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A Two-tiered functional screen identifies herpesviral transcriptional modifiers and their essential domains

While traditional methods for studying large DNA viruses allow the creation of individual mutants, CRISPR/Cas9 can be used to rapidly create thousands of mutant dsDNA viruses in parallel, enabling the pooled screening of entire viral genomes. Here, we applied this approach to Kaposi’s sarcoma-associ...

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Detalles Bibliográficos
Autores principales:	Morgens, David W., Nandakumar, Divya, Didychuk, Allison L., Yang, Kevin J., Glaunsinger, Britt A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2022
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8797222/ https://www.ncbi.nlm.nih.gov/pubmed/35041709 http://dx.doi.org/10.1371/journal.ppat.1010236

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