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MicroRNA-124 and microRNA-378 inhibit the proliferation and invasion of colorectal cancer by upregulating KiSS1

BACKGROUND: The KiSS1 gene is considered a tumor suppressor in various cancers. MicroRNAs are involved in many important life processes, and their regulation of gene expression may be as important as that of transcription factors. Here, we explore the roles of miR-124-3p and miR-378-3p in colorectal...

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Autores principales: Zheng, Yan, Liu, Yisu, Lin, Yilin, Lin, Suyong, Gao, Ji, Chen, Zhihua, Chen, Shaoqin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AME Publishing Company 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8797710/
https://www.ncbi.nlm.nih.gov/pubmed/35117640
http://dx.doi.org/10.21037/tcr.2020.02.30
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author Zheng, Yan
Liu, Yisu
Lin, Yilin
Lin, Suyong
Gao, Ji
Chen, Zhihua
Chen, Shaoqin
author_facet Zheng, Yan
Liu, Yisu
Lin, Yilin
Lin, Suyong
Gao, Ji
Chen, Zhihua
Chen, Shaoqin
author_sort Zheng, Yan
collection PubMed
description BACKGROUND: The KiSS1 gene is considered a tumor suppressor in various cancers. MicroRNAs are involved in many important life processes, and their regulation of gene expression may be as important as that of transcription factors. Here, we explore the roles of miR-124-3p and miR-378-3p in colorectal cancer and their relationships with the KiSS1 gene. METHODS: The effects of miR-124-3p and miR-378-3p on KiSS1 protein expression were observed by transfecting colorectal cancer cells (SW-480) with miR-124-3p and miR-378-3p mimics and inhibitors. Moreover, cell proliferation, migration and invasion were evaluated by ethynyl-20-deoxyuridine and Transwell experiments. RESULTS: The KiSS1 mRNA and protein expression levels were significantly increased in mimic-transfected cells compared with those in untransfected cells, and the proliferation, migration and invasion abilities of the former were decreased; in addition, opposing results were obtained in the inhibitor and mimic groups. CONCLUSIONS: In conclusion, our studies indicate that miR-124-3p and miR-378-3p upregulate the expression of KiSS1 and are associated with colorectal cancer metastasis and progression. miR-124-3p, miR-378-3p and KiSS1 may play important roles in colorectal cancer.
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spelling pubmed-87977102022-02-02 MicroRNA-124 and microRNA-378 inhibit the proliferation and invasion of colorectal cancer by upregulating KiSS1 Zheng, Yan Liu, Yisu Lin, Yilin Lin, Suyong Gao, Ji Chen, Zhihua Chen, Shaoqin Transl Cancer Res Original Article BACKGROUND: The KiSS1 gene is considered a tumor suppressor in various cancers. MicroRNAs are involved in many important life processes, and their regulation of gene expression may be as important as that of transcription factors. Here, we explore the roles of miR-124-3p and miR-378-3p in colorectal cancer and their relationships with the KiSS1 gene. METHODS: The effects of miR-124-3p and miR-378-3p on KiSS1 protein expression were observed by transfecting colorectal cancer cells (SW-480) with miR-124-3p and miR-378-3p mimics and inhibitors. Moreover, cell proliferation, migration and invasion were evaluated by ethynyl-20-deoxyuridine and Transwell experiments. RESULTS: The KiSS1 mRNA and protein expression levels were significantly increased in mimic-transfected cells compared with those in untransfected cells, and the proliferation, migration and invasion abilities of the former were decreased; in addition, opposing results were obtained in the inhibitor and mimic groups. CONCLUSIONS: In conclusion, our studies indicate that miR-124-3p and miR-378-3p upregulate the expression of KiSS1 and are associated with colorectal cancer metastasis and progression. miR-124-3p, miR-378-3p and KiSS1 may play important roles in colorectal cancer. AME Publishing Company 2020-04 /pmc/articles/PMC8797710/ /pubmed/35117640 http://dx.doi.org/10.21037/tcr.2020.02.30 Text en 2020 Translational Cancer Research. All rights reserved. https://creativecommons.org/licenses/by-nc-nd/4.0/Open Access Statement: This is an Open Access article distributed in accordance with the Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License (CC BY-NC-ND 4.0), which permits the non-commercial replication and distribution of the article with the strict proviso that no changes or edits are made and the original work is properly cited (including links to both the formal publication through the relevant DOI and the license). See: https://creativecommons.org/licenses/by-nc-nd/4.0/.
spellingShingle Original Article
Zheng, Yan
Liu, Yisu
Lin, Yilin
Lin, Suyong
Gao, Ji
Chen, Zhihua
Chen, Shaoqin
MicroRNA-124 and microRNA-378 inhibit the proliferation and invasion of colorectal cancer by upregulating KiSS1
title MicroRNA-124 and microRNA-378 inhibit the proliferation and invasion of colorectal cancer by upregulating KiSS1
title_full MicroRNA-124 and microRNA-378 inhibit the proliferation and invasion of colorectal cancer by upregulating KiSS1
title_fullStr MicroRNA-124 and microRNA-378 inhibit the proliferation and invasion of colorectal cancer by upregulating KiSS1
title_full_unstemmed MicroRNA-124 and microRNA-378 inhibit the proliferation and invasion of colorectal cancer by upregulating KiSS1
title_short MicroRNA-124 and microRNA-378 inhibit the proliferation and invasion of colorectal cancer by upregulating KiSS1
title_sort microrna-124 and microrna-378 inhibit the proliferation and invasion of colorectal cancer by upregulating kiss1
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8797710/
https://www.ncbi.nlm.nih.gov/pubmed/35117640
http://dx.doi.org/10.21037/tcr.2020.02.30
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