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Novel hair snare and genetic methods for non‐invasive bobcat detection
Over the past 20 years, the use of non‐invasive hair snare surveys in wildlife research and management has become more prevalent. While these tools have been used to answer important research questions, these techniques often fail to gather information on elusive carnivores, such as bobcats (Lynx ru...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8796956/ https://www.ncbi.nlm.nih.gov/pubmed/35127004 http://dx.doi.org/10.1002/ece3.8435 |
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author | Rounsville, Thomas F. Rogers, Richard E. Welsh, Amy B. Ryan, Christopher W. Anderson, James T. |
author_facet | Rounsville, Thomas F. Rogers, Richard E. Welsh, Amy B. Ryan, Christopher W. Anderson, James T. |
author_sort | Rounsville, Thomas F. |
collection | PubMed |
description | Over the past 20 years, the use of non‐invasive hair snare surveys in wildlife research and management has become more prevalent. While these tools have been used to answer important research questions, these techniques often fail to gather information on elusive carnivores, such as bobcats (Lynx rufus). Due to the limited success of previous bobcat studies using hair snares which required active rubbing, this technique has largely fallen out of use, in favor of camera trapping. The goal of our study was to construct a novel, passive bobcat hair snare that could be deployed regardless of terrain or vegetation features, which would be effective for use in capture–recapture population estimation at a large spatial scale. This new hair snare was deployed in 1500 10‐km(2) cells across West Virginia (USA) between two sampling seasons (2015–2016). Collected hair samples were analyzed with newly developed mitochondrial DNA primers specifically for felids and qPCR to determine species of origin, with enough sensitivity to identify samples as small as two bobcat hairs. Over the two years of the study, a total of 378 bobcat detections were recorded from 42,000 trap nights of sampling, for an overall rate of 0.9 detections/100 trap nights—nearly 2–6 times greater than any previous bobcat hair snare study. While the overall number of recaptured animals was low (n = 9), continued development of this platform should increase its usefulness in capture–recapture studies. |
format | Online Article Text |
id | pubmed-8796956 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-87969562022-02-04 Novel hair snare and genetic methods for non‐invasive bobcat detection Rounsville, Thomas F. Rogers, Richard E. Welsh, Amy B. Ryan, Christopher W. Anderson, James T. Ecol Evol Research Articles Over the past 20 years, the use of non‐invasive hair snare surveys in wildlife research and management has become more prevalent. While these tools have been used to answer important research questions, these techniques often fail to gather information on elusive carnivores, such as bobcats (Lynx rufus). Due to the limited success of previous bobcat studies using hair snares which required active rubbing, this technique has largely fallen out of use, in favor of camera trapping. The goal of our study was to construct a novel, passive bobcat hair snare that could be deployed regardless of terrain or vegetation features, which would be effective for use in capture–recapture population estimation at a large spatial scale. This new hair snare was deployed in 1500 10‐km(2) cells across West Virginia (USA) between two sampling seasons (2015–2016). Collected hair samples were analyzed with newly developed mitochondrial DNA primers specifically for felids and qPCR to determine species of origin, with enough sensitivity to identify samples as small as two bobcat hairs. Over the two years of the study, a total of 378 bobcat detections were recorded from 42,000 trap nights of sampling, for an overall rate of 0.9 detections/100 trap nights—nearly 2–6 times greater than any previous bobcat hair snare study. While the overall number of recaptured animals was low (n = 9), continued development of this platform should increase its usefulness in capture–recapture studies. John Wiley and Sons Inc. 2022-01-24 /pmc/articles/PMC8796956/ /pubmed/35127004 http://dx.doi.org/10.1002/ece3.8435 Text en © 2022 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Articles Rounsville, Thomas F. Rogers, Richard E. Welsh, Amy B. Ryan, Christopher W. Anderson, James T. Novel hair snare and genetic methods for non‐invasive bobcat detection |
title | Novel hair snare and genetic methods for non‐invasive bobcat detection |
title_full | Novel hair snare and genetic methods for non‐invasive bobcat detection |
title_fullStr | Novel hair snare and genetic methods for non‐invasive bobcat detection |
title_full_unstemmed | Novel hair snare and genetic methods for non‐invasive bobcat detection |
title_short | Novel hair snare and genetic methods for non‐invasive bobcat detection |
title_sort | novel hair snare and genetic methods for non‐invasive bobcat detection |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8796956/ https://www.ncbi.nlm.nih.gov/pubmed/35127004 http://dx.doi.org/10.1002/ece3.8435 |
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