10.1002/ece3.8535 Text en © 2022 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Huang, Junhao
Miao, Xiaoqian
Wang, Qingyun
Menzel, Frank
Tang, Pu
Yang, Ding
Wu, Hong
Vogler, Alfried P.
Metabarcoding reveals massive species diversity of Diptera in a subtropical ecosystem
title Metabarcoding reveals massive species diversity of Diptera in a subtropical ecosystem
title_full Metabarcoding reveals massive species diversity of Diptera in a subtropical ecosystem
title_fullStr Metabarcoding reveals massive species diversity of Diptera in a subtropical ecosystem
title_full_unstemmed Metabarcoding reveals massive species diversity of Diptera in a subtropical ecosystem
title_short Metabarcoding reveals massive species diversity of Diptera in a subtropical ecosystem
title_sort metabarcoding reveals massive species diversity of diptera in a subtropical ecosystem
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8796913/
https://www.ncbi.nlm.nih.gov/pubmed/35127039
http://dx.doi.org/10.1002/ece3.8535
work_keys_str_mv AT huangjunhao metabarcodingrevealsmassivespeciesdiversityofdipterainasubtropicalecosystem
AT miaoxiaoqian metabarcodingrevealsmassivespeciesdiversityofdipterainasubtropicalecosystem
AT wangqingyun metabarcodingrevealsmassivespeciesdiversityofdipterainasubtropicalecosystem
AT menzelfrank metabarcodingrevealsmassivespeciesdiversityofdipterainasubtropicalecosystem
AT tangpu metabarcodingrevealsmassivespeciesdiversityofdipterainasubtropicalecosystem
AT yangding metabarcodingrevealsmassivespeciesdiversityofdipterainasubtropicalecosystem
AT wuhong metabarcodingrevealsmassivespeciesdiversityofdipterainasubtropicalecosystem
AT vogleralfriedp metabarcodingrevealsmassivespeciesdiversityofdipterainasubtropicalecosystem